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31.
To analyze transduction mechanisms in human lymphocyte killing, intracellular Ca2+ levels were increased by ionophore A23187 treatment and protein kinase C activated by phorbol ester 12-O-tetradecanoylphorbol-acetate (TPA). Drugs were tested either alone or in combinations on effector cells active in natural, antibody-dependent, and lectin-dependent killing. TPA suppressed killing in all systems at 100 ng/ml whereas A23187 was only suppressive for NK killing at concentrations higher than 0.1 microM. TPA combined with A23187, above 10 ng/ml and 0.5 microM, respectively, induced killing of all tested target cell lines with a slower kinetic than NK killing of K562 cells. Drug-induced killing did not increase optimal lectin and antibody-dependent killing and was demonstrated most easily on NK-resistant target cell lines. Fractionation of effector lymphocytes into NK cell-depleted, T3-positive and NK cell-enriched, T3-negative cells demonstrated that similar levels of TPA/A23187-dependent killing could be induced in both fractions. It is concluded that TPA/A23187 induce normal lymphocytes to nonselective killing of different target cells in similarity to the triggering effect these drugs have in many other cell systems. Whether the induced killing is representative of NK killing is discussed in relation to the presence of other potential effector cells and effector molecules in peripheral blood lymphocytes. 相似文献
32.
33.
Janet W. Reid 《Hydrobiologia》1987,153(2):121-138
Eight species of cyclopoid copepods were recorded from 1979–1983 in the complex microhabitats of a wet campo (campo úmido) marsh in central Brazil. Ectocyclops herbsti and Paracyclops fimbriatus occurred most often in areas with water covering the soil; Muscocyclops therasiae n. sp. occurred mainly in soils with no surface water; while Metacyclops campestris n. sp. showed no distinct microhabitat preference. Occurrence of the remaining four species was too sporadic to determine microhabitat preference. Paracyclops carectum n. sp., Metacyclops campestris n. sp., Muscocyclops therasiae n. sp., Muscocyclops bidentatus n. sp. and Ponticyclops boscoi n. g. n. sp. are described. A key to the New World species of Metacyclops s. str. is provided. 相似文献
34.
Batrachotoxin-modified sodium channels in planar lipid bilayers. Characterization of saxitoxin- and tetrodotoxin-induced channel closures 总被引:9,自引:7,他引:2
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The guanidinium toxin-induced inhibition of the current through voltage-dependent sodium channels was examined for batrachotoxin-modified channels incorporated into planar lipid bilayers that carry no net charge. To ascertain whether a net negative charge exists in the vicinity of the toxin-binding site, we studied the channel closures induced by tetrodotoxin (TTX) and saxitoxin (STX) over a wide range of [Na+]. These toxins carry charges of +1 and +2, respectively. The frequency and duration of the toxin-induced closures are voltage dependent. The voltage dependence was similar for STX and TTX, independent of [Na+], which indicates that the binding site is located superficially at the extracellular surface of the sodium channel. The toxin dissociation constant, KD, and the rate constant for the toxin-induced closures, kc, varied as a function of [Na+]. The Na+ dependence was larger for STX than for TTX. Similarly, the addition of tetraethylammonium (TEA+) or Zn++ increased KD and decreased kc more for STX than for TTX. These differential effects are interpreted to arise from changes in the electrostatic potential near the toxin-binding site. The charges giving rise to this potential must reside on the channel since the bilayers had no net charge. The Na+ dependence of the ratios KDSTX/KDTTX and kcSTX/kcTTX was used to estimate an apparent charge density near the toxin-binding site of about -0.33 e X nm-2. Zn++ causes a voltage-dependent block of the single-channel current, as if Zn++ bound at a site within the permeation path, thereby blocking Na+ movement. There was no measurable interaction between Zn++ at its blocking site and STX or TTX at their binding site, which suggests that the toxin-binding site is separate from the channel entrance. The separation between the toxin-binding site and the Zn++ blocking site was estimated to be at least 1.5 nm. A model for toxin-induced channel closures is proposed, based on conformational changes in the channel subsequent to toxin binding. 相似文献
35.
To investigate aspects of the biochemical nature of membrane-bound dopamine D1 receptors, rat striatal homogenates were pretreated with heavy metal cations and some other chemical agents, and their effects on D1 receptors were subsequently determined using a standard [3H](R)-(+)-8-chloro-2,3,4,5-tetrahydro-3-methyl-5-phenyl-1-N-3- benzazepine([3H]SCH 23390) binding assay. Incubation of striatal membranes with as little as 1 microM Hg2+, 10 microM Cu2+, and 10 microM Cd2+ completely prevented specific [3H]SCH 23390 binding. The effect of Cu2+, 1.5 microM, was noncompetitive in nature, whereas 3-5 microM Cu2+ afforded mixed-type inhibition. The inhibitory effect of Cu2+ was fully reversed by dithiothreitol (0.1-1 mM). Cu2+ (2 microM) did not affect the affinity of cis-flupenthixol or clozapine for remaining [3H]SCH 23390 sites. A second series of cations, Co2+ (30 microM), Ni2+ (30 microM), Mn2+ (1 mM), Ca2+ (25 mM), and Ba2+ (20 mM), inhibited specific [3H]SCH 23390 binding by 50% at the concentrations indicated. The thiol alkylating reagent N-ethylmaleimide (NEM) (0.2 mM) reduced specific binding by 70%. The effect of NEM was completely prevented by coincubation with a D1 receptor saturating concentration of SCH 23390 (20 nM) or dopamine (10 microM). The results indicated that the dopamine D1 receptor is a thiol protein and that a thiol group is essential for the ligand binding. 相似文献
36.
