全文获取类型
收费全文 | 186篇 |
免费 | 16篇 |
出版年
2023年 | 1篇 |
2022年 | 2篇 |
2021年 | 5篇 |
2020年 | 8篇 |
2019年 | 5篇 |
2018年 | 3篇 |
2017年 | 3篇 |
2016年 | 6篇 |
2015年 | 10篇 |
2014年 | 15篇 |
2013年 | 18篇 |
2012年 | 20篇 |
2011年 | 15篇 |
2010年 | 8篇 |
2009年 | 6篇 |
2008年 | 7篇 |
2007年 | 15篇 |
2006年 | 6篇 |
2005年 | 5篇 |
2004年 | 6篇 |
2003年 | 11篇 |
2002年 | 6篇 |
2001年 | 1篇 |
2000年 | 2篇 |
1999年 | 3篇 |
1997年 | 2篇 |
1995年 | 3篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1982年 | 1篇 |
1981年 | 3篇 |
1979年 | 1篇 |
1973年 | 1篇 |
排序方式: 共有202条查询结果,搜索用时 31 毫秒
121.
Cecilia M Parrula Jagannatha Mysore Holly Burr Wendy Freebern Natasha Neef 《Comparative medicine》2015,65(3):271-276
A 4-y-old female cynomolgus macaque presented for veterinary evaluation prior to placement in a preclinical study showed markedly low platelet counts that continued to decrease over time. Physical examination over the next several days showed areas of pale red discoloration in forelimbs, anterior thorax, and inguinal area and multifocal pinpoint areas of erythema or scabs. An area of dark red discoloration approximately 2 cm in diameter on the dorsal surface of the tongue was discovered on day 9. The macaque was euthanized, and histopathologic evaluation showed multifocal, ulcerative or erosive, hemorrhagic, lymphohistiocytic and neutrophilic glossitis and tonsillitis. The lesions on the tongue were associated with opportunistic fungi consistent with Candida albicans. The bone marrow showed megakaryocytic hyperplasia. There was no evidence of increased consumption of platelets, sequestration of platelets, or bone marrow suppression. The monkey was serologically negative for simian retrovirus, SIV, and simian T-lymphotropic virus. In light of cases reported in humans, immune-mediated destruction of platelets due to autoantibodies secondary to Candida albicans infection was considered. However, we were unable to detect antiplatelet antibodies on the platelet surface or in serum to support this etiology; therefore idiopathic thrombocytopenia was diagnosed. To our knowledge, this case represents the second reported observation of acquired thrombocytopenia in a nonhuman primate and the first reported observation in a cynomolgus macaque.Abbreviations: SVV, simian varicella virusDrug-induced thrombocytopenia is a serious but relatively common clinical condition, because numerous drugs have been associated with development of DIT. The diagnosis of drug-induced thrombocytopenia is usually made after the exclusion of all other possible causes, such as infectious agents and artifactual decreases due to clumping of platelets in vitro (pseudothrombocytopenia), and the establishment of a temporal relationship between the administration of a drug and the development of thrombocytopenia. Consequently, scientists involved with preclinical studies in NHP need to be aware of nondrug-related cases of unexplained marked thrombocytopenia like the one we describe in this case report because they may confound a drug-related effect. 相似文献
122.
Xiao Wang Juanjuan Zhang Maofeng Chai Lu Han Xiaohua Cao Jing Zhang Yiming Kong Chunxiang Fu Zeng-Yu Wang Kirankumar S.Mysore Jiangqi Wen Chuanen Zhou 《植物学报(英文版)》2023,65(10):2279-2291
Compound leaf development requires the coordination of genetic factors, hormones, and other signals. In this study, we explored the functions of Class Ⅱ KNOTTED-like homeobox(KNOXII)genes in the model leguminous plant Medicago truncatula. Phenotypic and genetic analyses suggest that Mt KNOX4, 5 are able to repress leafet formation, while Mt KNOX3, 9, 10 are not involved in this developmental process. Further investigations have shown that Mt KNOX4 represses the CK signal transduction, which is d... 相似文献
123.
Identification of T-DNA tagged Arabidopsis mutants that are resistant to transformation by Agrobacterium 总被引:2,自引:0,他引:2
J. Nam K. S. Mysore C. Zheng M. K. Knue A. G. Matthysse S. B. Gelvin 《Molecular genetics and genomics : MGG》1999,261(3):429-438
We have identified T-DNA tagged Arabidopsis mutants that are resistant to transformation by Agrobacterium tumefaciens (rat mutants). These mutants are highly recalcitrant to the induction of both crown gall tumors and phosphinothricin-resistant calli. The results of transient GUS (β-glucuronidase) assays suggest that some of these mutants are blocked at an early step in the Agrobacterium-mediated transformation process, whereas others are blocked at a step subsequent to translocation of T-DNA into the nucleus. Attachment of Agrobacterium to roots of the mutants rat1 and rat3 was decreased under various incubation conditions. In most mutants, the transformation-deficient phenotype co-segregated with the kanamycin resistance encoded by the mutagenizing T-DNA. In crosses with susceptible wild-type plants, the resistance phenotype of many of these mutants segregated either as a semi-dominant or dominant trait. 相似文献
124.
