全文获取类型
收费全文 | 49901篇 |
免费 | 4550篇 |
国内免费 | 30篇 |
专业分类
54481篇 |
出版年
2022年 | 361篇 |
2021年 | 758篇 |
2020年 | 452篇 |
2019年 | 567篇 |
2018年 | 701篇 |
2017年 | 647篇 |
2016年 | 1039篇 |
2015年 | 1751篇 |
2014年 | 1934篇 |
2013年 | 2542篇 |
2012年 | 3217篇 |
2011年 | 3277篇 |
2010年 | 2126篇 |
2009年 | 1902篇 |
2008年 | 2705篇 |
2007年 | 2812篇 |
2006年 | 2742篇 |
2005年 | 2626篇 |
2004年 | 2526篇 |
2003年 | 2398篇 |
2002年 | 2297篇 |
2001年 | 538篇 |
2000年 | 444篇 |
1999年 | 607篇 |
1998年 | 729篇 |
1997年 | 547篇 |
1996年 | 469篇 |
1995年 | 427篇 |
1994年 | 411篇 |
1993年 | 440篇 |
1992年 | 475篇 |
1991年 | 411篇 |
1990年 | 402篇 |
1989年 | 389篇 |
1988年 | 364篇 |
1987年 | 326篇 |
1986年 | 350篇 |
1985年 | 375篇 |
1984年 | 453篇 |
1983年 | 372篇 |
1982年 | 477篇 |
1981年 | 491篇 |
1980年 | 389篇 |
1979年 | 320篇 |
1978年 | 334篇 |
1977年 | 293篇 |
1976年 | 328篇 |
1975年 | 220篇 |
1974年 | 275篇 |
1973年 | 271篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
221.
James E. Cloern Brian E. Cole Raymond L. J. Wong Andrea E. Alpine 《Hydrobiologia》1985,129(1):153-176
Detailed surveys throughout San Francisco Bay over an annual cycle (1980) show that seasonal variations of phytoplankton biomass, community composition, and productivity can differ markedly among estuarine habitat types. For example, in the river-dominated northern reach (Suisun Bay) phytoplankton seasonality is characterized by a prolonged summer bloom of netplanktonic diatoms that results from the accumulation of suspended particulates at the convergence of nontidal currents (i.e. where residence time is long). Here turbidity is persistently high such that phytoplankton growth and productivity are severely limited by light availability, the phytoplankton population turns over slowly, and biological processes appear to be less important mechanisms of temporal change than physical processes associated with freshwater inflow and turbulent mixing. The South Bay, in contrast, is a lagoon-type estuary less directly coupled to the influence of river discharge. Residence time is long (months) in this estuary, turbidity is lower and estimated rates of population growth are high (up to 1–2 doublings d–1), but the rapid production of phytoplankton biomass is presumably balanced by grazing losses to benthic herbivores. Exceptions occur for brief intervals (days to weeks) during spring when the water column stratifies so that algae retained in the surface layer are uncoupled from benthic grazing, and phytoplankton blooms develop. The degree of stratification varies over the neap-spring tidal cycle, so the South Bay represents an estuary where (1) biological processes (growth, grazing) and a physical process (vertical mixing) interact to cause temporal variability of phytoplankton biomass, and (2) temporal variability is highly dynamic because of the short-term variability of tides. Other mechanisms of temporal variability in estuarine phytoplankton include: zooplankton grazing, exchanges of microalgae between the sediment and water column, and horizontal dispersion which transports phytoplankton from regions of high productivity (shallows) to regions of low productivity (deep channels).Multi-year records of phytoplankton biomass show that large deviations from the typical annual cycles observed in 1980 can occur, and that interannual variability is driven by variability of annual precipitation and river discharge. Here, too, the nature of this variability differs among estuary types. Blooms occur only in the northern reach when river discharge falls within a narrow range, and the summer biomass increase was absent during years of extreme drought (1977) or years of exceptionally high discharge (1982). In South Bay, however, there is a direct relationship between phytoplankton biomass and river discharge. As discharge increases so does the buoyancy input required for density stratification, and wet years are characterized by persistent and intense spring blooms. 相似文献
222.
