全文获取类型
收费全文 | 122篇 |
免费 | 10篇 |
出版年
2024年 | 1篇 |
2023年 | 2篇 |
2022年 | 3篇 |
2021年 | 2篇 |
2019年 | 1篇 |
2018年 | 1篇 |
2016年 | 4篇 |
2015年 | 3篇 |
2014年 | 7篇 |
2013年 | 6篇 |
2012年 | 8篇 |
2011年 | 6篇 |
2010年 | 4篇 |
2009年 | 5篇 |
2008年 | 6篇 |
2007年 | 8篇 |
2006年 | 8篇 |
2005年 | 4篇 |
2004年 | 5篇 |
2003年 | 7篇 |
2002年 | 5篇 |
2001年 | 2篇 |
2000年 | 2篇 |
1999年 | 2篇 |
1998年 | 6篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1995年 | 2篇 |
1994年 | 2篇 |
1993年 | 3篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1980年 | 1篇 |
1978年 | 1篇 |
1973年 | 2篇 |
1966年 | 1篇 |
排序方式: 共有132条查询结果,搜索用时 15 毫秒
71.
Andreas Jenner Aida PeaBlanco Raquel SalvadorGallego Begoa UgarteUribe Cristiana Zollo Tariq Ganief Jan Bierlmeier Markus Mund Jason E Lee Jonas Ries Dirk Schwarzer Boris Macek Ana J GarciaSaez 《The EMBO journal》2022,41(8)
The apoptotic executioner protein BAX and the dynamin‐like protein DRP1 co‐localize at mitochondria during apoptosis to mediate mitochondrial permeabilization and fragmentation. However, the molecular basis and functional consequences of this interplay remain unknown. Here, we show that BAX and DRP1 physically interact, and that this interaction is enhanced during apoptosis. Complex formation between BAX and DRP1 occurs exclusively in the membrane environment and requires the BAX N‐terminal region, but also involves several other BAX surfaces. Furthermore, the association between BAX and DRP1 enhances the membrane activity of both proteins. Forced dimerization of BAX and DRP1 triggers their activation and translocation to mitochondria, where they induce mitochondrial remodeling and permeabilization to cause apoptosis even in the absence of apoptotic triggers. Based on this, we propose that DRP1 can promote apoptosis by acting as noncanonical direct activator of BAX through physical contacts with its N‐terminal region. 相似文献
72.
Functional coexpression of serine protein kinase SRPK1 and its substrate ASF/SF2 in Escherichia coli
Bai-Gong Yue Paul Ajuh Gran Akusjrvi Angus I. Lamond Jan-Peter Kreivi 《Nucleic acids research》2000,28(5):e14
Mammalian proteins expressed in Escherichia coli are used in a variety of applications. A major drawback in producing eukaryotic proteins in E.coli is that the bacteria lack most eukaryotic post-translational modification systems, including serine/threonine protein kinase(s). Here we show that a eukaryotic protein can be phosphorylated in E.coli by simultaneous expression of a mammalian protein kinase and its substrate. We show that in bacteria expressing SRPK1, ASF/SF2 becomes phosphorylated to a degree resembling native ASF/SF2 present in interphase HeLa cell nuclei. The E.coli phosphorylated ASF/SF2 is functional in splicing and, contrary to the unphosphorylated protein, soluble under native conditions. 相似文献
73.
74.
pBINPLUS: An improved plant transformation vector based on pBIN19 总被引:34,自引:0,他引:34
Fred A. van Engelen Jos W. Molthoff Anthony J. Conner Jan-Peter Nap Andy Pereira Willem J. Stiekema 《Transgenic research》1995,4(4):288-290
We describe the construction of a new plant transformation vector, pBINPLUS, based on the popular pBIN19 vector. Improvements over pBIN19 include location of the selectable marker gene at the left T-DNA border, a higher copy number inE. coli, and two rare restriction sites around the multiple cloning site for easier cloning and analysis of T-DNA insertions in plant genomes. 相似文献
75.
