全文获取类型
收费全文 | 6551篇 |
免费 | 444篇 |
国内免费 | 3篇 |
专业分类
6998篇 |
出版年
2022年 | 35篇 |
2021年 | 68篇 |
2020年 | 34篇 |
2019年 | 59篇 |
2018年 | 62篇 |
2017年 | 53篇 |
2016年 | 96篇 |
2015年 | 192篇 |
2014年 | 224篇 |
2013年 | 303篇 |
2012年 | 338篇 |
2011年 | 363篇 |
2010年 | 293篇 |
2009年 | 250篇 |
2008年 | 338篇 |
2007年 | 367篇 |
2006年 | 362篇 |
2005年 | 338篇 |
2004年 | 357篇 |
2003年 | 348篇 |
2002年 | 356篇 |
2001年 | 92篇 |
2000年 | 77篇 |
1999年 | 104篇 |
1998年 | 127篇 |
1997年 | 84篇 |
1996年 | 83篇 |
1995年 | 96篇 |
1994年 | 76篇 |
1993年 | 89篇 |
1992年 | 72篇 |
1991年 | 63篇 |
1990年 | 74篇 |
1989年 | 57篇 |
1988年 | 55篇 |
1987年 | 39篇 |
1986年 | 52篇 |
1985年 | 36篇 |
1984年 | 63篇 |
1983年 | 57篇 |
1982年 | 69篇 |
1981年 | 76篇 |
1980年 | 65篇 |
1979年 | 55篇 |
1978年 | 47篇 |
1977年 | 42篇 |
1976年 | 34篇 |
1975年 | 35篇 |
1974年 | 36篇 |
1973年 | 18篇 |
排序方式: 共有6998条查询结果,搜索用时 15 毫秒
161.
模拟打制是史前考古研究中的重要实验考古方法之一,对于正确认识考古标本的类型和技术、解读当时人类的认知水平和知识积累具有不可取代的作用。本文简要回溯了模拟打制实验的发展史,并评述其应用现状。模拟打制实验在欧美有较长的发展与应用历史,但最终趋向了不同的发展方向。传统的模拟打制实验研究在欧洲得到了更加深入的继承和发展,而美国在上世纪90年代后期逐渐发展起了以定量控制、数理统计为核心的实验方法。本文以近期在山西丁村开展的角页岩模拟打制实验为例,介绍传统的模拟打制实验的基本流程和内容,包括实验的内容设计、实施及记录和分析等。本次实验结果肯定了硬锤锤击技术在丁村角页岩石核剥片和修理中的广泛应用,并在一定程度上否定了以往对使用碰砧法的推测。文章最后对模拟打制实验存在的问题和应用前景作了讨论和展望。 相似文献
162.
163.
Christine De Paillerets Jacques Gallay Michel Vincent Monique Rogard Annette Alfsen 《生物化学与生物物理学报:生物膜》1981,644(1):134-142
The lipid dynamics of the adrenocortical microsomal membranes was studied by monitoring the fluorescence anisotropy and excited state lifetime of a set of anthroyloxy fatty acid probes (2-, 7-, 9- and 12-(9-anthroyloxy)-stearic acid (AP) and 16-(9-anthroyloxy)palmitic acid (AS). It was found that a decreasing polarity gradient from the aqueous membrane interface to the membrane interior, was present. This gradient was not modified by the proteins, as evidenced by comparison of complete membranes and derived liposomes, suggesting that the anthroyloxy probes were not in close contact with the proteins. An important change of the value of the mean rotational relaxation time as a function of the position of the anthroyl ring along the acyl chain was evidenced. In the complete membranes, a relatively more fluid medium was evidenced in the C16 as compared to the C2 region, while the rotational motion appeared to be the most hindered at the C7–C9 level. In the derived liposomes, a similar trend was observed but the mobility was higher at all levels. The decrease of the mean rotational relaxation time was more important for 12-AS and 16-AP. Temperature dependence of the mean rotational relaxation time of 2-AS, 12-AS and 16-AP in the complete membranes revealed the existence of a lipid reorganization occurring around 27°C and concerning mainly the C16 region. The extent to which the acyl chain reacted to this perturbation at the C12 level depended on pH. The presence of proteins increased the apparent magnitude of this reorganization and also modified the critical temperature from approx. 23°C in the derived liposomes to approx. 27°C in the complete membranes. Thermal dependence of the maximum velocity of the , the second enzyme in the enzymatic sequence, responsible for the biosynthesis of the in the adrenal cortex microsomes, was studied. The activation energy of the catalyzed reaction was found to be low and constant () in the temperature range 16–40°C at pH 7.5, 8.5 and 9, corresponding to the minimum, intermediate and maximum rate, respectively. A drastic increase of the activation energy () was observed at temperature below 16°C at pH 7.5. A correlated change of the as function of temperature was detected; at 36°C while at 13°C the value shifted to 8.7. The pH range of the group ionization was narrower at 13°C. In contrast with the behaviour of the , the was apparently unaffected by the lipid reorganization at 27°C. It is suggested that this enzyme possesses a different and more fluid lipid environment than the bulk lipids. 相似文献
164.
