全文获取类型
收费全文 | 230篇 |
免费 | 16篇 |
出版年
2023年 | 4篇 |
2022年 | 3篇 |
2021年 | 10篇 |
2020年 | 7篇 |
2019年 | 11篇 |
2018年 | 19篇 |
2017年 | 4篇 |
2016年 | 7篇 |
2015年 | 18篇 |
2014年 | 14篇 |
2013年 | 12篇 |
2012年 | 18篇 |
2011年 | 12篇 |
2010年 | 10篇 |
2009年 | 11篇 |
2008年 | 10篇 |
2007年 | 11篇 |
2006年 | 14篇 |
2005年 | 7篇 |
2004年 | 5篇 |
2003年 | 5篇 |
2002年 | 7篇 |
2001年 | 6篇 |
2000年 | 2篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1994年 | 1篇 |
1990年 | 1篇 |
1982年 | 1篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1977年 | 2篇 |
1973年 | 1篇 |
1971年 | 1篇 |
1968年 | 2篇 |
1967年 | 2篇 |
1966年 | 1篇 |
1965年 | 1篇 |
排序方式: 共有246条查询结果,搜索用时 15 毫秒
141.
Mehran Aflakparast Mathisca C. M. de Gunst Wessel N. van Wieringen 《Biometrical journal. Biometrische Zeitschrift》2018,60(3):547-563
Cross‐sectional studies may shed light on the evolution of a disease like cancer through the comparison of patient traits among disease stages. This problem is especially challenging when a gene–gene interaction network needs to be reconstructed from omics data, and, in addition, the patients of each stage need not form a homogeneous group. Here, the problem is operationalized as the estimation of stage‐wise mixtures of Gaussian graphical models (GGMs) from high‐dimensional data. These mixtures are fitted by a (fused) ridge penalized EM algorithm. The fused ridge penalty shrinks GGMs of contiguous stages. The (fused) ridge penalty parameters are chosen through cross‐validation. The proposed estimation procedures are shown to be consistent and their performance in other respects is studied in simulation. The down‐stream exploitation of the fitted GGMs is outlined. In a data illustration the methodology is employed to identify gene–gene interaction network changes in the transition from normal to cancer prostate tissue. 相似文献
142.
Mehran Pashirzad Mojtaba Shafiee Amir Avan Mikhail Ryzhikov Hamid Fiuji Amirhossein Bahreyni Majid Khazaei Saman Soleimanpour Seyed Mahdi Hassanian 《Journal of cellular physiology》2019,234(9):14601-14611
Crocin is the major component of saffron, which is used in phytomedicine for the treatment of several diseases including diabetes, fatty liver, depression, menstruation disorders, and, of special interest in this review, inflammatory diseases. Promising selective anti-inflammatory properties of this pharmacological active component have been observed in several studies. Saffron has been shown to exert anti-inflammatory properties against several inflammatory diseases and can be used as a novel therapeutic agent for the treatment of inflammatory diseases either alone or in combination with other standard anti-inflammatory agents. This review summarizes the protective role of saffron and its pharmacologically active constituents in the pathogenesis of inflammatory diseases including digestive diseases, dermatitis, asthma, atherosclerosis, and neurodegenerative diseases for a better understanding and hence a better management of these diseases. 相似文献
143.
144.
Krenitsky VP Delgado M Nadolny L Sahasrabudhe K Ayala L Clareen SS Hilgraf R Albers R Kois A Hughes K Wright J Nowakowski J Sudbeck E Ghosh S Bahmanyar S Chamberlain P Muir J Cathers BE Giegel D Xu L Celeridad M Moghaddam M Khatsenko O Omholt P Katz J Pai S Fan R Tang Y Shirley MA Benish B Blease K Raymon H Bhagwat S Henderson I Cole AG Bennett B Satoh Y 《Bioorganic & medicinal chemistry letters》2012,22(3):1427-1432
In this Letter we describe the optimization of an aminopurine lead (1) with modest potency and poor overall kinase selectivity which led to the identification of a series of potent, selective JNK inhibitors. Improvement in kinase selectivity was enabled by introduction of an aliphatic side chain at the C-2 position. CC-359 (2) was selected as a potential clinical candidate for diseases manifested by ischemia reperfusion injury. 相似文献
145.
146.
147.
148.
149.
Fitting C Parlato M Adib-Conquy M Memain N Philippart F Misset B Monchi M Cavaillon JM Adrie C 《PloS one》2012,7(6):e38916
Fast and reliable assays to precisely define the nature of the infectious agents causing sepsis are eagerly anticipated. New molecular biology techniques are now available to define the presence of bacterial or fungal DNA within the bloodstream of sepsis patients. We have used a new technique (VYOO?) that allows the enrichment of microbial DNA before a multiplex polymerase chain reaction (PCR) for pathogen detection provided by SIRS-Lab (Jena, Germany). We analyzed 72 sepsis patients and 14 non-infectious systemic inflammatory response syndrome (SIRS) patients. Among the sepsis patients, 20 had a positive blood culture and 35 had a positive microbiology in other biological samples. Of these, 51.4% were positive using the VYOO? test. Among the sepsis patients with a negative microbiology and the non-infectious SIRS, 29.4% and 14.2% were positive with the VYOO? test, respectively. The concordance in bacterial identification between microbiology and the VYOO? test was 46.2%. This study demonstrates that these new technologies offer great hopes, but improvements are still needed. 相似文献
150.
This study sought to test whether targeted overexpression of osteoactivin (OA) in cells of osteoclastic lineage, using the tartrate-resistant acid phosphase (TRAP) exon 1B/C promoter to drive OA expression, would increase bone resorption and bone loss in vivo. OA transgenic osteoclasts showed ~2-fold increases in OA mRNA and proteins compared wild-type (WT) osteoclasts. However, the OA expression in transgenic osteoblasts was not different. At 4, 8, and 15.3 week-old, transgenic mice showed significant bone loss determined by pQCT and confirmed by μ-CT. In vitro, transgenic osteoclasts were twice as large, had twice as much TRAP activity, resorbed twice as much bone matrix, and expressed twice as much osteoclastic genes (MMP9, calciton receptor, and ADAM12), as WT osteoclasts. The siRNA-mediated suppression of OA expression in RAW264.7-derived osteoclasts reduced cell size and osteoclastic gene expression. Bone histomorphometry revealed that transgenic mice had more osteoclasts and osteoclast surface. Plasma c-telopeptide (a resorption biomarker) measurements confirmed an increase in bone resorption in transgenic mice in vivo. In contrast, histomorphometric bone formation parameters and plasma levels of bone formation biomarkers (osteocalcin and pro-collagen type I N-terminal peptide) were not different between transgenic mice and WT littermates, indicating the lack of bone formation effects. In conclusion, this study provides compelling in vivo evidence that osteoclast-derived OA is a novel stimulator of osteoclast activity and bone resorption. 相似文献