全文获取类型
收费全文 | 1209篇 |
免费 | 107篇 |
出版年
2023年 | 6篇 |
2022年 | 14篇 |
2021年 | 32篇 |
2020年 | 11篇 |
2019年 | 25篇 |
2018年 | 23篇 |
2017年 | 19篇 |
2016年 | 51篇 |
2015年 | 99篇 |
2014年 | 85篇 |
2013年 | 79篇 |
2012年 | 112篇 |
2011年 | 93篇 |
2010年 | 42篇 |
2009年 | 57篇 |
2008年 | 67篇 |
2007年 | 53篇 |
2006年 | 66篇 |
2005年 | 62篇 |
2004年 | 50篇 |
2003年 | 50篇 |
2002年 | 41篇 |
2001年 | 13篇 |
2000年 | 2篇 |
1999年 | 14篇 |
1998年 | 8篇 |
1997年 | 11篇 |
1996年 | 12篇 |
1995年 | 6篇 |
1994年 | 8篇 |
1993年 | 10篇 |
1992年 | 14篇 |
1991年 | 4篇 |
1990年 | 8篇 |
1988年 | 3篇 |
1987年 | 8篇 |
1985年 | 5篇 |
1984年 | 4篇 |
1983年 | 2篇 |
1982年 | 4篇 |
1981年 | 4篇 |
1980年 | 6篇 |
1977年 | 4篇 |
1974年 | 2篇 |
1972年 | 4篇 |
1970年 | 2篇 |
1959年 | 3篇 |
1955年 | 1篇 |
1954年 | 1篇 |
1949年 | 1篇 |
排序方式: 共有1316条查询结果,搜索用时 249 毫秒
141.
Wojcieszek JM Simmons LW 《Evolution; international journal of organic evolution》2012,66(4):1138-1153
It is generally accepted that postcopulatory sexual selection drives rapid divergence of genital morphology among isolated populations. The mode of selection operating upon genitalia can be explored by comparing patterns of population divergence in genetic and genitalic traits. We collected Antichiropus variabilis millipedes from eight localities across the species range. Levels of among-population genetic divergence, at microsatellite loci, and the mitochondrial COI gene were very high. Following geometric morphometric analyses, genital morphology was also found to be highly divergent among the populations surveyed, whereas head morphology had not diverged as markedly. However, pairwise comparisons of F(ST) and P(ST) showed that among-population divergence in both genital and head shape was significantly lower than that experienced by neutral genetic markers. Our results suggest that the genitalia of A. variabilis are currently experiencing a period of stabilizing selection, the mode of selection expected for genitalia that function in species recognition via a "lock-and-key" mechanism. Our results demonstrate that although genital morphology can clearly diverge among genetically isolated populations, divergence is not necessarily as rapid as commonly argued, and continuous directional sexual selection may not always underpin the evolutionary divergence of male genitalia. 相似文献
142.
Miloud T Fiegler N Suffner J Hämmerling GJ Garbi N 《Journal of immunology (Baltimore, Md. : 1950)》2012,188(3):1125-1135
Bone marrow-derived dendritic cell (DC) precursors seed peripheral organs, where they encounter diverse cellular environments during their final differentiation into DCs. Flt3 ligand (Flt3-L) is critical for instructing DC generation throughout different organs. However, it remains unknown which cells produce Flt3-L and, importantly, which cellular source drives DC development in such a variety of organs. Using a novel BAC transgenic Flt3-L reporter mouse strain coexpressing enhanced GFP and luciferase, we show ubiquitous Flt3-L expression in organs and cell types. These results were further confirmed at the protein level. Although Flt3-L was produced by immune and nonimmune cells, the source required for development of the DC compartment clearly differed among organs. In lymphoid organs such as the spleen and bone marrow, Flt3-L production by hemopoietic cells was critical for generation of normal DC numbers. This was unexpected for the spleen because both immune and nonimmune cells equally contributed to the Flt3-L content in that organ. Thus, localized production rather than the total tissue content of Flt3-L in spleen dictated normal splenic DC development. No differences were observed in the number of DC precursors, suggesting that the immune source of Flt3-L promoted pre-cDC differentiation in spleen. In contrast, DC generation in the lung, kidney, and pancreas was mostly driven by nonhematopoietic cells producing Flt3-L, with little contribution by immune cells. These findings demonstrate a high degree of flexibility in Flt3-L-dependent DC generation to adapt this process to organ-specific cellular environments encountered by DC precursors during their final differentiation. 相似文献
143.
144.
Joël Meunier Janine W. Y. Wong Yamenah Gómez Sabine Kuttler Lilian Röllin Dimitri Stucki Mathias Kölliker 《Evolutionary ecology》2012,26(3):669-682
Whether to reproduce once or multiple times (semelparity vs. iteroparity) is a major life-history decision that organisms
have to take. Mode of parity is usually considered a species characteristic. However, recent models suggested that population
properties or condition-dependent fitness payoffs could help to maintain both life-history tactics within populations. In
arthropods, semelparity was also hypothesised to be a critical pre-adaptation for the evolution of maternal care, semelparous
females being predicted to provide more care due to the absence of costs on future reproduction. The aim of this study was
to characterize potential fitness payoffs and levels of maternal care in semel- and itero-parous females of the European earwig
Forficula auricularia. Based on 15 traits measured in 494 females and their nymphs, our results revealed that iteroparous females laid their first
clutch earlier, had more eggs in their first clutch, gained more weight during the 2 weeks following hatching of the first
clutch, but produced eggs that developed more slowly than semelparous females. Among iteroparous females, the sizes of first
and second clutches were significantly and positively correlated, indicating no investment trade-off between reproductive
events. Iteroparous females also provided more food than semelparous ones, a result contrasting with predictions that iteroparity
is incompatible with the evolution of maternal care. Finally, a controlled breeding experiment reported full mating compatibility
among offspring from females of the two modes of parity, confirming that both types of females belong to one single species.
