全文获取类型
收费全文 | 1228篇 |
免费 | 146篇 |
专业分类
1374篇 |
出版年
2022年 | 17篇 |
2021年 | 27篇 |
2020年 | 11篇 |
2019年 | 15篇 |
2018年 | 23篇 |
2017年 | 15篇 |
2016年 | 43篇 |
2015年 | 46篇 |
2014年 | 53篇 |
2013年 | 57篇 |
2012年 | 66篇 |
2011年 | 52篇 |
2010年 | 29篇 |
2009年 | 33篇 |
2008年 | 31篇 |
2007年 | 29篇 |
2006年 | 33篇 |
2005年 | 28篇 |
2004年 | 38篇 |
2003年 | 38篇 |
2002年 | 20篇 |
2001年 | 23篇 |
2000年 | 28篇 |
1999年 | 23篇 |
1998年 | 13篇 |
1997年 | 10篇 |
1994年 | 11篇 |
1993年 | 12篇 |
1992年 | 27篇 |
1991年 | 29篇 |
1990年 | 24篇 |
1989年 | 19篇 |
1988年 | 36篇 |
1987年 | 31篇 |
1986年 | 20篇 |
1985年 | 30篇 |
1984年 | 34篇 |
1983年 | 17篇 |
1982年 | 12篇 |
1981年 | 14篇 |
1980年 | 27篇 |
1979年 | 26篇 |
1978年 | 27篇 |
1975年 | 21篇 |
1974年 | 9篇 |
1973年 | 16篇 |
1972年 | 9篇 |
1971年 | 11篇 |
1970年 | 10篇 |
1969年 | 13篇 |
排序方式: 共有1374条查询结果,搜索用时 15 毫秒
51.
52.
53.
Pajon A Ionides J Diprose J Fillon J Fogh R Ashton AW Berman H Boucher W Cygler M Deleury E Esnouf R Janin J Kim R Krimm I Lawson CL Oeuillet E Poupon A Raymond S Stevens T van Tilbeurgh H Westbrook J Wood P Ulrich E Vranken W Xueli L Laue E Stuart DI Henrick K 《Proteins》2005,58(2):278-284
Data management has emerged as one of the central issues in the high-throughput processes of taking a protein target sequence through to a protein sample. To simplify this task, and following extensive consultation with the international structural genomics community, we describe here a model of the data related to protein production. The model is suitable for both large and small facilities for use in tracking samples, experiments, and results through the many procedures involved. The model is described in Unified Modeling Language (UML). In addition, we present relational database schemas derived from the UML. These relational schemas are already in use in a number of data management projects. 相似文献
54.
So-Jung Kwon Kyuyong Han Suhyun Jung Jong-Eun Lee Seongsoon Park Yong-Pil Cheon Jade Hyunjung Lim 《BMC biotechnology》2009,9(1):73
Background
MPG is a cell-permeable peptide with proven efficiency to deliver macromolecular cargoes into cells. In this work, we examined the efficacy of MPG as an N-terminal tag in a fusion protein to deliver a protein cargo and its mechanism of transduction. 相似文献55.
56.
Deutsch EW Ball CA Berman JJ Bova GS Brazma A Bumgarner RE Campbell D Causton HC Christiansen JH Daian F Dauga D Davidson DR Gimenez G Goo YA Grimmond S Henrich T Herrmann BG Johnson MH Korb M Mills JC Oudes AJ Parkinson HE Pascal LE Pollet N Quackenbush J Ramialison M Ringwald M Salgado D Sansone SA Sherlock G Stoeckert CJ Swedlow J Taylor RC Walashek L Warford A Wilkinson DG Zhou Y Zon LI Liu AY True LD 《Nature biotechnology》2008,26(3):305-312
One purpose of the biomedical literature is to report results in sufficient detail that the methods of data collection and analysis can be independently replicated and verified. Here we present reporting guidelines for gene expression localization experiments: the minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE). MISFISHIE is modeled after the Minimum Information About a Microarray Experiment (MIAME) specification for microarray experiments. Both guidelines define what information should be reported without dictating a format for encoding that information. MISFISHIE describes six types of information to be provided for each experiment: experimental design, biomaterials and treatments, reporters, staining, imaging data and image characterizations. This specification has benefited the consortium within which it was developed and is expected to benefit the wider research community. We welcome feedback from the scientific community to help improve our proposal. 相似文献
57.
