全文获取类型
收费全文 | 25024篇 |
免费 | 2175篇 |
国内免费 | 13篇 |
专业分类
27212篇 |
出版年
2023年 | 92篇 |
2022年 | 220篇 |
2021年 | 412篇 |
2020年 | 253篇 |
2019年 | 343篇 |
2018年 | 434篇 |
2017年 | 388篇 |
2016年 | 632篇 |
2015年 | 1075篇 |
2014年 | 1204篇 |
2013年 | 1508篇 |
2012年 | 1727篇 |
2011年 | 1646篇 |
2010年 | 1083篇 |
2009年 | 986篇 |
2008年 | 1299篇 |
2007年 | 1207篇 |
2006年 | 1248篇 |
2005年 | 1188篇 |
2004年 | 1121篇 |
2003年 | 968篇 |
2002年 | 1078篇 |
2001年 | 417篇 |
2000年 | 354篇 |
1999年 | 384篇 |
1998年 | 327篇 |
1997年 | 239篇 |
1996年 | 274篇 |
1995年 | 269篇 |
1994年 | 267篇 |
1993年 | 266篇 |
1992年 | 306篇 |
1991年 | 252篇 |
1990年 | 248篇 |
1989年 | 222篇 |
1988年 | 239篇 |
1987年 | 193篇 |
1986年 | 175篇 |
1985年 | 206篇 |
1984年 | 217篇 |
1983年 | 166篇 |
1982年 | 188篇 |
1981年 | 161篇 |
1980年 | 156篇 |
1979年 | 161篇 |
1978年 | 158篇 |
1977年 | 135篇 |
1976年 | 110篇 |
1975年 | 92篇 |
1973年 | 105篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
51.
52.
53.
54.
numb, a gene required in determination of cell fate during sensory organ formation in Drosophila embryos 总被引:8,自引:0,他引:8
Neurons and support cells of each sensory organ in Drosophila embryos are most likely derived from a single precursor cell. This cell lineage is affected in numb mutants. Morphological alterations of sensory structures, as well as changes in the number of cells expressing cell type-specific markers, indicate that sensory neurons in numb mutant embryos are transformed into lineage-related nonneuronal support cells. Thus the numb gene controls the fate of progeny derived from sensory organ precursors. The numb gene has been isolated by the plasmid rescue method. The structure of its predicted product is discussed. 相似文献
55.
V
H gene segments represent the products of the repeated duplication and subsequent diversification of a primordial V gene element. It is widely assumed that natural selection, operating via pathogens, has played the dominant role in this process. Here, we screen some 3.7 × 104 C
+
colonies of mitogen-activated B cells for the production of antibodies specific for phosphorylcholine or hen egg lysozyme and expression of the V
H X-24, S107, Q52, or J558 gene families. These gene families were expressed at frequencies proportional to their genomic complexity among both unselected and antigen-specific C
+
colonies. Thus, the capacity to encode equivalent antibody-combining sites is dispersed uniformly among V
Hfamilies. This result suggests that individual V
H genes have not evolved to address specific antigens. 相似文献
56.
Dynamic light-scattering studies on the effect of heat and disinfectants on spores of Bacillus cereus. 下载免费PDF全文
A D Molina-Garcia S E Harding L de Pieri N Jan W M Waites 《The Biochemical journal》1989,263(3):883-888
The relative stability of spores of Bacillus cereus grown at three different temperatures was examined by using quasi-elastic light scattering (q.l.s.) in conjunction with turbidity and scanning electron microscopy (s.e.m.). Cultures grown at 20, 30 and 40 degrees C (BC20, BC30 and BC40 respectively) were compared in terms of (i) their effective hydrodynamic radius, rH, as determined from q.l.s. and (ii) their gross morphology, as determined from s.e.m. The effects of autoclaving at 121.1 degrees C on both these properties was also examined. We observed (1) that cultures BC20 and BC30 appeared to have similar values for rH, whereas that of BC40 appeared some 50% higher, and (2) BC40 had a correspondingly much lower heat resistance (its structural integrity was lost after about 20 min autoclaving, whereas that of BC20 and BC30 was retained even after 80 min autoclaving). These data were in good agreement with independent measurements of heat-resistance coefficients. Changes in the hydrodynamic radius, polydispersity (both using q.l.s.) and turbidity were monitored with time on addition of the disinfectants sodium hypochlorite and peracetic acid; again BC40 appeared to have a lower resistance. 相似文献
57.
Karl H. Summer Dominik Klein Nada de Ruiter Josef Abel 《Biological trace element research》1989,21(1):165-169
In the present study we report on the effects of commonly used nonsteroidal antiinflammatory drugs on metallothionein (MT)
and MT-I mRNA levels. A single dose of chloroquine (100 mg/kg), diclofenac (100 mg/kg), indomethacin (10 mg/kg), or piroxicam
(100 mg/kg) was administered ip to C57B1 mice. After 18 h, MT levels were determined with a Cd-saturation radioassay. MT-I
mRNA levels were measured by Northern Blot analyses using a probe containing the mouse MT-I gene. All drugs tested caused
an increase in the MT content of the liver but not of the kidneys and lung. The lowest and highest effects were observed with
chloroquine (8 times the control value) and diclofenac (18 times), respectively. In accordance with the stimulation of MT
synthesis, increased accumulation of hepatic MT-I mRNA could be demonstrated.
These results indicate that elevated MT levels may contribute to the effectiveness of nonsteroidal antiinflammatory drugs
in the treatment of rheumatoid arthritis (RA). 相似文献
58.
Miroslav Pátek Jan Nešvera Jitka Hochmannová 《Applied microbiology and biotechnology》1989,31(1):65-69
Summary Four hybrid plasmids were constructed from the cryptic plasmid pAM330 (from Brevibacterium lactofermentum; 4.5 kb) and the broadhost-range plasmid pGV1106 (9.0 kb; Kmr Smr) isolated from Escherichia coli. All of them were mobilized from E. coli into the Gram-negative methylotrophic bacterium Methylobacillus sp. and two of these constructs (pCEM300 and pCEM400) were transferred by transformation into B. flavum and Corynebacterium glutamicum. Their kanamycin-resistance determinant coming from Gram-negative hosts was expressed in these Gram-positive bacteria. Both pCEM300 and pCEM400 are very stably maintained in B. flavum and represent suitable vectors for gene cloning in coryneform producers of amino acids. 相似文献
59.
A Klein M Kosz-Vnenchak Z Madeja A Szuster 《Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society》1989,27(1):11-17
Three RSV-transformed rat cell lines: GCA, W12 and XC were characterized as to their ability to anchorage-independent growth in comparison to normal rat kidney (NRK-49F) cells. Differences in the threshold density (TD) and colony forming efficiency (CFE) of the investigated cells are described. The ability of virally transformed cells to stimulation of soft agar colony formation of NRK cells in coculture assay was presented. The production of TGFs-like factors by GCA, W12 and XC cells was suggested. 相似文献
60.