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101.
DNA variation at the invertase locus invGE/GF is associated with tuber quality traits in populations of potato breeding clones 总被引:5,自引:0,他引:5 下载免费PDF全文
Li L Strahwald J Hofferbert HR Lübeck J Tacke E Junghans H Wunder J Gebhardt C 《Genetics》2005,170(2):813-821
Starch and sugar content of potato tubers are quantitative traits, which are models for the candidate gene approach for identifying the molecular basis of quantitative trait loci (QTL) in noninbred plants. Starch and sugar content are also important for the quality of processed products such as potato chips and French fries. A high content of the reducing sugars glucose and fructose results in inferior chip quality. Tuber starch content affects nutritional quality. Functional and genetic models suggest that genes encoding invertases control, among other things, tuber sugar content. The invGE/GF locus on potato chromosome IX consists of duplicated invertase genes invGE and invGF and colocalizes with cold-sweetening QTL Sug9. DNA variation at invGE/GF was analyzed in 188 tetraploid potato cultivars, which have been assessed for chip quality and tuber starch content. Two closely correlated invertase alleles, invGE-f and invGF-d, were associated with better chip quality in three breeding populations. Allele invGF-b was associated with lower tuber starch content. The potato invertase gene invGE is orthologous to the tomato invertase gene Lin5, which is causal for the fruit-sugar-yield QTL Brix9-2-5, suggesting that natural variation of sugar yield in tomato fruits and sugar content of potato tubers is controlled by functional variants of orthologous invertase genes. 相似文献
102.
Fox BM Natero R Richard K Connors R Roveto PM Beckmann H Haller K Golde J Xiao SH Kayser F 《Bioorganic & medicinal chemistry letters》2011,21(8):2460-2467
We discovered novel pyrrolidine MCHR1 antagonist 1 possessing moderate potency. Profiling of pyrrolidine 1 demonstrated that it was an inhibitor of the hERG channel. Investigation of the structure-activity relationship of this class of pyrrolidines allowed us to optimize the MCHR1 potency and decrease the hERG inhibition. Increasing the acidity of the amide proton by converting the benzamide in lead 1 to an anilide provided single digit nanomolar MCHR1 antagonists while replacing the dimethoxyphenyl ring of 1 with alkyl groups possessing increased polarity dramatically reduced the hERG inhibition. 相似文献
103.
A detailed facies study of Early Permian strata within NE Svalbard reveals a fundamental change of the depositional setting, from a restricted-marine, warm-water carbonate platform to an open-marine, temperate-water, mixed siliciclastic-carbonate ramp. The uppermost strata of the Gipshuken Formation (Templet and Sørfonna members; Sakmarian–early Artinskian?) consist of microbialites (algal mats), mudstones, bioclastic/peloidal limestones, carbonate breccias and Microcodium facies reflecting peritidal platform areas and supratidal sabkhas. A mixed heterozoan/reduced photozoan assemblage indicates temperate-water conditions within neighboring deeper, open-marine mid-platform areas, while warm-water conditions still prevailed within inner platform zones. In contrast, the lowermost strata of the overlying Kapp Starostin Formation (Vøringen Member; late Artinskian?–Kungurian) show a fully heterozoan biotic assemblage reflecting temperate water conditions within open-marine, storm-dominated, nearshore to transitional offshore areas of a mixed carbonate-siliciclastic ramp. The Vøringen Member comprises three facies associations, which form a shallowing-upward sequence subsequent to an initial transgression. The sediments reflect bryozoan bioherms in most distal areas, followed by stacked tempestites of sandy brachiopodal shell banks and Skolithos piperocks, grading into broad sand flats in most proximal areas of the inner ramp. The above environmental change is regarded as a regional event taken place across the entire shelf along the northern margin of Pangea and is attributed to paleoclimatic, paleoceanographic, as well as paleogeographic changes, possibly related to the overall northwards drift of the supercontinent. An abrupt increase in terrigenous input coinciding with this change is ascribed to the uplift of a new local source area, probably to the north or east of the investigation area. 相似文献
104.
