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81.
Hossnieh Kafshdar Jalali Abdolreza Salamatzadeh Arezou Kafshdar Jalali Hamed Haddad Kashani Salman Ahmadi Asbchin Khosro Issazadeh 《Probiotics and antimicrobial proteins》2016,8(1):41-45
The main drawback of current antibiotic therapies is the emergence and rapid increase in antibiotic resistance. Nocardiae are aerobic, Gram-positive, catalase-positive, non-motile actinomycetes. Nocardia brasiliensis was reported as antibiotic producer. The purpose of the study was to determine antibacterial activity of N. brasiliensis PTCC 1422 against isolated Enterobacteriaceae from urinary tract infections (UTIs). The common bacteria from UTIs were isolated from hospital samples. Antimicrobial susceptibility test was performed for the isolated pathogens using Kirby–Bauer disk diffusion method according to clinical and Laboratory Standards Institute guideline. Antagonistic activity of N. brasiliensis PTCC 1422 was examined with well diffusion methods. Supernatant of N. brasiliensis PTCC 1422 by submerged culture was analyzed with gas chromatography–mass spectrometry. Isolated strains included Escherichia coli, Klebsiella pneumoniae, Serratia marcescens and Proteus mirabilis. The most common pathogen isolated was E. coli (72.5 %). Bacterial isolates revealed the presence of high levels of antimicrobial resistances to ceftriaxone and low levels of resistance to cephalexin. Supernatant of N. brasiliensis PTCC 1422 showed antibacterial activity against all of the isolated microorganisms in well diffusion method. The antibiotic resistance among the uropathogens is an evolving process, so a routine surveillance to monitor the etiologic agents of UTI and the resistance pattern should be carried out timely to choose the most effective empirical treatment by the physicians. Our present investigation indicates that the substances present in the N. brasiliensis PTCC 1422 could be used to inhibit the growth of human pathogen. Antibacterial resistance among bacterial uropathogen is an evolving process. Therefore, in the field on the need of re-evaluation of empirical treatment of UTIs, our present. The study has demonstrated that N. brasiliensis PTCC 1422 has a high potential for the treatment of UTIs. 相似文献
82.
Elham Yezdani Jalal Jalali Sendi Arash Zibaee Mohammad Ghadamyari 《Comptes rendus biologies》2010,333(1):17-22
The pyralid moth, Glyphode pyloalis Walker, is an important pest of the mulberry. Amylases are the hydrolytic enzymes that catalyze the hydrolysis of the α-D-(1,4)-glucan linkage in glycogen and other related carbohydrates. Laboratory-reared fifth stadium larvae were randomly selected; the midgut (MG) and the salivary glands (SG) were removed by dissection under a dissecting microscope and α-amylase activity was assayed using the dinitrosalicylic acid procedure. The activity of α-amylase in the MG and the SG were 0.011 and 0.0018 μmol/min, respectively. The optimal pH and temperature for α-amylase were 9 for MG at 37–40 °C and 10 for SG at 37 °C respectively. Various concentrations of compounds (NaCl, KCl, MgCl2, Urea, EDTA, SDS and CaCl2) had differential effects on the enzyme activity. Plant amylase inhibitors may play an important role against insect pests. Hence, the characterization of digestive enzymes and the examination of their inhibitors may be a useful tool in future management of this important mulberry pest. 相似文献
83.