125.
126.
Sudarshana MR Plesha MA Uratsu SL Falk BW Dandekar AM Huang TK McDonald KA 《Plant biotechnology journal》2006,4(5):551-559
A novel cucumber mosaic virus inducible viral amplicon (CMViva) expression system has been developed that allows for tightly regulated chemically inducible expression of heterologous genes in plant hosts. Transient production of recombinant α1 -antitrypsin (rAAT), a human blood protein, was demonstrated in Nicotiana benthamiana leaves. The highest production levels were obtained by co-infiltrating leaves with Agrobacterium tumefaciens cells containing CMViva carrying the AAT gene and A. tumefaciens cells carrying a binary vector constitutively expressing the gene silencing suppressor p19. Accumulation of up to thirty-fold more rAAT was observed in leaves (24 mg per 100 g leaf tissue) when compared with the expression levels observed using the cauliflower mosaic virus (CaMV) 35S promoter. Significantly, 70% of the rAAT produced using the CMViva expression system was found to be biologically active, a 170-fold increase in functional protein compared with the CaMV 35S expression system. 相似文献
127.
Mysore V. Tejesvi Basavanna Mahesh Monnanda S. Nalini Harishchandra S. Prakash Kukkundoor R. Kini Ven Subbiah Hunthrike S. Shetty 《World journal of microbiology & biotechnology》2005,21(8-9):1535-1540
Summary Fungal endophytes reside in healthy tissues of all terrestrial plant taxa studied to date and are diverse and abundant in
tropical woody angiosperms. Endophytic fungi were isolated from Terminalia arjuna, an important ethno pharmacological plant extensively used in ayurvedic medicines to treat heart ailments. Isolations were
made from symptomless fresh inner bark as well as twig samples of five plants collected from three locations of riparian vegetation
during two seasons (monsoon and winter) of 2003 and 2004. Two hundred and seventy eight isolates, representing 22 genera,
were obtained from both seasons. Monsoon seasonal isolations representing 22 genera showed greater diversity. Coelomycetes
were more numerous during the winter season than hyphomycetes and ascomycetes. Among the endophytes, the genus Pestalotiopsis dominated the endophyte assemblage of T. arjuna collected from different locations, dominance was greater during the winter season than the monsoon season. Endophytic colonization
frequency was greater in inner bark (18.5%) than twigs (4.6%). The genera Pestalotiopsis (54.5%), Chaetomium (10.5%) and Myrothecium (9%) were the most predominant endophytes. Rarefaction indices indicated the highest expected number of species for bark
samples, monsoon isolations and location 1 (Mysore). 相似文献
128.
129.
Salicylic acid and systemic acquired resistance play a role in attenuating crown gall disease caused by Agrobacterium tumefaciens 总被引:4,自引:0,他引:4
下载免费PDF全文
![点击此处可从《Plant physiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
We investigated the effects of salicylic acid (SA) and systemic acquired resistance (SAR) on crown gall disease caused by Agrobacterium tumefaciens. Nicotiana benthamiana plants treated with SA showed decreased susceptibility to Agrobacterium infection. Exogenous application of SA to Agrobacterium cultures decreased its growth, virulence, and attachment to plant cells. Using Agrobacterium whole-genome microarrays, we characterized the direct effects of SA on bacterial gene expression and showed that SA inhibits induction of virulence (vir) genes and the repABC operon, and differentially regulates the expression of many other sets of genes. Using virus-induced gene silencing, we further demonstrate that plant genes involved in SA biosynthesis and signaling are important determinants for Agrobacterium infectivity on plants. Silencing of ICS (isochorismate synthase), NPR1 (nonexpresser of pathogenesis-related gene 1), and SABP2 (SA-binding protein 2) in N. benthamiana enhanced Agrobacterium infection. Moreover, plants treated with benzo-(1,2,3)-thiadiazole-7-carbothioic acid, a potent inducer of SAR, showed reduced disease symptoms. Our data suggest that SA and SAR both play a major role in retarding Agrobacterium infectivity. 相似文献
130.
Mysore Gopal Tejeswini Hebbale Vasanth Sowmya Shimoga Prabhakar Swarnalatha 《Archives Of Phytopathology And Plant Protection》2013,46(5):564-570
Natural additives are in demand for the control of microbial growth in foods. Several natural compounds including essential oils (EOs) are being explored for food uses. In the present investigation, the antifungal activity of cinnamaldehyde, eugenol, peppermint and clove EOs and their combinations was evaluated against 12 species of Aspergillus, Fusarium, Penicillium and Rhizopus in in vitro and tomato fruit system (in-vivo). The EOs were able to inhibit complete growth of tested fungi at or below 0.6% level and 80?μL of EOs (except peppermint oil) in in vitro condition and tomato system, respectively. The fractional inhibitory studies showed either additive or indifferent effect by combining eugenol and peppermint, and indifferent or antagonist effect by combining the cinnamaldehyde and clove in both in vitro and in vivo studies. The findings may be useful for application of these EOs in foods, but their effects on organoleptic quality of foods need to be investigated. 相似文献