Katharina Danhauser Sven?W. Sauer Tobias?B. Haack Thomas Wieland Christian Staufner Elisabeth Graf Johannes Zschocke Tim?M. Strom Thorsten Traub Jürgen?G. Okun Thomas Meitinger Georg?F. Hoffmann Holger Prokisch Stefan K?lker 《American journal of human genetics》2012,91(6):1082-1087
Abnormalities in metabolite profiles are valuable indicators of underlying pathologic conditions at the molecular level. However, their interpretation relies on detailed knowledge of the pathways, enzymes, and genes involved. Identification and characterization of their physiological function are therefore crucial for our understanding of human disease: they can provide guidance for therapeutic intervention and help us to identify suitable biomarkers for monitoring associated disorders. We studied two individuals with 2-aminoadipic and 2-oxoadipic aciduria, a metabolic condition that is still unresolved at the molecular level. This disorder has been associated with varying neurological symptoms. Exome sequencing of a single affected individual revealed compound heterozygosity for an initiating methionine mutation (c.1A>G) and a missense mutation (c.2185G>A [p.Gly729Arg]) in DHTKD1. This gene codes for dehydrogenase E1 and transketolase domain-containing protein 1, which is part of a 2-oxoglutarate-dehydrogenase-complex-like protein. Sequence analysis of a second individual identified the same missense mutation together with a nonsense mutation (c.1228C>T [p.Arg410∗]) in DHTKD1. Increased levels of 2-oxoadipate in individual-derived fibroblasts normalized upon lentiviral expression of the wild-type DHTKD1 mRNA. Moreover, investigation of L-lysine metabolism showed an accumulation of deuterium-labeled 2-oxoadipate only in noncomplemented cells, demonstrating that DHTKD1 codes for the enzyme mediating the last unresolved step in the L-lysine-degradation pathway. All together, our results establish mutations in DHTKD1 as a cause of human 2-aminoadipic and 2-oxoadipic aciduria via impaired turnover of decarboxylation 2-oxoadipate to glutaryl-CoA. 相似文献
105.
Wen-Xing Ding Fengli Guo Hong-Min Ni Abigail Bockus Sharon Manley Donna B. Stolz Eeva-Liisa Eskelinen Hartmut Jaeschke Xiao-Ming Yin 《The Journal of biological chemistry》2012,287(50):42379-42388
Mitochondrial homeostasis via mitochondrial dynamics and quality control is crucial to normal cellular functions. Mitophagy (mitochondria removed by autophagy) stimulated by a mitochondrial uncoupler, carbonyl cyanide m-chlorophenylhydrazone (CCCP), requires Parkin, but it is not clear why Parkin is crucial to this process. We found that in the absence of Parkin, carbonyl cyanide m-chlorophenylhydrazone induced the formation of mitochondrial spheroids. Mitochondrial spheroid formation is also induced in vivo in the liver by acetaminophen overdose, a condition causing severe oxidative mitochondrial damages and liver injury. Mitochondrial spheroids could undergo a maturation process by interactions with acidic compartments. The formation of this new structure required reactive oxygen species and mitofusins. Parkin suppressed these mitochondrial dynamics by promoting mitofusin degradation. Consistently, genetic deletion of mitofusins without concomitant expression of Parkin was sufficient to prevent mitochondrial spheroid formation and resumed mitophagy. Mitochondrial spheroid formation and mitophagy could represent different strategies of mitochondrial homeostatic response to oxidative stress and are reciprocally regulated by mitofusins and Parkin. 相似文献
106.
107.
Ahmed M. Abouzid Thomas Frischmuth Holger Jeske 《Molecular & general genetics : MGG》1988,212(2):252-258
Summary The putative replicative form of the abutilon mosaic virus (AbMV), a geminivirus, was purified from infected plants. It was shown to consist of a bipartite genome of 2660 and 2640 bp. This double-stranded DNA has a closed or relaxed circular conformation and part of it is packed in nucleoprotein complexes with a chromatin-like structure. Similarities between the geminiviruses and the animal simian virus 40 are discussed against this back-ground.Cloning was performed under L2/B1 conditions according to the licence of the ZKBS 1526/1This article is based on a doctoral study by A.A. and T.F. in the Faculty of Biology, University of Hamburg 相似文献
108.