Biosafety of kanamycin-resistant transgenic plants 总被引:9,自引:0,他引:9
Kanamycin resistance is one of the most frequently used selection markers for obtaining transgenic plants. The introduction
of these transgenic plants into agricultural practice will cause the kanamycin resistance gene and the gene product to be
present on a large scale. The desirability of this situation is analysed. The nature, properties and applications of the antibiotic
kanamycin are briefly reviewed, as are the mechanisms of kanamycin resistance. It is argued that the gene used for resistance
is an excellent choice because of the high substrate specificity of the enzyme encoded. Human or veterinary antibiotic therapies
will not be compromised. Also, the physico-chemical characteristics of the antibiotic exclude the existence of selective conditions
in the environment. Therefore, a transgenic plant or any other organism that might have acquired the gene will not get any
selective advantage because of this gene. Evidence further suggests there is no toxicity or predictable harm of both gene
or gene product for human or animal consumption. Full legislative clearance of this transgenic trait is therefore acceptable. 相似文献
76.
Matthias Roth-Kleiner Thomas M. Berger Sandrine Gremlich Stefan A. Tschanz Sonja I. Mund Martin Post Marco Stampanoni Johannes C. Schittny 《Histochemistry and cell biology》2014,141(1):75-84
Pre- and postnatal corticosteroids are often used in perinatal medicine to improve pulmonary function in preterm infants. To mimic this clinical situation, newborn rats were treated systemically with dexamethasone (Dex), 0.1–0.01 mg/kg/day on days P1–P4. We hypothesized that postnatal Dex may have an impact on alveolarization by interfering with extracellular matrix proteins and cellular differentiation. Morphological alterations were observed on 3D images obtained by high-resolution synchrotron radiation X-ray tomographic microscopy. Alveolarization was quantified stereologically by estimating the formation of new septa between days P4 and P60. The parenchymal expression of tenascin-C (TNC), smooth muscle actin (SMA), and elastin was measured by immunofluorescence and gene expression for TNC by qRT-PCR. After Dex treatment, the first phase of alveolarization was significantly delayed between days P6 and P10, whereas the second phase was accelerated. Elastin and SMA expressions were delayed by Dex treatment, whereas TNC expression was delayed and prolonged. A short course of neonatal steroids impairs the first phase of alveolarization, most likely by altering the TNC and elastin expression. Due to an overshooting catch-up during the second phase of alveolarization, the differences disappear when the animals reach adulthood. 相似文献
77.
78.
Jan-Peter Frahm 《Biodiversity and Conservation》2008,17(2):277-284
Bryophytes disperse by small unicellular spores between 7 μm und usually less than 100 μm. A large percentage of species is
sterile and propagates vegetatively either by special brood bodies or fragments of whole plants. It is shown that there is
no difference in the effectiveness between generative and vegetative propagation. Size and weight of the diaspores suggest
that both must easily be dispersed and the species must therefore have wide ranges. This does, however, not result in ubiquitous
occurrence. This is only true for part of the species. Many, even sterile species show wide transcontinental ranges. On the
other hand, there are many examples of limited to very limited distribution in spite of a rich production of diaspores. These
are explained by narrow ecological niches, age of taxa, local extinction or historical events such as ice ages. Conspicuously,
species can loose the ability for dispersal for unknown (perhaps genetic) reasons, which may ultimately lead to extinction.
Special Issue: Protist diversity and geographic distribution. Guest editor: W. Foissner. 相似文献
79.
Directed microspore-specific recombination of transgenic alleles to prevent pollen-mediated transmission of transgenes 总被引:6,自引:0,他引:6
A major challenge for future genetically modified (GM) crops is to prevent undesired gene flow of transgenes to plant material intended for another use. Recombinase-mediated auto excision of transgenes directed by a tightly controlled microspore-specific promoter allows efficient removal of either the selectable marker gene or of all introduced transgenes during microsporogenesis. This way, transgene removal becomes an integral part of the biology of pollen maturation, not requiring any external stimulus such as chemical induction by spraying. We here show the feasibility of engineering transgenic plants to produce pollen devoid of any transgene. Highly efficient excision of transgenes from tobacco pollen was achieved with a potential failure rate of at most two out of 16 800 seeds (0.024%). No evidence for either premature activation or absence of activation of the recombinase system was observed under stress conditions in the laboratory. This approach can prevent adventitious presence of transgenes in non-GM crops or related wild species by gene flow. Such biological containment may help the deployment and management of coexistence practices to support consumer choice and will promote clean molecular farming for the production of high-value compounds in plants. 相似文献
80.