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261.
The prokaryotic endosymbionts that became plastids and mitochondria contained genes destined for one of three fates. Genes
required for free-living existence were lost. Most genes useful to the symbiosis were transferred to the nucleus of the host.
Some genes, a small minority, were retained within the organelle. Here we suggest that a selective advantage of movement of
genes to the nucleus is decreased mutation: plastids and mitochondria have high volume-specific rates of redox reactions,
producing oxygen free radicals that chemically modify DNA. These mutations lead to synthesis of modified electron carriers
that in turn generate more mutagenic free radicals—the “vicious circle” theory of aging. Transfer of genes to the nucleus
is also advantageous in facilitating sexual recombination and DNA repair. For genes encoding certain key components of photosynthesis
and respiration, direct control of gene expression by redox state of electron carriers may be required to minimize free radical
production, providing a selective advantage of organelle location which outweighs that of location in the nucleus. A previous
proposal for transfer of genes to the nucleus is an economy of resources in having a single genome and a single apparatus
for gene expression, but this argument fails if any organellar gene is retained. A previous proposal for the retention of
genes within organelles is that certain proteins are organelle-encoded because they cannot be imported, but there is now evidence
against this view. Decreased free radical mutagenesis and increased sexual recombination upon transfer to the nucleus together
with redox control of gene expression in organelles may now account for the slightly different gene distributions among nuclei,
plastids, and mitochondria found in major eukaryote taxa. This analysis suggests a novel reason for uniparental inheritance
of organelles and the evolution of anisogametic sex, and may also account for the occurrence of nitrogen fixation in symbionts
rather than in nitrogen-fixing organelles.
Correspondence to: J.F. Allen 相似文献
262.
263.
Carboxylation of epoxides to beta-keto acids in cell extracts of Xanthobacter strain Py2. 总被引:3,自引:3,他引:0 下载免费PDF全文
A novel enzymatic reaction involved in the metabolism of aliphatic epoxides by Xanthobacter strain Py2 is described. Cell extracts catalyzed the CO2-dependent carboxylation of propylene oxide (epoxypropane) to form acetoacetate and beta-hydroxybutyrate. The time courses of acetoacetate and beta-hydroxybutyrate formaton indicate that acetoacetate is the primary product of propylene oxide carboxylation and that beta-hydroxybutyrate is a secondary product formed by the reduction of acetoacetate. Analogous C5 carboxylation products were identified with 1,2-epoxybutane as the substrate. In the absence of CO2, propylene oxide and 1,2-epoxybutane were isomerized to form acetone and methyl ethyl ketone, respectively, as dead-end products. The carboxylation of short-chain epoxides to beta-keto acids is proposed to serve as the physiological reaction for the metabolism of aliphatic epoxides in Xanthobacter strain Py2. 相似文献
264.
The Saccharomyces cerevisiae MEC1 gene, which encodes a homolog of the human ATM gene product, is required for G1 arrest following radiation treatment. 总被引:2,自引:0,他引:2 下载免费PDF全文
The Saccharomyces cerevisiae gene MEC1 represents a structural homolog of the human gene ATM mutated in ataxia telangiectasia patients. Like human ataxia telangiectasia cell lines, mec1 mutants are defective in G2 and S-phase cell cycle checkpoints in response to radiation treatment. Here we show an additional defect in G1 arrest following treatment with UV light or gamma rays and map a defective arrest stage at or upstream of START in the yeast cell cycle. 相似文献
265.