M B Elam E S Umstot R N Andersen S S Solomon M Heimberg 《Biochimica et biophysica acta》1987,921(3):531-540
Given the same quantity of fatty acid, livers from male rats esterify less fatty acid and secrete less triacylglycerol in very-low-density lipoprotein than do livers from female animals. To elucidate the role of testosterone in maintenance of this male pattern, conversion of [1-14C]oleic acid into triacylglycerol was assessed in vitro by rat hepatocytes (male) following gonadectomy and replacement with testosterone. Following castration, incorporation of fatty acid into triacylglycerol was increased. In contrast, esterification of exogenous fatty acid into phospholipid, cholesteryl esters, and diacylglycerol was unchanged. Treatment with testosterone (75 micrograms/day) reduced incorporation of exogenous fatty acid into triacylglycerol. Higher doses of testosterone (200 or 100 micrograms/day) modified the effect, such that inhibition was observed only at low oleate (0.5 mM) concentrations. At higher substrate concentrations (1.0-2.0 mM) the inhibitory effect was no longer observed. Further, a similar dose-dependent effect of testosterone was observed following in vivo treatment of castrate females with testosterone. These data support the concept of a regulatory role of testosterone in hepatic triacylglycerol synthesis. These findings also demonstrate a biphasic effect of testosterone, an effect that is dependent not only upon the dose of testosterone administered, but also on the concentration of fatty acid to which the hepatocyte is exposed in vitro. 相似文献
37.
The isolation and characterization of RNA coded by the micF gene in Escherichia coli. 总被引:9,自引:0,他引:9
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J Andersen N Delihas K Ikenaka P J Green O Pines O Ilercil M Inouye 《Nucleic acids research》1987,15(5):2089-2101
38.
Alan N. Andersen 《Oecologia》1989,81(3):310-315
Summary The many evolutionary modifications to seed biology in response to seed predation do not necessarily imply that seed predators have an important impact on population recruitment. This is because competition between individual plants for rare safe sites can cause an oversupply of seeds so far as a population is concerned. The importance of seed losses to population recruitment at any point in time is related to the abundance of safe sites (Fig. 1): it is zero when safe sites are absent, negligible when safe sites are rare, and greatest when safe sites are numerous enough for recruitment to be limited by seed supply. Here I interpret the impact of severe seed losses on population recruitment in four species of long-lived perennials (Eucalyptus baxteri, Leptospermum juniperinum, Casuarina pusilla and L. myrsinoides) by considering these losses in terms of the overall seed dynamics of the populations. I focus on seed supply and seedling survival, as a measure of the current abundance of safe sites, and the maintenance of seed banks, as a measure of the ability of populations to exploit any future changes in safe site abundance. Insect seed predators destroyed about 95% of total seeds in each case. However, these losses do not necessarily have an important impact on population recruitment, because: (i) in most years recruitment appears to be limited by a rarity of safe sites, and not by seed supply (which was as high as 43 germinable seeds/m2/yr); and (ii) the losses did not prevent the establishment of large seed banks (ranging from >30 to >1000 viable seeds/m2) potentially capable of exploiting temporary conditions favourable for recruitment. In contrast to the situation with many annual plants, patterns of recruitment in stable populations of long-lived perennials are often extremely complex, and the significance of seed losses therefore difficult to determine. 相似文献
39.
Strains of Vibrio salmonicida isolated from Atlantic salmon (Salmo salar) and rainbow trout (Salmo gairdneri) suffering from cold-water vibriosis could be divided on the basis of plasmid profiles into four different categories. Of 32 strains, 19% harbored three plasmids of 24, 3.4, and 26 megadaltons (MDa), 69% harbored the 24- and 3.4-MDa plasmids but not the 2.6-MDA plasmid, and 9% harbored only the 24-MDA plasmid. The fourth category, which consisted of only one strain, harbored a plasmid of 10 MDa. In spite of different plasmid patterns, the strains of V. salmonicida were very similar with respect to biochemical reactions. The one-third of the V. salmonicida strains which were serotyped were of the same type. The 50% lethal doses, which were determined by intraperitoneal injection, ranged from 4 x 106 to 1 x 108 CFU per fish. 相似文献
40.
Rose C. Wade Janet V. Powers Cyril Ponnamperuma 《Origins of life and evolution of the biosphere》1989,19(2):199-220
This bibliography is the seventeenth annual supplement to the comprehensive bibliography on the same subject which was published in Space Life Sci. 2(1970), 225-295; 3(1972), 293-304; 4(1973), 309-329 and in Origins of Life 5(1974), 505-527; 6(1975), 285-300; 7(1976), 75-85; 8(1977), 59-66; 9(1978), 67-74; 10(1980), 69-87; 10(1980), 379-404; 11(1981), 273-288; 12(1982), 93-118; 13(1983), 61-80; 15(1984), 55-69; 17(1987), 171-184; 17(1987), 185-206. 相似文献