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71.
A second nonrandom translocation, der(16)t(1;16)(q21;q13), in Ewing sarcoma and peripheral neuroectodermal tumor 总被引:1,自引:0,他引:1
E C Douglass S T Rowe M Valentine D Parham W H Meyer E I Thompson 《Cytogenetics and cell genetics》1990,53(2-3):87-90
The majority of Ewing sarcomas and peripheral neuroectodermal tumors (PNET) that have been karyotyped contain a specific translocation, t(11;22)(q23;q11). We report here a second nonrandom translocation, der(16)t(1;16)(q21;q13), in 2 of 20 cases of Ewing sarcoma (seven previously unreported) and 2 of 7 cases of PNET (all previously unreported). All cases with this translocation also contained the t(11;22). Comparison of C-banding patterns in tumor and peripheral lymphocyte karyotypes in one case indicated that the likely breakpoints were 1q21 and 16q13. The presence of this translocation in cell lines will enable further investigation of the molecular events important in the pathogenesis of Ewing sarcoma and PNET. 相似文献
72.
Construction of a human chromosome 3 specific NotI linking library using a novel cloning procedure. 总被引:1,自引:0,他引:1
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E R Zabarovsky F Boldog T Thompson D Scanlon G Winberg Z Marcsek R Erlandsson E J Stanbridge G Klein J Sümegi 《Nucleic acids research》1990,18(21):6319-6324
Two new diphasmid vectors (lambda SK17 and SK22) and a novel procedure to construct linking libraries are described. A partial filling-in reaction provides counter-selection against false linking clones in the library, and obviates the need for supF selection. The diphasmid vectors, in combination with the novel selection procedure, have been used to construct a chromosome 3 specific NotI linking library from a human chromosome 3/mouse microcell hybrid cell line. The application of the new vectors and the strong biochemical and biological selections resulted in a library of 60,000 NotI linking clones. As practically all of them are real NotI linking clones (no false recombinants) the library represents approximately 3,000 human recombinants (equal to 10-15 genomic equivalents of chromosome 3). Previously published methods for construction of linking libraries are compared with the procedure described in the present paper. The advantages of the new vectors and the novel protocol are discussed. 相似文献
73.
We have analyzed changes in the structure of chloroplast chromosomes in response to light in growing Chlamydomonas cells using a crosslinking assay based on the intercalation of HMT (4'-hydroxymethyl-4,5',8-trimethylpsoralen) into DNA. Our results show that the structure of chloroplast chromosomes in at least three widely separated regions is different in light-grown vs. dark-grown cells. Structural changes in chloroplast chromosomes occur within 3 hrs after exposure to light or darkness, respectively. The response to light is not inhibited by atrazine and can be elicited by dim blue light incapable of evolving O2, indicating that it does not require photosynthesis. Inhibition of cytoplasmic protein synthesis with cycloheximide prevents this response to light, indicating that it depends, at least in part, on proteins imported from the cytoplasm. 相似文献
74.
Efficient site directed in vitro mutagenesis using ampicillin selection. 总被引:11,自引:1,他引:10
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A novel plasmid vector pSELECT-1 is described which can be used for highly efficient site-directed in vitro mutagenesis. The mutagenesis method is based on the use of single-stranded DNA and two primers, one mutagenic primer and a second correction primer which corrects a defect in the ampicillin resistance gene on the vector and reverts the vector to ampicillin resistance. Using T4 DNA polymerase and T4 DNA ligase the two primers are physically linked on the template. The non-mutant DNA strand is selected against by growth in the presence of ampicillin. In tests of the vector, highly efficient (60-90%) mutagenesis was obtained. 相似文献
75.