Lanfermeijer FC Dijkhuis J Sturre MJ de Haan P Hille J 《Plant molecular biology》2003,52(5):1039-1051
In tomato, infections by tomato mosaic virus are controlled by durable Tm-22 resistance. In order to gain insight into the processes underlying disease resistance and its durability, we cloned and analysed the Tm-22 resistance gene and the susceptible allele, tm-2. The Tm-22 gene was isolated by transposon tagging using a screen in which plants with a destroyed Tm-22 gene survive. The Tm-22 locus consists of a single gene that encodes an 861 amino acid polypeptide, which belongs to the CC-NBS-LRR class of resistance proteins. The putative tm-2 allele was cloned from susceptible tomato lines via PCR with primers based on the Tm-22 sequence. Interestingly, the tm-2 gene has an open reading frame that is comparable to the Tm-22 allele. Between the tm-2 and the Tm-22 polypeptide 38 amino acid differences are present of which 26 are located in the second half of the LRR-domain. Susceptible tomato plants, which were transformed with the Tm-22 gene, displayed resistance against ToMV infection. In addition, virus specificity, displayed by the Tm-22 resistance was conserved in these transgenic lines. To explain the durability of this resistance, it is proposed that the Tm-22-encoded resistance is aimed at the Achilles' heel of the virus. 相似文献
165.
Dendritic cells (DCs) produce cytokines and are susceptible to cytokine-mediated activation. Thus, interaction of resting immature DCs with TLR ligands, for example nucleic acids, or with microbes leads to a cascade of pro-inflammatory cytokines and skewing of T cell responses. Conversely, several cytokines are able to trigger DC activation (maturation) via autocrine, for example TNF and plasmacytoid DCs, and paracrine, for example type I IFN and myeloid DCs, pathways. By controlling DC activation, cytokines regulate immune homeostasis and the balance between tolerance and immunity. The increased production and/or bioavailability of cytokines and associated alterations in DC homeostasis have been implicated in various human inflammatory and autoimmune diseases. Targeting these cytokines with biological agents as already is the case with TNF and IL-1 represents a success of immunology and the coming years will expand the range of cytokines as therapeutic targets in autoinflammatory and autoimmune pathology. 相似文献
166.
C Condé X Rambout M Lebrun A Lecat E Di Valentin F Dequiedt J Piette G Gloire S Legrand 《PloS one》2012,7(7):e41005
SHIP-1 is an inositol phosphatase predominantly expressed in hematopoietic cells. Over the ten past years, SHIP-1 has been described as an important regulator of immune functions. Here, we characterize a new inhibitory function for SHIP-1 in NOD2 signaling. NOD2 is a crucial cytoplasmic bacterial sensor that activates proinflammatory and antimicrobial responses upon bacterial invasion. We observed that SHIP-1 decreases NOD2-induced NF-κB activation in macrophages. This negative regulation relies on its interaction with XIAP. Indeed, we observed that XIAP is an essential mediator of the NOD2 signaling pathway that enables proper NF-κB activation in macrophages. Upon NOD2 activation, SHIP-1 C-terminal proline rich domain (PRD) interacts with XIAP, thereby disturbing the interaction between XIAP and RIP2 in order to decrease NF-κB signaling. 相似文献
167.
168.
169.
Jacques Friborg Jr. Wing-Pui Kong C. Clay Flowers Scarlett L. Flowers Yongnian Sun Kimberly E. Foreman Brian J. Nickoloff Gary J. Nabel 《Journal of virology》1998,72(12):10073-10082
The DNA sequence for Kaposi’s sarcoma-associated herpesvirus was originally detected in Kaposi’s sarcoma biopsy specimens. Since its discovery, it has been possible to detect virus in cell lines established from AIDS-associated body cavity-based B-cell lymphoma and to propagate virus from primary Kaposi’s sarcoma lesions in a human renal embryonic cell line, 293. In this study, we analyzed the infectivity of Kaposi’s sarcoma-associated herpesvirus produced from these two sources. Viral isolates from cultured cutaneous primary KS cells was transmitted to an Epstein-Barr virus-negative Burkitt’s B-lymphoma cell line, Louckes, and compared to virus induced from a body cavity-based B-cell lymphoma cell line. While propagation of body cavity-based B-cell lymphoma-derived virus was not observed in 293 cell cultures, infection with viral isolates obtained from primary Kaposi’s sarcoma lesions induced injury in 293 cells typical of herpesvirus infection and was associated with apoptotic cell death. Interestingly, transient overexpression of the Kaposi’s sarcoma-associated herpesvirus v-Bcl-2 homolog delayed the process of apoptosis and prolonged the survival of infected 293 cells. In contrast, the broad-spectrum caspase inhibitors Z-VAD-fmk and Z-DEVD-fmk failed to protect infected cell cultures, suggesting that Kaposi’s sarcoma-associated herpesvirus-induced apoptosis occurs through a Bcl-2-dependent pathway. Kaposi’s sarcoma-associated herpesvirus isolates from primary Kaposi’s sarcoma lesions and body cavity-based lymphomas therefore may differ and are likely to have distinct contributions to the pathophysiology of Kaposi’s sarcoma. 相似文献
170.