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M. J. Schwarz M. Super C. Wallis P. Beighton C. Newton L. E. Heptinstall C. Summers A. Markham G. Hambleton K. W. Webb D. Bilto D. Heaf M. Dalzell 《Human genetics》1990,85(4):428-430
Summary Details of haplotype and ΔF508 status from various populations represented in the cystic fibrosis (CF) DNA bank of the Royal
Manchester Children's Hospital are provided, together with information on the association of genotype and clinical status.
Clinical details and DNA analyses from native English in the North-West and South-West of England (Bath), from Lancashire
Pakistani families and from Afrikaans Namibian families are compared. A 78.5% incidence of ΔF508 has been found in English
families. Compound heterozygotes with CF and only one ΔF508 gene have an increased likelihood of having milder disease, with
lessPseudomonas isolated from sputum and relatively more showing either no regular respiratory pathogens or colonisation withStaphylococcus. There is also a relative increase in meconium ileus in these compound heterozygotes. The diagnosis of CF may be in doubt
in some subjects negative for ΔF508. Some of the Bath families have unusual haplotypes for an English population and a compound
heterozygote ΔF508/ΔI507 has been found. There is evidence from metD analysis of the founder effect in the Afrikaans Namibian
families, who have a high ΔF508 incidence. 相似文献
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To help define the immunological epitopes on human growth hormone (hGH), interaction of fragments of the hormone with 7 monoclonal antibodies (McAbs) was studied. Plasmin-digested hGH, containing two peptides (hGH1-134 and hGH141-191) joined by a disulphide bond, bound to each McAb with affinity similar to that of intact hGH. The purified C-terminal fragment, hGH141-191, showed low affinity for each McAb. The N-terminal fragment, hGH1-134, bound with quite high affinity to 2 McAbs (EB1 and EB3) but not to the other 5. We conclude that residues 1-134 of hGH contain the epitope to which McAbs EB1 and EB3 bind. 相似文献
166.
A C-terminal sequence from ox growth hormone 总被引:1,自引:0,他引:1
M Wallis 《Biochimica et biophysica acta》1966,115(2):423-428
167.
Direct stimulation of monocyte release of interleukin 1 by mycobacterial protein antigens 总被引:19,自引:0,他引:19
We examined stimulation of monocyte (MN) release of interleukin 1 (IL 1) by soluble microbial products. MN from tuberculin skin test nonreactive donors incubated with PPD (100 micrograms/ml) released IL 1 activity of 80.5 +/- 33.9 U/ml (mean +/- SD, n = 6), similar to that induced by optimal concentrations of LPS (76.4 U/ml). OKT3-reactive cells were not required for this process. PPD-stimulated IL 1 release by MN did not appear to be due to endotoxin contamination, as 1) PPD contained 0.01% endotoxin, 2) MN incubated in LPS (0.1 micrograms/ml) produced 19.5 +/- 13.9 U/ml, significantly less than PPD (p = 0.03), and 3) addition of polymyxin B (12.5 micrograms/ml) abrogated IL 1 production in response to LPS (0.1 microgram/ml) but had no significant effect on PPD induction of IL 1. Antigen 5, a partially purified cytoplasmic antigen of Mycobacterium tuberculosis, had similar IL 1-inducing effects. Arabinogalactan (a mycobacterial polysaccharide), streptolysin O, and tetanus toxoid did not. Thus, mycobacterial protein antigens directly stimulate MN to release IL 1. This property may be central to the response of the naive host to mycobacterial infection and may play a pathophysiologic role in tuberculosis. 相似文献
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Technical Considerations in the Preparation of Fluorescent-Antibody Conjugates 总被引:18,自引:9,他引:9
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Vester J. Lewis Wallis L. Jones John B. Brooks William B. Cherry 《Applied microbiology》1964,12(4):343-348
A comparison was made of (NH4)2SO4, HCl, ethodin, and ethanol for fractionation of rabbit antiserum prior to conjugation with fluorescein isothiocyanate. Fractionation with the salt was found to be the method of choice from the standpoints of simplicity and recovery of antibody effective in conjugates prepared from the fractions. Effects of pH, temperature, dye-protein ratio, and molarity and type of buffer upon conjugation were studied. These technical factors were adjusted to produce conjugates for Corynebacterium diphtheriae which possessed higher specific titers than did reagents obtained by previously employed techniques. 相似文献
170.