Karen J. Thompson 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1990,166(5):675-684
Summary Extracellular stimulation over the dorsal funiculus in the spinal cord of lampreys was found to selectively activate prolonged episodes of fictive arousal respiration (Figs. 1, 3). The induced episodes showed comparable increases in cycle frequency and motoneuron burst duration to the spontaneous arousal pattern observed in isolated brain preparations (Fig. 2). Intracellular stimulation of primary sensory neurons with axons in the dorsal funiculus, called dorsal cells, also elicited the arousal pattern (Fig. 4). Mechanoreceptive dorsal cells respond to cutaneous stimulation. When mechanical stimuli were applied to the skin of intact lampreys (Fig. 6) or to lampreys with ipsilateral vagotomy, arousal respiration was induced (Figs. 7, 8). Bilateral, but not unilateral, trigeminal lesion blocked dorsal cell induction of the arousal response (Fig. 5). Spontaneous arousal respiration was recorded from intact, unrestrained lampreys (Fig. 9). These results suggest that fictive arousal respiration is the in vitro correlate of natural arousal respiration in lampreys, and that one mechanism leading to arousal respiration may be the activity of sensory dorsal cells. A model for respiratory motor pattern switching in lamprey is proposed. The model suggests that the normal and arousal patterns are produced by separately engaging rostral or caudal pattern generators in the medulla, rather than by modifying one pattern generator (Fig. 10). 相似文献
76.
77.
Hair morphology was exammed on developing seedlings and mature plants of ten species from five of seven series inPhysalis. The taxonomic importance of hair development and the distribution of hair types then was evaluated with respect to other comparative data. Two classes of hair types were observed, as found in earlier investigations ofSolanum andRhododendron. The first hair class comprises a sequence from unbranched hairs to dendroid-stelliform hairs. Hairs of this class show considerable variability among taxa in size, presence or absence of glandular tips, and presence and degree of branching, and so were useful in making taxonomic comparisons. The second hair class consists of short hairs, each tipped with a multicellular gland. This second hair class was not useful taxonomically because of its lack of variability in morphology and its often rare occurrence on a plant. The taxa studied exhibit distinctive trichome features which serve to distinguish series and, in some cases together with other data, provided new insight into previously enigmatic species relationships. 相似文献
78.
P. Thompson 《Human Evolution》1990,5(2):133-138
The relevance of evolutionary theory to ethics goes back to Darwin but until recently discussion employed evolutionary theory
to justify ethical, social and political positions. Recently, evolutionary theory has been used to explain the existence of
moral systems and moral propensities and, thereby, to provide a naturalistic basis for ethics. I argue that this approach
has advanced our understanding of the basis of moral systems and moral propensities but does not as yet adequately incorporate
the role of cognition in its account. Cognition has the effect of decoupling to some extent — though, of course, far from
fully — human moral systems from their evolutionary origins. In an adequate account, evolutionary theory will play a crucial
role but so also will our evolved cognitive abilities. 相似文献
79.
M Bibbo D H Kim C di Loreto H E Dytch H Galera-Davidson D Thompson D L Richards H G Bartels P H Bartels 《Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology》1990,12(4):229-236
In research for the development of a computer-aided workstation for the objective grading of prostatic carcinoma, tissue architectural (histometric) features were analyzed in ten cases each of well-differentiated, moderately differentiated and poorly differentiated carcinoma (as subjectively graded by the consensus of a panel of experts). Sections were cut at 4 microns, stained by the Feulgen reaction and digitized by two different video-based photometric systems. Some images were interactively segmented, considering the histometric clues to be studied; others were automatically segmented by an expert system-guided technique. The latter procedure produced good results, with over 90% of the nuclei judged to be correctly segmented in 64% of the fields studied and over 80% in another 24% of the fields. While the number of nuclei per field provided some separation of well-differentiated from other lesions, the number of nuclei per gland distinguished between well-differentiated and moderately differentiated lesions. Simplicial decomposition of the images also provided a measure of the degree of differentiation, as did the "texture" of the nuclear placement, based on two run-length statistics. Combination of the run-length features distinguished the three categories of lesions with statistical significance. The results of this study provided insights into the problems (such as the effect of field boundaries) faced in the design of an computer-aided grading system. They also showed the value of expert system-guided scene segmentation and of such histometric features as the field cellularity and the number of nuclei per gland for the discrimination between lesions of different grades of differentiation. 相似文献
80.