Knockout of the two ldh genes has a major impact on peptidoglycan precursor synthesis in Lactobacillus plantarum. 总被引:3,自引:2,他引:1 下载免费PDF全文
T Ferain J N Hobbs Jr J Richardson N Bernard D Garmyn P Hols N E Allen J Delcour 《Journal of bacteriology》1996,178(18):5431-5437
Most bacteria synthesize muramyl-pentapeptide peptidoglycan precursors ending with a D-alanyl residue (e.g., UDP-N-acetylmuramyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala). However, it was recently demonstrated that other types of precursors, notably D-lactate-ending molecules, could be synthesized by several lactic acid bacteria. This particular feature leads to vancomycin resistance. Vancomycin is a glycopeptide antibiotic that blocks cell wall synthesis by the formation of a complex with the extremity of peptidoglycan precursors. Substitution of the terminal D-alanine by D-lactate reduces the affinity of the antibiotic for its target. Lactobacillus plantarum is a lactic acid bacterium naturally resistant to vancomycin. It converts most of the glycolytic pyruvate to L- and D-lactate by using stereospecific enzymes designated L- and D-lactate dehydrogenases, respectively. In the present study, we show that L. plantarum actually synthesizes D-lactate-ending peptidoglycan precursors. We also report the construction of a strain which is deficient for both D- and L-lactate dehydrogenase activities and which produces only trace amounts of D- and L-lactate. As a consequence, the peptidoglycan synthesis pathway is drastically affected. The wild-type precursor is still present, but a new type of D-alanine-ending precursor is also synthesized in large quantities, which results in a highly enhanced sensitivity to vancomycin. 相似文献
266.
The impact on reproductive success of investigators studying the breeding biology of royal and rockhopper penguins was assessed.
Control and experimental transects were established in a colony of each species and the number of active nests, from egg laying
to creche stage, were compared. Experimental nests were those used in breeding biology work, where birds were measured and
banded, and nest checks were carried out at least once per week. Control nests were in equivalent locations but birds were
not handled, and no contact was made with the nests once breeding had begun. There were no significant differences in the
number of active nests between the control and experimental transects (and, therefore, breeding success) in either species.
It is concluded that, provided care is taken when working with these species, no impacts on the short-term (up to creche stage,
in one breeding season) breeding success of these populations will occur. 相似文献
267.
Phosphorylation of cardiac junctional and free sarcoplasmic reticulum (SR) by protein kinase C (PKC) isoforms and was investigated. Both SR and PKC were isolated from canine heart. Junctional and free SR vesicles were prepared by calcium-phosphate-loading. The substrate specificities of PKC and PKC were found to be similar in both SR fractions. A high molecular weight junctionally-associated protein was phosphorylated by PKA, PKC and an endogenous Ca2+/calmodulin-dependent protein kinase activity: the highest levels of phosphate incorporation being catalysed by the latter kinase. In addition to this high molecular weight junctionally-associated protein, PKC induced phosphorylation of 45, 96 kDa and several proteins of greater than 200 kDa in junctional SR. A protein of 96 kDa was phosphorylated by both isoforms in junctional and free SR. The major substrate for PKA, PKC, PKC and the Ca2+/calmodulin-dependent protein kinase, in both junctional and free SR, was phospholamban. Although the phosphorylation of phospholamban by PKC was activated by Ca2+, a component of this activity appeared to be independent of Ca2+. PKC-mediated phosphorylation of phospholamban was fully activated by 1 M Ca2+ whereas the Ca2+/calmodulin dependent kinase required concentrations in excess of 5 M Ca2+. In the in vitro system employed in these studies, the concentrations of either PKC or the catalytic subunit of PKA required to phosphorylate phospholamban were found to be similar. In addition, in the presence of a 15 kDa sarcolemmal-associated protein, which becomes phosphorylated upon activation of PKC in vivo, phosphorylation of phospholamban by PKC was unaffected. These results demonstrate that, although substrates for both subtypes are found in both junctional and free SR, PKC and PKC do not show differences in selectivity towards these substrates.Abbreviations Ca2+
free calcium
- CaM kinase
Ca2+/calmodulin-dependent protein kinase
- DTT
dithiothreitol
- EDTA
ethylenediaminetetraacetic acid
- EGTA
ethylene glycol bis(b-aminoethylether)-N,N,N,N-tetraacetic acid
- FSR
free sarcoplasmic reticulum
- JSR
junctional sarcoplasmic reticulum
- PKC
protein kinase C
- PS
phosphatidylserine
- SDS
sodium dodecyl sulfate
- SAG
1-stearoyl-2-arachidonylglycerol
- TPCK
L-1-tosylamido-2-phenylethyl chloromethyl ketone
- Tris/HCI
tris(hydroxymethyl)aminomethane hydrochloride
This work was supported by a grant (to S.K.) from the Heart and Stroke Foundation of B.C. and Yukon. The costs of publication of this article were defrayed in part by the payment of page charges This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.Recipient of a Studentship form the Heart and Stroke Foundation of Canada. 相似文献
268.