Properties and Partial Purification of Choline Acetyltransferase from the Nematode Caenorhabditis elegans 总被引:4,自引:4,他引:0
We have stabilized and studied choline acetyltransferase from the nematode Caenorhabditis elegans. The enzyme is soluble, and two discrete forms were resolved by gel filtration. The larger of these two forms (MW approximately 154,000) was somewhat unstable and in the presence of 0.5 M NaI was converted to a form indistinguishable from the "native" small form (MW approximately 71,000). We have purified the small form of the enzyme greater than 3,300-fold by a combination of gel filtration, ion-exchange chromatography, and nucleotide affinity chromatography. The purified preparation has a measured specific activity of 3.74 mumol/min/mg protein, and is free of acetylcholinesterase and acetyl-CoA hydrolase activities. The Vmax of the purified enzyme is stimulated by NaCl, with half-maximal stimulation at 80 mM NaCl. The Km for each substrate is also affected by salt, but in different manners from each other and the Vmax; the kinetic parameter Vmax/Km thus changes significantly as a function of the salt concentration. 相似文献
223.
224.
Summary
Bothriocephalus specimens from one Ctenopharyngodon idella (O+) were fixed using three different techniques and the scolex morphology was examined using scanning electron microscopy. The scolices were compared with figures of the five species of Bothriocephalus and one species of Schyzocotyle previously described from cyprinid fishes. The taxonomic history of the Bothriocephalus species parasitizing cyprinid fish is reviewed. It is concluded that there is only one Bothriocephalus species parasitizing cyprinid fishes, and that continued use should be made of the name B. acheilognathi Yamaguti, 1934. ac]19840326 相似文献
225.
James C. Lacey Jr. Leo M. Hall Dail W. Mullins Jr. 《Origins of life and evolution of the biosphere》1985,16(1):69-79
The genetic code appears to be a logic matrix in which, generally speaking, there is a correlation between the hydrophobicities of amino acids and their anticodonic nucleotides. There are several exceptions to this generality, however, and using previous data on hydrophobicity and binding constants, coupled with new data on reaction rates, we rationalize several of the anticodonic assignments. 相似文献
226.
Yeast communities in necroses of organpipe cactus (Stenocereus thurberi) were surveyed at 3 localities in Arizona. Quantitative analysis of random samples allows comparisons of the types and numbers of yeasts at 3 levels: within plants, between plants within a locality, and between localities. The analysis shows that the major source of variability is between plants. This pattern is identical with the pattern shown by agria cactus (Stenocereus gummosus) and is thought to be due to sampling different successional stages. No significant differences in estimates of the effective number of yeast species (ENS) in agria and organpipe samples were found. Comparisons of agria, organpipe, and prickly pear (Opuntia) cacti support the hypothesis that cactus chemistry is an important determinant of the yeast community structure which, in turn, is an important determinant of the diversity ofDrosophila species which utilize necrotic cacti as feeding and breeding substrates. 相似文献
227.
Synopsis Aspects of the life history of Barbus anoplus were studied in Lake le Roux, a turbid man-made lake on the Orange River, South Africa. This minnow underwent a population
explosion and successfully colonized the shoreline of the newly-formed lake during the early phases of reservoir filling.
Male and female B. anoplus reach sexual maturity in one year at about 40 mm fork length. They have a multiple spawning habit with the first spawning
in November–January and the second in February–March. The growth of the two resulting cohorts is discussed. It is proposed
that the offspring from the second spawning not only acts as a ‘back-up’ but is capable of prolonging the life of that year-class
into an additional reproductive season. Most of the minnows die after their second summer, but more offspring from the second
spawning, especially females, live into a third summer. Females attain a larger maximum size (73 mm FL) and age (3–4 years)
than males (60 mm FL, 2–3 years). B. anoplus is small and short-lived with a high seasonal reproductive potential, which is in contrast to the larger Barbus species in the Orange River system. These life-history traits enable the species to colonize and successfully inhabit unstable
environments and probably account for its widespread distribution. 相似文献
228.