Exogenous Isolation of Antibiotic Resistance Plasmids from Piggery Manure Slurries Reveals a High Prevalence and Diversity of IncQ-Like Plasmids 总被引:6,自引:2,他引:6 下载免费PDF全文
Kornelia Smalla Holger Heuer Antje Gtz Dagmar Niemeyer Ellen Krgerrecklenfort Erhard Tietze 《Applied microbiology》2000,66(11):4854-4862
Antibiotic resistance plasmids were exogenously isolated in biparental matings with piggery manure bacteria as plasmid donors in Escherichia coli CV601 and Pseudomonas putida UWC1 recipients. Surprisingly, IncQ-like plasmids were detected by dot blot hybridization with an IncQ oriV probe in several P. putida UWC1 transconjugants. The capture of IncQ-like plasmids in biparental matings indicates not only their high prevalence in manure slurries but also the presence of efficiently mobilizing plasmids. In order to elucidate unusual hybridization data (weak or no hybridization with IncQ repB or IncQ oriT probes) four IncQ-like plasmids (pIE1107, pIE1115, pIE1120, and pIE1130), each representing a different EcoRV restriction pattern, were selected for a more thorough plasmid characterization after transfer into E. coli K-12 strain DH5α by transformation. The characterization of the IncQ-like plasmids revealed an astonishingly high diversity with regard to phenotypic and genotypic properties. Four different multiple antibiotic resistance patterns were found to be conferred by the IncQ-like plasmids. The plasmids could be mobilized by the RP4 derivative pTH10 into Acinetobacter sp., Ralstonia eutropha, Agrobacterium tumefaciens, and P. putida, but they showed diverse patterns of stability under nonselective growth conditions in different host backgrounds. Incompatibility testing and PCR analysis clearly revealed at least two different types of IncQ-like plasmids. PCR amplification of total DNA extracted directly from different manure samples and other environments indicated the prevalence of both types of IncQ plasmids in manure, sewage, and farm soil. These findings suggest that IncQ plasmids play an important role in disseminating antibiotic resistance genes. 相似文献
109.
Karsten Schnatbaum Victor Solis‐Mezarino Daniil Pokrovsky Frederike Schfer Dennis Nagl Lars Hornberger Johannes Zerweck Tobias Knaute Julia Avramova‐Nehmer Mike Schutkowski Veit Hornung Holger Wenschuh Moritz Carl Vlker‐Albert Axel Imhof Ulf Reimer 《Proteomics》2020,20(10)
Targeted proteomics depends on the availability of stable isotope labeled (SIL) peptide standards, which for absolute protein quantification need to be absolutely quantified. In the present study, three new approaches for absolute quantification of SIL peptides are developed. All approaches rely on a quantification tag (Qtag) with a specific UV absorption. The Qtag is attached to the peptide during synthesis and is removed by tryptic digestion under standard proteomics workflow conditions. While one quantification method (method A) is designed to allow the fast and economic production of absolutely quantified SIL peptides, two other methods (methods B and C) are developed to enable the straightforward re‐quantification of SIL peptides after reconstitution to control and monitor known problems related to peptide solubility, precipitation, and adhesion to vials. All methods yield consistent results when compared to each other and when compared to quantification by amino acid analysis. The precise quantitation methods are used to characterize the in vivo specificity of the H3 specific histone methyltransferase EZH2. 相似文献
110.
Kerstin Sander Yvonne von Coburg Jean-Claude Camelin Xavier Ligneau Oliver Rau Manfred Schubert-Zsilavecz Jean-Charles Schwartz Holger Stark 《Bioorganic & medicinal chemistry letters》2010,20(5):1581-1584
Antagonists of the human histamine H3 receptor (hH3R) often contain a second basic moiety, which is well known to boost affinity on this histamine receptor subtype. Here, we prepared compounds with acidic moieties of different pKa values to figure out that the hH3R tolerates these functionalities when added to a common pharmacophore blueprint. Depending on the acidic, electronic and steric features the designed ligands showed hH3R affinities in the nanomolar concentration range. Additionally, selected ligands were tested but failed as dual acting hH3R/hPPAR (human peroxisome proliferator-activated receptor) ligands. 相似文献