Overall, these results indicate that alternative modes of parity represent coexisting life-history tactics that are likely
to be condition-dependent and associated with offspring development and specific levels of maternal care in earwigs. 相似文献
145.
Procópio L Alvarez VM Jurelevicius DA Hansen L Sørensen SJ Cardoso JS Pádula M Leitão AC Seldin L van Elsas JD 《Antonie van Leeuwenhoek》2012,101(2):289-302
The draft genome of Dietzia cinnamea strain P4 was determined using pyrosequencing. In total, 428 supercontigs were obtained and analyzed. We here describe and
interpret the main features of the draft genome. The genome contained a total of 3,555,295 bp, arranged in a single replicon
with an average G+C percentage of 70.9%. It revealed the presence of complete pathways for basically all central metabolic
routes. Also present were complete sets of genes for the glyoxalate and reductive carboxylate cycles. Autotrophic growth was
suggested to occur by the presence of genes for aerobic CO oxidation, formate/formaldehyde oxidation, the reverse tricarboxylic
acid cycle and the 3-hydropropionate cycle for CO2 fixation. Secondary metabolism was evidenced by the presence of genes for the biosynthesis of terpene compounds, frenolicin,
nanaomycin and avilamycin A antibiotics. Furthermore, a probable role in azinomycin B synthesis, an important product with
antitumor activity, was indicated. The complete alk operon for the degradation of n-alkanes was found to be present, as were clusters of genes for biphenyl ring dihydroxylation. This study brings new insights
in the genetics and physiology of D. cinnamea P4, which is useful in biotechnology and bioremediation. 相似文献
146.
Khandros E Mollan TL Yu X Wang X Yao Y D'Souza J Gell DA Olson JS Weiss MJ 《The Journal of biological chemistry》2012,287(14):11325-11337
α-Hemoglobin stabilizing protein (AHSP) is believed to facilitate adult Hemoglobin A assembly and protect against toxic free α-globin subunits. Recombinant AHSP binds multiple forms of free α-globin to stabilize their structures and inhibit precipitation. However, AHSP also stimulates autooxidation of αO(2) subunit and its rapid conversion to a partially unfolded bishistidyl hemichrome structure. To investigate these biochemical properties, we altered the evolutionarily conserved AHSP proline 30 in recombinantly expressed proteins and introduced identical mutations into the endogenous murine Ahsp gene. In vitro, the P30W AHSP variant bound oxygenated α chains with 30-fold increased affinity. Both P30W and P30A mutant proteins also caused decreased rates of αO(2) autooxidation as compared with wild-type AHSP. Despite these abnormalities, mice harboring P30A or P30W Ahsp mutations exhibited no detectable defects in erythropoiesis at steady state or during induced stresses. Further biochemical studies revealed that the AHSP P30A and P30W substitutions had minimal effects on AHSP interactions with ferric α subunits. Together, our findings indicate that the ability of AHSP to stabilize nascent α chain folding intermediates prior to hemin reduction and incorporation into adult Hemoglobin A is physiologically more important than AHSP interactions with ferrous αO(2) subunits. 相似文献
147.
148.
149.
150.
C Miaskowski BA Cooper A Dhruva LB Dunn DJ Langford JK Cataldo CR Baggott JD Merriman M Dodd K Lee C West SM Paul BE Aouizerat 《PloS one》2012,7(7):e40560
The purposes of this study were to identify distinct latent classes of individuals based on subjective reports of sleep disturbance; to examine differences in demographic, clinical, and symptom characteristics between the latent classes; and to evaluate for variations in pro- and anti-inflammatory cytokine genes between the latent classes. Among 167 oncology outpatients with breast, prostate, lung, or brain cancer and 85 of their FCs, growth mixture modeling (GMM) was used to identify latent classes of individuals based on General Sleep Disturbance Scale (GSDS) obtained prior to, during, and for four months following completion of radiation therapy. Single nucleotide polymorphisms (SNPs) and haplotypes in candidate cytokine genes were interrogated for differences between the two latent classes. Multiple logistic regression was used to assess the effect of phenotypic and genotypic characteristics on GSDS group membership. Two latent classes were identified: lower sleep disturbance (88.5%) and higher sleep disturbance (11.5%). Participants who were younger and had a lower Karnofsky Performance status score were more likely to be in the higher sleep disturbance class. Variation in two cytokine genes (i.e., IL6, NFKB) predicted latent class membership. Evidence was found for latent classes with distinct sleep disturbance trajectories. Unique genetic markers in cytokine genes may partially explain the interindividual heterogeneity characterizing these trajectories. 相似文献