58.
Ornob Alam Rafal M Gutaker Cheng-Chieh Wu Karen A Hicks Kyle Bocinsky Cristina Cobo Castillo Stephen Acabado Dorian Fuller Jade A dAlpoim Guedes Yue-Ie Hsing Michael D Purugganan 《Molecular biology and evolution》2021,38(11):4832
The dispersal of rice (Oryza sativa) following domestication influenced massive social and cultural changes across South, East, and Southeast (SE) Asia. The history of dispersal across islands of SE Asia, and the role of Taiwan and the Austronesian expansion in this process remain largely unresolved. Here, we reconstructed the routes of dispersal of O. sativa ssp. japonica rice to Taiwan and the northern Philippines using whole-genome resequencing of indigenous rice landraces coupled with archaeological and paleoclimate data. Our results indicate that japonica rice found in the northern Philippines diverged from Indonesian landraces as early as 3,500 years before present (BP). In contrast, rice cultivated by the indigenous peoples of the Taiwanese mountains has complex origins. It comprises two distinct populations, each best explained as a result of admixture between temperate japonica that presumably came from northeast Asia, and tropical japonica from the northern Philippines and mainland SE Asia, respectively. We find that the temperate japonica component of these indigenous Taiwan populations diverged from northeast Asia subpopulations at about 2,600 BP, whereas gene flow from the northern Philippines had begun before ∼1,300 BP. This coincides with a period of intensified trade established across the South China Sea. Finally, we find evidence for positive selection acting on distinct genomic regions in different rice subpopulations, indicating local adaptation associated with the spread of japonica rice. 相似文献
59.
Wang CI Guncar G Forwood JK Teh T Catanzariti AM Lawrence GJ Loughlin FE Mackay JP Schirra HJ Anderson PA Ellis JG Dodds PN Kobe B 《The Plant cell》2007,19(9):2898-2912
The gene-for-gene mechanism of plant disease resistance involves direct or indirect recognition of pathogen avirulence (Avr) proteins by plant resistance (R) proteins. Flax rust (Melampsora lini) AvrL567 avirulence proteins and the corresponding flax (Linum usitatissimum) L5, L6, and L7 resistance proteins interact directly. We determined the three-dimensional structures of two members of the AvrL567 family, AvrL567-A and AvrL567-D, at 1.4- and 2.3-A resolution, respectively. The structures of both proteins are very similar and reveal a beta-sandwich fold with no close known structural homologs. The polymorphic residues in the AvrL567 family map to the surface of the protein, and polymorphisms in residues associated with recognition differences for the R proteins lead to significant changes in surface chemical properties. Analysis of single amino acid substitutions in AvrL567 proteins confirm the role of individual residues in conferring differences in recognition and suggest that the specificity results from the cumulative effects of multiple amino acid contacts. The structures also provide insights into possible pathogen-associated functions of AvrL567 proteins, with nucleic acid binding activity demonstrated in vitro. Our studies provide some of the first structural information on avirulence proteins that bind directly to the corresponding resistance proteins, allowing an examination of the molecular basis of the interaction with the resistance proteins as a step toward designing new resistance specificities. 相似文献
60.
A mutation in Tac1p, a transcription factor regulating CDR1 and CDR2, is coupled with loss of heterozygosity at chromosome 5 to mediate antifungal resistance in Candida albicans 下载免费PDF全文
Coste A Turner V Ischer F Morschhäuser J Forche A Selmecki A Berman J Bille J Sanglard D 《Genetics》2006,172(4):2139-2156