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71.
Anne-Claire van Altvorst Herma Koehorst Jan de Jong Hans J. M. Dons 《Plant Cell, Tissue and Organ Culture》1996,45(2):169-173
Transgenic carnation plants were obtained after infection of petal explants with the supervirulent Agrobacterium tumefaciens strain AGLO. Southern blot techniques confirmed the transgenic nature of four transformed plants. The expression of the gus gene was verified in these plants by histochemical assays on selected shoots. It was very difficult to transfer the transgenic plants to the greenhouse due to vitrification and premature flowering.Abbreviations BA
6-benzyladenine
- GA3
gibberellic acid
-
gus-
glucuronidase gene
- MS
Murashige and Skoog (1962)
- NAA
naphthaleneacetic acid
-
nptII
neomycin phosphotransferase II gene
- PCR
polymerase chain reaction 相似文献
72.
Leon P.M. Lamers G. Els Ten Dolle Serge T.G. Van Den Berg Sebastiaan P.J. Van Delft Jan G.M. Roelofs 《Biogeochemistry》2001,55(1):87-101
Sulphate (SO4
2-)reduction rates are generally low in freshwaterwetlands and are regulated by the scarceavailability of the ion. Increasedconcentrations of this electron acceptor due tosulphur (S) pollution of groundwater andsurface water may, however, lead to highSO4
2- reduction rates now regulatedby the availability of appropriate electrondonors. Due to variations in this availability,the response to S pollution (e.g. from surfacewater or groundwater) is expected to differbetween soils. This hypothesis was tested inlaboratory mesocosm experiments by comparingtwo wetland soil types with distinctlydifferent humus profiles: a Hydromoder and aRhizomull type. In the first type, expected tohave a higher availability of degradable soilorganic matter (SOM), SO4
2-availability appeared to be rate limiting forSO4
2- reduction. In the Rhizomullsoils, in contrast, the electron acceptor didnot limit SO4
2- reduction rates athigher concentrations. These differences inresponse could not, however, be attributed todifferences in the various SOM fractions or inSOM densities. Eutrophication and free sulphideaccumulation, two major biogeochemical problemscaused by SO4
2- pollution, occurredin both types. The absolute extent ofphosphorus mobilisation was determined by theconcentration of this element in the soil (C/Pratio), while the level of sulphideaccumulation was governed by the concentrationof dissolved iron in the pore water. It wastherefore concluded that neither the humusprofile nor the concentrations of different SOMfractions in the soils are reliable indicatorsfor the sensitivity of wetland types to Spollution. 相似文献
73.
Development of a 16S rRNA gene-based prototype microarray for the detection of selected actinomycetes genera 总被引:1,自引:0,他引:1
Kyselková M Kopecký J Felföldi T Cermák L Omelka M Grundmann GL Moënne-Loccoz Y Ságová-Marecková M 《Antonie van Leeuwenhoek》2008,94(3):439-453
Actinomycetes are known for their secondary metabolites, which have been successfully used as drugs in human and veterinary medicines. However, information on the distribution of this group of Gram-positive bacteria in diverse ecosystems and a comprehension of their activities in ecosystem processes are still scarce. We have developed a 16S rRNA-based taxonomic microarray that targets key actinomycetes at the genus level. In total, 113 actinomycete 16S rRNA probes, corresponding to 55 of the 202 described genera, were designed. The microarray accuracy was evaluated by comparing signal intensities with probe/target-weighted mismatch values and the Gibbs energy of the probe/target duplex formation by hybridizing 17 non-actinomycete and 29 actinomycete strains/clones with the probe set. The validation proved that the probe set was specific, with only 1.3% of false results. The incomplete coverage of actinomycetes by a genus-specific probe was caused by the limited number of 16S rRNA gene sequences in databases or insufficient 16S rRNA gene polymorphism. The microarray enabled discrimination between actinomycete communities from three forest soil samples collected at one site. Cloning and sequencing of 16S rRNA genes from one of the soil samples confirmed the microarray results. We propose that this newly constructed microarray will be a valuable tool for genus-level comparisons of actinomycete communities in various ecological conditions. 相似文献
74.