M Ameri Shah Reza SL Mousavi Gargari I Rasooli M Jalali Nadoushan W Ebrahimizadeh 《World journal of microbiology & biotechnology》2012,28(7):2513-2519
Helicobacter pylori is a Gram-negative spiral bacterium that colonizes human gastric mucosa causing infection. In this study aiming at inhibition of H. pylori infection we made an attempt to evaluate immunogenicity of the total (UreC) and C-terminal (UreCc) fragments of H. pylori urease. Total UreC and its C-terminal fragment were expressed in E. coli. Recombinant proteins were analyzed by SDS-PAGE and western blot and then purified by Ni-NTA affinity chromatography. Female C57BL6/j mice were immunized with the purified proteins (UreC and UreCc). Antibody titers from isolated sera were measured by ELISA. Immunized mice were then challenged by oral gavage with live H. pylori Sydney strain SS1. Total of 109 CFU were inoculated into stomach of immunized and unimmunized healthy mice three times each at one day interval. Eight weeks after the last inoculation, the blood sample was collected and the serum antibody titer was estimated by ELISA. Stomach tissues from control and experimental animal groups were studied histopathologically. UreC and UreCc yielded recombinant proteins of 61 and 31 kDa respectively. ELIZA confirmed establishment of immunity and the antibodies produced thereby efficiently recognized H. pylori and inhibited its colonization in vivo. Pathological analysis did not reveal established infection in immunized mice challenged with H. pylori. The results support the idea that UreC and UreCc specific antibodies contribute to protection against H. pylori infections. 相似文献
84.
Mohammad Taha Jalali Abdorrahim Absalan Alireza Mohseni Gholam Abbas Kaydani Zeinab Deris Zayeri 《生物学前沿》2017,12(6):442-447
Objectives
Thrombotic episodes occurred frequently in beta-thalassemia major (BTM) patients, leading to hypercoagulability of plasma. Protein Z (PZ) is a vitamin-K-dependent anti-coagulation factor that plays a role in the human homeostatic process. The objective of the current study is to investigate the distribution pattern of PZ plasma concentrations between BTM patients and the normal population in Ahvaz city, the center of Khuzestan province, southwest of Iran.Material and Methods
Forty confirmed BTM patients and 40 healthy volunteers were evaluated for complete blood count (CBC) indices and PZ plasma levels. CBC samples were measured using an automated cell counter, and PZ was assayed with an immunoassay method. Statistical analysis was conducted using SPSS software. The ROC curve and binary logistic regression estimated the sensitivity, specificity, and Odd’s ratio for PZ measurement.Results
The mean±SD of the PZ plasma level in normal individuals was 1.68±0.63 μg/mL, and in BTM patients, it was 1.10±0.52 μg/mL. This shows a significant reduction of PZ in BTM patients statistically (CI = 0.99; p<0.001). Further, the mean±SD of the PZ plasma levels in BTM patients who received washed red blood cells was not significantly different from that of patients undergoing packed red blood cell therapy (CI = 0.95; p = 0.320). The area under the curve (AUC) for PZ was 0.759 (p = 0.00). The cut-off value = 1.4 μg/mL of the PZ plasma level had at least 70% sensitivity and specificity in BTM patients.Discussion
Several epidemiologic studies have shown thromboembolism episodes in BTM patients. In the current study, PZ was reduced significantly in BTMs.Conclusion
We noticed that BTMs have lower plasma PZ concentration might be predisposed to BTM.85.
Animal fascioliasis in Khuzestan, southwestern Iran 总被引:1,自引:0,他引:1
86.
NBCe1 (SLC4A4) a potential pH regulator in enamel organ cells during enamel development in the mouse
87.
Knowledge of the rate of decrease of nutrients from soils resulting from poor water quality application is essential for long-term planning of crop production while minimizing the impact on groundwater quality. In this study, we examined the effect of Ca2+ concentration of irrigation water on phosphorus (P) leaching and kinetic release in columns of sandy soil. Phosphorous sorption in the presence of CaCl2 solutions with Ca2+ concentrations of 3, 5, 10, and 15 mM CaCl2 was determined to understand the transport and leaching of P in the sandy soil. The geochemical Visual MINTEQ was used to calculate saturation indices. A considerable number of leachate samples contained P at concentrations that could cause eutrophication. Total P leached from soil due to application of different CaCl2 solutions ranged from 1.7 to 1.8 kg ha?1 after 20 pore volumes had passed through the soil. Comparison of the leaching experiments results with the kinetic desorption data indicated that leaching removed on average 50 times less P than cumulative P desorbed by successive extractions with different CaCl2 solutions. Leaching in presence of different CaCl2 solutions was controlled by rate-limited dissolution of calcium hydroxyappatite and ß-tricalcium phosphate. 相似文献
88.
