全文获取类型
收费全文 | 108139篇 |
免费 | 9286篇 |
国内免费 | 58篇 |
专业分类
117483篇 |
出版年
2022年 | 760篇 |
2021年 | 1569篇 |
2020年 | 957篇 |
2019年 | 1175篇 |
2018年 | 1497篇 |
2017年 | 1391篇 |
2016年 | 2349篇 |
2015年 | 4057篇 |
2014年 | 4344篇 |
2013年 | 5757篇 |
2012年 | 7282篇 |
2011年 | 7321篇 |
2010年 | 4787篇 |
2009年 | 4244篇 |
2008年 | 6132篇 |
2007年 | 6266篇 |
2006年 | 6007篇 |
2005年 | 5907篇 |
2004年 | 5769篇 |
2003年 | 5426篇 |
2002年 | 5319篇 |
2001年 | 1240篇 |
2000年 | 958篇 |
1999年 | 1317篇 |
1998年 | 1560篇 |
1997年 | 1130篇 |
1996年 | 1058篇 |
1995年 | 993篇 |
1994年 | 970篇 |
1993年 | 1022篇 |
1992年 | 994篇 |
1991年 | 855篇 |
1990年 | 778篇 |
1989年 | 782篇 |
1988年 | 762篇 |
1987年 | 656篇 |
1986年 | 687篇 |
1985年 | 762篇 |
1984年 | 883篇 |
1983年 | 737篇 |
1982年 | 926篇 |
1981年 | 886篇 |
1980年 | 739篇 |
1979年 | 554篇 |
1978年 | 618篇 |
1977年 | 569篇 |
1976年 | 552篇 |
1975年 | 432篇 |
1974年 | 504篇 |
1973年 | 485篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
31.
32.
33.
W. Kelley Thomas J. T. Vida Linda M. Frisse Manuel Mundo James G. Baldwin 《Journal of nematology》1997,29(3):250-254
To effectively integrate DNA sequence analysis and classical nematode taxonomy, we must be able to obtain DNA sequences from formalin-fixed specimens. Microdissected sections of nematodes were removed from specimens fixed in formalin, using standard protocols and without destroying morphological features. The fixed sections provided sufficient template for multiple polymerase chain reaction-based DNA sequence analyses. 相似文献
34.
The potential for reducing the occurrence of shallow landslides through targeted reforestation of critical parts of a river basin is explored through mathematical modelling. Through the systematic investigation of land management options, modelling allows the optimum strategies to be selected ahead of any real intervention in the basin. Physically based models, for which the parameters can be evaluated using physical reasoning, offer particular advantages for predicting the effects of possible future changes in land use and climate. Typically a physically based landslide model consists of a coupled hydrological model (for soil moisture) and a geotechnical slope stability model, along with an impact model, such as basin sediment yield. An application of the SHETRAN model to the 65.8-km2 Guabalcón basin in central Ecuador demonstrates a technique for identifying the areas of a basin most susceptible to shallow landsliding and for quantifying the effects of different vegetation covers on landslide incidence. Thus, for the modelled scenario, increasing root cohesion from 300 to 1500 Pa causes a two-thirds reduction in the number of landslides. Useful information can be obtained even on the basis of imperfect data availability but model output should be interpreted carefully in the light of parameter uncertainty. 相似文献
35.
36.
James B Munro Roger B Altman Chang‐Shung Tung Kevin Y Sanbonmatsu Scott C Blanchard 《The EMBO journal》2010,29(4):770-781
A key intermediate in translocation is an ‘unlocked state’ of the pre‐translocation ribosome in which the P‐site tRNA adopts the P/E hybrid state, the L1 stalk domain closes and ribosomal subunits adopt a ratcheted configuration. Here, through two‐ and three‐colour smFRET imaging from multiple structural perspectives, EF‐G is shown to accelerate structural and kinetic pathways in the ribosome, leading to this transition. The EF‐G‐bound ribosome remains highly dynamic in nature, wherein, the unlocked state is transiently and reversibly formed. The P/E hybrid state is energetically favoured, but exchange with the classical P/P configuration persists; the L1 stalk adopts a fast dynamic mode characterized by rapid cycles of closure and opening. These data support a model in which P/E hybrid state formation, L1 stalk closure and subunit ratcheting are loosely coupled, independent processes that must converge to achieve the unlocked state. The highly dynamic nature of these motions, and their sensitivity to conformational and compositional changes in the ribosome, suggests that regulating the formation of this intermediate may present an effective avenue for translational control. 相似文献
37.
Charles L. Nunn Peter H. Thrall Kelly Stewart Alexander H. Harcourt 《Evolutionary ecology》2008,22(4):519-543
Emerging infectious diseases threaten a wide diversity of animals, and important questions remain concerning disease emergence
in socially structured populations. We developed a spatially explicit simulation model to investigate whether—and under what
conditions—disease-related mortality can impact rates of pathogen spread in populations of polygynous groups. Specifically,
we investigated whether pathogen-mediated dispersal (PMD) can occur when females disperse after the resident male dies from
disease, thus carrying infections to new groups. We also examined the effects of incubation period and virulence, host mortality
and rates of background dispersal, and we used the model to investigate the spread of the virus responsible for Ebola hemorrhagic
fever, which currently is devastating African ape populations. Output was analyzed using regression trees, which enable exploration
of hierarchical and non-linear relationships. Analyses revealed that the incidence of disease in single-male (polygynous)
groups was significantly greater for those groups containing an average of more than six females, while the total number of
infected hosts in the population was most sensitive to the number of females per group. Thus, as expected, PMD occurs in polygynous
groups and its effects increase as harem size (the number of females) increases. Simulation output further indicated that
population-level effects of Ebola are likely to differ among multi-male–multi-female chimpanzees and polygynous gorillas,
with larger overall numbers of chimpanzees infected, but more gorilla groups becoming infected due to increased dispersal
when the resident male dies. Collectively, our results highlight the importance of social system on the spread of disease
in wild mammals. 相似文献
38.
39.
Murine Cytomegalovirus m02 Gene Family Protects against Natural Killer Cell-Mediated Immune Surveillance 下载免费PDF全文
Sofia A. Oliveira Se-Ho Park Peter Lee Albert Bendelac Thomas E. Shenk 《Journal of virology》2002,76(2):885-894
The murine cytomegalovirus m02 gene family encodes putative type I membrane glycoproteins named m02 through m16. A subset of these genes were fused to an epitope tag and cloned into an expression vector. In transfected and murine cytomegalovirus-infected cells, m02, m04, m05, m06, m07, m09, m10, and m12 localized to cytoplasmic structures near the nucleus, whereas m08 and m13 localized to a filamentous structure surrounding the nucleus. Substitution mutants lacking the m02 gene (SMsubm02) or the entire m02 gene family (SMsubm02-16) grew like their wild-type parent in cultured cells. However, whereas SMsubm02 was as pathogenic as the wild-type virus, SMsubm02-16 was markedly less virulent. SMsubm02-16 produced less infectious virus in most organs compared to wild-type virus in BALB/c and C57BL/6J mice, but it replicated to wild-type levels in the organs of immunodeficient gamma(c)/Rag2 mice, lacking multiple cell types including natural killer cells, and in C57BL/6J mice depleted of natural killer cells. These results argue that one or more members of the m02 gene family antagonize natural killer cell-mediated immune surveillance. 相似文献
40.