Our knowledge of recombination rates and patterns in plants is far from being comprehensive. However, compelling evidence indicates a central role for recombination, through its influences on mutation and selection, in the evolution of plant genomes. Furthermore, recombination seems to be generally higher and more variable in plants than in animals, which could be one of the primary reasons for differences in genome lability between these two kingdoms. Much additional study of recombination in plants is needed to investigate these ideas further. 相似文献
75.
76.
We introduce a novel computational approach to predict effective genome size (EGS; a measure that includes multiple plasmid copies, inserted sequences, and associated phages and viruses) from short sequencing reads of environmental genomics (or metagenomics) projects. We observe considerable EGS differences between environments and link this with ecologic complexity as well as species composition (for instance, the presence of eukaryotes). For example, we estimate EGS in a complex, organism-dense farm soil sample at about 6.3 megabases (Mb) whereas that of the bacteria therein is only 4.7 Mb; for bacteria in a nutrient-poor, organism-sparse ocean surface water sample, EGS is as low as 1.6 Mb. The method also permits evaluation of completion status and assembly bias in single-genome sequencing projects. 相似文献
77.
Maize callus cells possess numerous protein bodies which develop as sub-compartments of the endoplasmic reticulum. We localized
maize calreticulin mRNAs and protein in maize callus cells using in situ hybridization and immunocytochemistry. Calreticulin
mRNAs were selectively targeted to the endoplasmic reticulum (ER) subdomains surrounding protein bodies. Profilin mRNAs, used
as a positive control for in situ hybridization experiments, showed distinct and rather diffuse localization pattern. Using
both, immunofluorescence and immunogold electron microscopy localization techniques, calreticulin was found to be enriched
around and within protein bodies in maize callus storage cells. As a positive control for reticuloplasmins, HDEL antibody
revealed labelling of protein bodies and of the nuclear envelope. The identity of protein bodies was confirmed by specific
binding of an α zein antibody. These data suggest that calreticulin mRNA is targeted towards protein body forming subdomains
of the ER, and that calreticulin is localized and enriched in these protein bodies. The possibility that calreticulin plays
an important role in zein retention within the ER and/or its assembly and packaging into protein bodies during protein body
biogenesis in maize callus is discussed. 相似文献
78.
Seedlings of two barley genotypes (‘Maresi’ and wild form of Hordeum spontaneum) were treated with jasmonic acid (JA 5 μM and 15 μM) for 24 h, and then subjected to water stress (PEG 6000 solution of −
1.5 MPa). JA caused an increase in the content of ABA but not in that of proline and spermidine in the two studied genotypes.
The effect of the treatment did not depend on the applied JA concentration. The pre-stress treatment with JA changed plant
response to water deficit with regard to membrane injury. Treatment with a lower JA concentration (5 μM) caused a substantial
reduction of the stress-induced membrane damage in the both genotypes. A higher JA concentration (15 μM) caused the reduction
of membrane injury only in H. spontaneum and was ineffective in ‘Maresi’. JA had no influence on the leaf water status in water-stressed plants. A possible role of
JA in leaf ABA accumulation and alleviation of cell membrane injury under water deficit is discussed.
The work was partly supported by the Polish Committee For Scientific Research, grant No 5 PO6A 036 18 相似文献
79.
Elke EM Brouwers Alwin DR Huitema Jos H Beijnen Jan HM Schellens 《BMC clinical pharmacology》2008,8(1):7
Background
The aim of this study was to evaluate long-term platinum retention in patients treated with cisplatin and oxaliplatin. 相似文献80.
Sophie-Marie Aicher Felix Streicher Maxime Chazal Delphine Planas Dongsheng Luo Julian Buchrieser Monika Nemcova Veronika Seidlova Jan Zukal Jordi Serra-Cobo Dominique Pontier Bertrand Pain Gert Zimmer Olivier Schwartz Philippe Roingeard Jiri Pikula Laurent Dacheux Nolwenn Jouvenet 《Journal of virology》2022,96(14)