Rustas BO Nørgaard P Jalali AR Nadeau E 《Animal : an international journal of animal bioscience》2010,4(1):67-75
This study examined the effects of physical form and stage of maturity at harvest of whole-crop barley silage (WCBS) on feed intake, eating and rumination activity, diet selection and faecal particle size in dairy steers. Whole-crop barley was harvested and ensiled in round bales. Eight dairy steers (live weight (LW): 350 ± 10 kg) in a duplicated 4 × 4 Latin square design were fed WCBS harvested at heading or dough stage of maturity in long form or chopped in a 2 × 2 factorial treatment arrangement. The WCBS was supplemented with soybean meal. Daily dry matter (DM) intake increased by 7% (P < 0.05) due to chopping of dough-stage silage but was unaffected by chopping of heading-stage silage. The steers fed chopped, but not those fed long dough-stage silage, selected for starch in the WCBS (P < 0.001). The neutral detergent fibre (NDF) intake was 5% higher (P < 0.01) for heading than for dough-stage silage and was associated with lower concentration of indigestible NDF (96 v. 170 g/kg DM). Rate of intake of DM and NDF was 37% higher (P < 0.001), daily eating time was 24% shorter (P < 0.001) and daily chewing time was 8% shorter (P < 0.05) for chopped silage compared with long silage but there was no effect of maturity. Daily rumination time was not affected by treatments, whereas rumination and chewing times per kg NDF intake were 15% and 13% higher (P < 0.05), respectively, for dough-stage than for heading-stage silage. The proportion of faecal particles retained on a 1 mm screen was 30% higher (P < 0.001) due to chopping and 45% higher (P < 0.001) due to delayed harvest. Chopping the dough-stage silage reduced the proportion of grain in faeces from 97 to 43 g/kg DM (P < 0.05) indicating higher starch digestibility. In conclusion, chopping increased DM intake of WCBS when harvested at dough stage but not at heading stage of maturity. 相似文献
89.
90.
Knowledge of the rate of release of phosphorus (P) from soils resulting from poor water quality application is essential for long-term planning of crop production while minimizing the impact on groundwater quality. In this study, we examined the effect of sodium adsorption ratio (SAR) and Ca:Mg ratio of water on P release of some calcareous soils from western Iran. Nine different solutions at a total electrolyte concentration of 100 mmolc l?1 and three levels of SAR (5, 15, 45) each with Ca:Mg ratios of 1:3, 1:1, or 3:1, prepared using solutions of NaCl, CaCl2, and MgCl2, were used to extract P from the soils. The geochemical Visual MINTEQ was used to calculate saturation indices and P species at the initial and end of P release. Significantly different quantities of P were extracted by the solutions. The maximum (average of five soils) (233.6 mg kg?1) and the minimum (162.9 mg kg?1) P were extracted by an SAR 45 solution with a Ca:Mg ratio of 1:3 and SAR 15 solution with Ca:Mg ratio of 3:1, respectively. Elovich model adequately described P release. The release rate for SAR 15 with Ca:Mg of 3:1 and SAR 45 with Ca:Mg 1:3 ranges from 16.3 to 31.3 mg kg?1 h?1 and from 20.0 to 32.8 mg kg?1 h?1, respectively. In the initial stage of P release the solution samples in most soils were saturated with respect to hydroxyapatite, octacalcium phosphate, ß-tricalcium phosphate, and undersaturated with respect to dicalcium phosphate dihydrate, dicalcium phosphate, and mangnesium phosphates. At the end of P release, all solutions were saturated with respect to hydroxyapatite and under saturated with respect to other phosphate minerals. The results imply that P release from soils could be increased during use of saline and sodic irrigation water containing high Mg concentration and that P fertilization management may need modification. 相似文献