Properties of Thermally Dewetted Thin Au Films on ITO-Coated Glass for Biosensing Applications

Plasmonics - Noble metal nanostructures are object of great interest due to their unique optical and electronic properties exploited in nanotechnology, medicine, biochemistry, and surface-enhanced...     

Embryo ecology: Developmental synchrony and asynchrony in the embryonic development of wild annual fish populations

Embryo–environment interactions are of paramount importance during the development of all organisms, and impacts during this period can echo far into later stages of ontogeny. African annual fish of the genus Nothobranchius live in temporary pools and their eggs survive the dry season in the dry bottom substrate of the pools by entering a facultative developmental arrest termed diapause. Uniquely among animals, the embryos (encased in eggs) may enter diapause at three different developmental stages. Such a system allows for the potential to employ different regulation mechanisms for each diapause. We sampled multiple Nothobranchius embryo banks across the progressing season, species, and populations. We present important baseline field data and examine the role of environmental regulation in the embryonic development of this unique system. We describe the course of embryo development in the wild and find it to be very different from the typical development under laboratory conditions. Development across the embryo banks was synchronized within and across the sampled populations with all embryos entering diapause I during the rainy season and diapause II during the dry season. Asynchrony occurred at transient phases of the habitat, during the process of habitat desiccation, and at the end of the dry season. Our findings reveal the significance of environmental conditions in the serial character of the annual fish diapauses.     

Protocol of single cells preparation for time of flight secondary ion mass spectrometry

There are several techniques like time of flight secondary ion mass spectrometry (ToF SIMS) that require a special protocol for preparation of biological samples, in particular, those containing single cells due to high vacuum conditions that must be kept during the experiment. Frequently, preparation methodology involves liquid nitrogen freezing what is not always convenient. In our studies, we propose and validate a protocol for preparation of single cells. It consists of four steps: (i) paraformaldehyde fixation, (ii) salt removal, (iii) dehydrating, and (iv) sample drying under ambient conditions. The protocol was applied to samples with single melanoma cells i.e. WM115 and WM266-4 characterized by similar morphology. The surface and internal structures of cells were monitored using atomic force, scanning electron and fluorescent microscopes, used to follow any potential protocol-induced alterations. To validate the proposed methodology for sample preparation, ToF SIMS experiments were carried out using C₆₀⁺ cluster ion beam. The applied principal component analysis (PCA) revealed that chemical changes on cell surface of melanoma cells were large enough to differentiate between primary and secondary tumor sites.     

The Use and Economic Value of Manna grass (Glyceria) in Poland from the Middle Ages to the Twentieth Century

Manna grass (mainly but not exclusively G. fluitans) used to be widely gathered in most lowland areas of the present territory of Poland and western and southern Belarus. It had an important function as a component of tribute paid to local landowners by villagers, which led to the persistence of manna gathering even when this was not sustainable for peasants themselves. Manna grass was always an expensive food due to its time consuming gathering, but appreciated for its sweet taste and often served as dessert. In the nineteenth century marshes shrank significantly and the payment of tribute disappeared from the local economy, which gradually led to the total abandonment of Glyceria use around 1914. This article provides a detailed overview of Glyceria use as food within the borders of the former Polish-Lithuanian Kingdom (now Poland, Lithuania, western Belarus and western Ukraine) based on archaeobotanical, historical and ethnographic sources. The evidence for the continued use of manna since at least medieval times is abundant in historical accounts and ethnographic studies, but little has been reported in archaeobotanical findings due to the relatively small amounts of Glyceria consumed.     

Unexpected cryptic species diversity of parasites of the family Xenidae (Strepsiptera) with a constant diversification rate over time

Parasitism is one of the most successful and ancient strategies. Due to the specialized lifestyle of parasites, they are usually affected by reductions and changes in their body plan in comparison with nonparasitic sister groups. Extreme environmental conditions may impose restraints on behavioural or physiological adaptations to a specific host and limit morphological changes associated with speciation. Such morphological homogeneity has led to the diversity of parasites being underestimated in morphological studies. By contrast, the species concept has dramatically changed in many parasitic groups during recent decades of study using DNA sequence data. Here we tested the phenomenon of cryptic species diversity in the twisted‐wing parasite family Xenidae (Strepsiptera) using nuclear and mitochondrial DNA sequence data for a broad sample of Xenidae. We used three quantitative methods of species delimitation from the molecular phylogenetic data – one distance‐based (ABGD) and two tree‐based (GMYC, bPTP). We found 77–96 putative species in our data and suggested the number of Xenidae species to be more diverse than expected. We identified 67 hosts to species level and almost half of them were not previously known as hosts of Xenidae. The mean number of host species per putative species varied between 1.39 and 1.55. The constant rate in net diversification can be explained by the flexibility of this parasitic group, represented by their ability to colonize new host lineages combined with passive long‐range dispersal by hosts.     

How the Geomagnetic Field Influences Life on Earth – An Integrated Approach to Geomagnetobiology

Origins of Life and Evolution of Biospheres - Earth is one of the inner planets of the Solar System, but – unlike the others – it has an oxidising atmosphere, relatively stable...