全文获取类型
收费全文 | 590篇 |
免费 | 33篇 |
出版年
2024年 | 1篇 |
2023年 | 1篇 |
2022年 | 7篇 |
2021年 | 17篇 |
2020年 | 14篇 |
2019年 | 10篇 |
2018年 | 12篇 |
2017年 | 12篇 |
2016年 | 17篇 |
2015年 | 27篇 |
2014年 | 44篇 |
2013年 | 33篇 |
2012年 | 54篇 |
2011年 | 53篇 |
2010年 | 28篇 |
2009年 | 29篇 |
2008年 | 43篇 |
2007年 | 35篇 |
2006年 | 24篇 |
2005年 | 18篇 |
2004年 | 21篇 |
2003年 | 21篇 |
2002年 | 15篇 |
2001年 | 17篇 |
2000年 | 17篇 |
1999年 | 14篇 |
1998年 | 3篇 |
1997年 | 3篇 |
1996年 | 3篇 |
1995年 | 2篇 |
1994年 | 3篇 |
1993年 | 1篇 |
1992年 | 3篇 |
1991年 | 1篇 |
1989年 | 1篇 |
1987年 | 1篇 |
1973年 | 4篇 |
1972年 | 7篇 |
1971年 | 4篇 |
1970年 | 2篇 |
1967年 | 1篇 |
排序方式: 共有623条查询结果,搜索用时 15 毫秒
71.
72.
73.
74.
Polycomb group (PcG) proteins are key chromatin regulators implicated in multiple processes including embryonic development, tissue homeostasis, genomic imprinting, X-chromosome inactivation, and germ cell differentiation. The PcG proteins recognize target genomic loci through cis DNA sequences known as Polycomb Response Elements (PREs), which are well characterized in Drosophila. However, mammalian PREs have been elusive until two groups reported putative mammalian PREs recently. Consistent with the existence of mammalian PREs, here we report the identification and characterization of a potential PRE from human T cells. The putative human PRE has enriched binding of PcG proteins, and such binding is dependent on a key PcG component SUZ12. We demonstrate that the putative human PRE carries both genetic and molecular features of Drosophila PRE in transgenic flies, implying that not only the trans PcG proteins but also certain features of the cis PREs are conserved between mammals and Drosophila. 相似文献
75.
76.
Phylogenetic Characterization of Two Novel Commensal Bacteria Involved with Innate Immune Homeostasis in Drosophila melanogaster 下载免费PDF全文
Seong Woon Roh Young-Do Nam Ho-Won Chang Kyoung-Ho Kim Min-Soo Kim Ji-Hwan Ryu Sung-Hee Kim Won-Jae Lee Jin-Woo Bae 《Applied microbiology》2008,74(20):6171-6177
During a previous study on the molecular interaction between commensal bacteria and host gut immunity, two novel bacterial strains, A911T and G707T, were isolated from the gut of Drosophila melanogaster. In this study, these strains were characterized in a polyphasic taxonomic study using phenotypic, genetic, and chemotaxonomic analyses. We show that the strains represent novel species in the family Acetobacteraceae. Strain G707T, a highly pathogenic organism, represents a new species in the genus Gluconobacter, “Gluconobacter morbifer” sp. nov. (type strain G707 = KCTC 22116T = JCM 15512T). Strain A911T, dominantly present in the normal Drosphila gut community, represents a novel genus and species, designated “Commensalibacter intestini” gen. nov., sp. nov. (type strain A911 = KCTC 22117T = JCM 15511T). 相似文献
77.
Yeon Ho Je Jin Hee Chang Mi Hyang Kim Jong Yul Roh Byung Rae Jin David R. O''''Reilly 《Biotechnology letters》2001,23(21):1809-1817
A system is described for the rapid generation of Bombyx mori nucleopolyhedrovirus (BmNPV)-based expression vectors. A series of novel BmNPV genomes, that include a mini-F replicon and therefore can be maintained in Escherichia coli, have been generated. These genomes lack a portion of the essential ORF1629 gene and cannot replicate independently in insect cells. However, they can be used as parental genomes for the generation of expression vectors by cotransfection with a transfer plasmid that includes an intact ORF1629. Only recombinant viruses that have acquired the ORF1629 gene from the transfer vector, and have therefore also acquired the foreign gene of interest, can replicate after cotransfection. Parental genomes with and without a polyhedrin gene are described, enabling the generation of occlusion-positive and occlusion-negative recombinant viruses. Occlusion-positive expression vectors enable the oral infection of B. mori larvae and can therefore be used for the mass production of a foreign protein in infected insects. 相似文献
78.
79.
80.