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101.
James P. Ferris 《Origins of life and evolution of the biosphere》1987,17(3-4):219-219
102.
Stephen Chan James Orenberg Noam Lahav 《Origins of life and evolution of the biosphere》1987,17(2):121-134
The adsorption of 5-AMP and 5-CMP was studied in saturated solutions of several soluble mineral salts (NaCl, Na2SO4, MgCl2·6H2O, MgSO4·7H2O, CaCl2·2H2O, CaSO4·2H2O, SrCl2·6H2O, SrSO4, and ZnSO4·7H2O) as a function of pH, ionic strength, and surface area of the solid salt. The adsorption shows a pH dependence; this can be correlated with the charge on the nucleotide molecule which is determined by the state of protonation of the N-1 nitrogen of 5-AMP or N-3 nitrogen of 5-CMP and the phosphate oxygens. The adsorption which results from the binding between the nucleotide molecule and the salt surface is proposed as being due to electrostatic forces. It was concluded that the adsorption was reversible in nature. The adsorption shows a strong dependence upon ionic strength and decreases with increasing ionic strength. Surface area is shown to be an important factor in evaluating and comparing the magnitude of adsorption of nucleotides onto various mineral salts. The implications of the results of the study are discussed in terms of the importance of soluble mineral salts as adsorption sites in the characterization of the adsorption reactions of an adsorbed template in biogeochemical cycles. 相似文献
103.
Molecular Forms of Acetylcholinesterase and Butyrylcholinesterase in Human Plasma and Cerebrospinal Fluid 总被引:1,自引:0,他引:1
John R. Atack Elaine K. Perry James R. Bonham Robert H. Perry 《Journal of neurochemistry》1987,48(6):1845-1850
The measurement of cholinesterase activities in either plasma or cerebrospinal fluid (CSF) may ultimately prove to be relevant in the diagnosis of neurological and neuropsychiatric disorders. However, studies to date have examined only total enzyme activities. Therefore in the present study we have examined the distribution of the individual molecular forms of both acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) in plasma and CSF using sucrose density gradient centrifugation. Although the total activities of AChE were of the same order of magnitude in plasma and CSF, there was a considerable difference (120-500-fold) between total BChE activity in the CSF and the BChE-rich plasma. The analysis of the individual molecular forms revealed that the predominant molecular species of AChE and BChE in the CSF--both lumbar and ventricular--was the G4 form. The G4 form also constituted the majority of the plasma BChE activity and, on average, over half (56%) of the plasma AChE activity. The significance of the AChE and BChE molecular form compositions of both plasma and CSF and their possible relationship to pathological states are discussed. 相似文献
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A single locus, diallelic selection model with female and male viability differences is studied. If the variables are ratios of allele frequencies in each sex, a 2-dimensional difference equation describes the model. Because of the strong monotonicity of the resulting map, every initial genotypic structure converges to an equilibrium structure assuming that no equilibrium has eigenvalues on the unit circle.Partially supported by funds provided by a Science and Education Grant to the USDA-Forest Service, Southeastern Forest Experiment Station, Population Genetics of Forest Trees Research Unit, Raleigh, USASupported by a grant from the Max Kade Foundation, New York, USA 相似文献
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The ability of virulent and avirulent strains ofVibrio vulnificus to overcome iron limitations by using iron bound to iron-binding proteins was examined. While no strains were able to obtain iron from lactoferrin or ferritin when these proteins were not fully saturated with iron, growth was enhanced by the iron-saturated form of these proteins. None of the strains was able to scavange iron from 30% saturated transferrin, but there were strain differences in the ability to obtain iron from the saturated form. The virulent strains were able to compete more efficiently with transferrin when it was fully saturated with iron than were the avirulent strains. 相似文献
110.
The partition of native Escherichia coli -galactosidase and of two different fusion proteins comprised mainly of -galactosidase from E. coli was studied in aqueous two-phase systems composed of polyethylene glycol (PEG) and dextran. These fusions contain an amino-terminal segment from the E. coli outer membrane protein F (OmpF) and a linker peptide. Differences in the partition pattern could be observed for the three enzymes despite their similarity. Decreased polymer concentrations in the phase system increased the partition coefficient for all three -galactosidases. 相似文献