Xóchttl Castañeda Víctor Ortíz Betania Allen Cecilia García Mauricio Hernández-Avila 《Culture, medicine and psychiatry》1996,20(2):229-247
The central topic of the article is the divided world of female commercial sex workers (FCSW) in Mexico City. Fourteen focus group sessions were conducted with 133 FCSW from varying socio-economic levels and types of work site, as well as seven individual interviews.FCSW live in a constant double bind, as mother and prostitute, and come into daily contact with society's double standard for women. Reactions include justifying sex work as a better paying employment opportunity for women, as a necessary evil, and as a type of social service, while at the same time hiding their profession from their families. FCSW also live out an archetypal female ambivalence, their selves divided between the mother/saint and the traitor/prostitute.This article defines elements which should be taken into account in culturally appropriate programs for prevention of HIV/AIDS transmission, especially the importance which FCSW give to their role as mothers and promotion of the condom as a physical and symbolic barrier between professional and private life. 相似文献
269.
Charles P. Moehs Paul V. Allen Mendel Friedman William R. Belknap 《Plant molecular biology》1996,32(3):447-452
We have isolated a cDNA encoding transaldolase, an enzyme of the pentose-phosphate pathway, from potato (Solanum tuberosum). The 1.5 kb cDNA encodes a protein of 438 amino acid residues with a molecular mass of 47.8 kDa. When the potato cDNA was expressed in Escherichia coli a 45 kDa protein with transaldolase activity was produced. The first 62 amino acids of the deduced amino acid sequence represent an apparent plastid transit sequence. While the potato transaldolase has considerable similarity to the enzyme from cyanobacteria and Mycobacterium leprae, similarity to the conserved transaldolase enzymes from humans, E. coli and Saccharomyces cerevisiae is more limited. Northern analysis indicated that the transaldolase mRNA accumulated in tubers in response to wounding. Probing the RNA from various potato tissues indicated that the transaldolase mRNA accumulation to higher levels in the stem of mature potato plants than in either leaves or tubers. These data are consistent with a role for this enzyme in lignin biosynthesis. 相似文献
270.
The survival of unfed males and females of six species of African ticks was monitored at five different saturation deficits at constant temperature (25°C). The survivorship curves for each species comprised a pre-mortality period, prior to when ticks started to die and a mortality period corresponding to a rapid increase in the mortality rate. Longevity was defined as pre-mortality plus mortality. A negative correlation between the longevity of the ticks and the saturation deficits was found with ticks surviving longer at lower deficits. The survival of males and females was similar. At low saturation deficits (2–4 mmHg) Amblyomma hebraeum survived the longest periods (74 weeks). Some correlation was found between the tick survival under dehydrating conditions and habitat associations. Rhipicephalus appendiculatus and Haemaphysalis leachii, the most mesic in distribution, had the shortest longevity (21 and 13 weeks, respectively) at high saturation deficits (7–21 mmHg). Hyalomma marginatum rufipes, the most xerophilic in distribution, had the longest survival (39.3±10.5 weeks) at high saturation deficits. Other factors apart from the adult survival should be taken into account when accounting for the tick distribution, in particular the tolerance of earlier developmental stages to desiccation. 相似文献