Dr. Edward B. Blanchard James Jaccard Frank Andrasik Patricia Guarnieri Susan E. Jurish 《Applied psychophysiology and biofeedback》1985,10(1):63-68
Comparisons are made of self-reported medical costs from a sample of headache patients who underwent various combinations of relaxation training and biofeedback training. The average costs for the 2 years prior to self-regulatory treatment were $955±480 (3 SEM) for 45 patients; for the 2 years after completing treatment the average costs were $52±28 (3 SEM) for patients. Within the limitations of the study, medical costs do seem to have been markedly reduced.This research was supported by a grant from NINCDS, NS-15235. 相似文献
229.
Using a combination of a primary growth retardant, mefluidide, a synergistic additive, chlorsulfuron, a detergent to enhance penetration (X-77), and a herbicide, 2,4-D, to provide for control of broadleaf weeds, full-season management of bluegrass (Poa pratensis L.)—tall fescue (Festuca arundinaceae Schreb.) mixtures along roadsides has been achieved. A single spray application is made in the spring, and no additional herbicide applications or mechanical mowing are needed. The treatment is effective with greater than 90% control of fescue seed heads. Those few seed heads that do form remain short. It is economical. The costs of materials and application are equal to or less than the cost of a single mowing cycle. The treatment is environmentally safe when applied in early spring before most agricultural crops have been planted. The effectiveness and low cost of the combination derive from laboratory and greenhouse observations that various materials, herein referred to as additives, often only weakly effective as growth retardants themselves, will interact synergistically with mefluidide to provide overall treatment effectiveness at application rates that are economical. Using this principle, a combination suitable for roadside vegetation management was devised, field-tested for 2 years under actual use conditions, and found to be effective for full-season vegetation management of mixed bluegrass—tall fescue turf to permit considerable cost savings when compared to three-cycle mechanical mowing. 相似文献
230.
A method is described for the preparation of enriched populations of ciliated cells from rabbit tracheas. Following protease digestion of tracheal lumen tissue, cells were subjected to centrifugal elutriation. This produced two cell fractions of interest: an 8 µm diameter fraction believed to be composed largely of basal cells, and a 15 µm diameter fraction containing a mixture of ciliated cells and Clara cells. Further treatment of the 15 µm cells with a dextran/polyethylene glycol/phosphate buffer system resulted in separation of a highly enriched ciliated cell fraction (84.3 ± 2.7% ciliated cells with 6.5 ± 1.5% Clara cells) from a fraction containing both ciliated cells (42.0 ± 2.1%) and Clara cells (27.0 ± 3.5%). The yield of cells in the enriched ciliated cell fraction was 0.68 ± 0.09 × 106 cells/ trachea. Analysis of mixed-function oxidase activity in tracheal cells showed 7-ethoxycoumarin deethylase and coumarin hydroxylase activities to be present in the 8 µm cells as well as in ciliated cells and Clara cells. Enzyme activities measured in the ciliated cells (152 ± 66 pmol/ min/ mg protein or 51.2 ± 20.5 pmol/ min/ 106 cells for 7-ethoxycoumarin deethylase and 31.7 ± 15.4 pmol/ min/ mg protein or 10.5 ± 4.8 pmol/ min/ 106 cells for coumarin hydroxylase) were not attributable to contamination with Clara cells.Abbreviations CD
cell digest
- DNase
deoxyribonuclease I
- E-1
first elutriator fraction
- E-2
second elutriator fraction
- E-3
third elutriator fraction
- 7-Ec
7-ethoxycoumarin
- FCS
fetal calf serum
- HEPES
N-2-hydroxy-ethylpiperazine-N-2-ethanesulfonic acid
- HpBS
HEPES-buffered salt solution
- NADH
reduced nicotinamide adenine dinucleotide
- NADPH
reduced nicotinamide adenine dinucleotide phosphate
- NBT
nitro blue tetrazolium
- PEG
Carbowax polyethylene glycol 6000 相似文献