全文获取类型
收费全文 | 356篇 |
免费 | 27篇 |
出版年
2023年 | 4篇 |
2022年 | 5篇 |
2021年 | 12篇 |
2020年 | 3篇 |
2019年 | 6篇 |
2018年 | 5篇 |
2017年 | 1篇 |
2016年 | 14篇 |
2015年 | 20篇 |
2014年 | 18篇 |
2013年 | 23篇 |
2012年 | 32篇 |
2011年 | 21篇 |
2010年 | 11篇 |
2009年 | 12篇 |
2008年 | 22篇 |
2007年 | 23篇 |
2006年 | 28篇 |
2005年 | 18篇 |
2004年 | 11篇 |
2003年 | 16篇 |
2002年 | 11篇 |
2001年 | 3篇 |
1999年 | 5篇 |
1998年 | 5篇 |
1997年 | 4篇 |
1996年 | 4篇 |
1995年 | 2篇 |
1994年 | 5篇 |
1993年 | 2篇 |
1992年 | 4篇 |
1991年 | 5篇 |
1990年 | 3篇 |
1989年 | 3篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1986年 | 2篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1980年 | 4篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1977年 | 2篇 |
1976年 | 1篇 |
1975年 | 1篇 |
1973年 | 1篇 |
排序方式: 共有383条查询结果,搜索用时 68 毫秒
11.
Amaia Artal-Martinez de Narvajas Timothy S. Gomez Jin-San Zhang Alexander O. Mann Yoshiyuki Taoda Jacquelyn A. Gorman Marta Herreros-Villanueva Thomas M. Gress Volker Ellenrieder Luis Bujanda Do-Hyung Kim Alan P. Kozikowski Alexander Koenig Daniel D. Billadeau 《Molecular and cellular biology》2013,33(20):3983-3993
12.
O-linked glycosylation is a ubiquitous protein modification in organisms belonging to several kingdoms. Both microbial and host protein glycans are used by many pathogens for host invasion and immune evasion, yet little is known about the roles of O-glycans in viral pathogenesis. Reportedly, there is no single function attributed to O-glycans for the significant paramyxovirus family. The paramyxovirus family includes many important pathogens, such as measles, mumps, parainfluenza, metapneumo- and the deadly Henipaviruses Nipah (NiV) and Hendra (HeV) viruses. Paramyxoviral cell entry requires the coordinated actions of two viral membrane glycoproteins: the attachment (HN/H/G) and fusion (F) glycoproteins. O-glycan sites in HeV G were recently identified, facilitating use of the attachment protein of this deadly paramyxovirus as a model to study O-glycan functions. We mutated the identified HeV G O-glycosylation sites and found mutants with altered cell-cell fusion, G conformation, G/F association, viral entry in a pseudotyped viral system, and, quite unexpectedly, pseudotyped viral F protein incorporation and processing phenotypes. These are all important functions of viral glycoproteins. These phenotypes were broadly conserved for equivalent NiV mutants. Thus our results identify multiple novel and pathologically important functions of paramyxoviral O-glycans, paving the way to study O-glycan functions in other paramyxoviruses and enveloped viruses. 相似文献
13.
14.
Lu Fang Piyushkumar A. Mundra Fenling Fan Abby Galvin Jacquelyn M. Weir Gerard Wong Jaye Chin-Dusting Flavia Cicuttini Peter Meikle Anthony Michael Dart 《Metabolomics : Official journal of the Metabolomic Society》2016,12(8):136
Introduction
Rheumatoid arthritis (RA) is linked to increased cardiovascular morbidity and mortality, not completely explained by traditional risk factors. Importantly, the increased risk occurs despite lower levels of total and low-density lipoprotein cholesterol. Whilst systemic inflammation may be a factor, it is possible that changes in individual lipid species contribute to the increased cardiovascular risk.Objectives
In the present study, we characterized plasma lipidomic profiles in patients with RA in comparison with healthy controls.Methods
Patients with RA (n = 32) and age- and gender-matched healthy volunteers (n = 84) were recruited. Fasting plasma lipid profiles were measured using electrospray-ionisation tandem mass spectrometry. 24 lipid classes and subclasses were measured.Results
Patients with RA had normal total, low-density lipoprotein and high-density lipoprotein cholesterol, but higher triglycerides than controls. Five lipid classes (dihydroceramides, alkylphosphatidylethanolamine, alkenylphosphatidylethanolamine, lysophosphatidylinositol, phosphatidylserine) differed between patients with RA and controls. Then we measured 36 lipid species within these 5 classes and found that 11 lipid species were different between patients with RA and controls. Three lipid classes (dihydroceramides, lysophosphatidylinositol, phosphatidylserine) and 10 lipid species remained significantly associated with RA after adjusting for age, sex, body mass index, current smoking, systolic blood pressure and anti-hypertensive treatment in a binary logistic regression model.Conclusion
This study has identified lipid alterations in RA. These alterations of lipids warrant further investigation as they may be associated with accelerated atherosclerosis and joint inflammation in patient with RA.15.
Xu J Lee G Wang H Vierling JM Maher JJ 《American journal of physiology. Gastrointestinal and liver physiology》2004,287(3):G734-G741
Alpha-naphthylisothiocyanate (ANIT) is a hepatotoxin that causes severe neutrophilic inflammation around portal tracts and bile ducts. The chemotactic signals that provoke this inflammatory response are unknown. In this study, we addressed the possibility that ANIT upregulates CXC chemokines in the liver and that these compounds mediate hepatic inflammation and tissue injury after ANIT treatment. Mice treated with a single dose of ANIT (50 mg/kg) exhibited rapid hepatic induction of the CXC chemokine macrophage inflammatory protein-2 (MIP-2). MIP-2 derived primarily from hepatocytes, with no apparent contribution by biliary cells. In ANIT-treated mice, the induction of MIP-2 coincided with an influx of neutrophils to portal zones; this hepatic neutrophil recruitment was suppressed by 50% in mice that lack the receptor for MIP-2 (CXCR2(-/-)). Interestingly, despite their markedly reduced degree of hepatic inflammation, CXCR2(-/-) mice displayed just as much hepatocellular injury and cholestasis after ANIT treatment as wild-type mice. Moreover, after long-term exposure, ANIT CXCR2(-/-) mice developed liver fibrosis that was indistinguishable from that in wild-type mice. In summary, our data show that CXC chemokines are responsible for some of the hepatic inflammation that occurs in response to ANIT but that these compounds are not essential to the pathogenesis of either acute or chronic ANIT hepatotoxicity. 相似文献
16.
17.
18.
Jacquelyn A. Kallunki 《Brittonia》1998,50(4):500-513
Ticorea comprises five species, which occur in the Guianas, throughout the Amazonian basin, and on the lower eastern slopes of the Andes in Ecuador, Peru, and Bolivia. Two of the five are described here as new: T. diandra, from eastern Ecuador and adjacent Peru, and T. froesii, from Maranhão and Pará, Brazil. 相似文献
19.
Irene Mittermann Jacquelyn S. Fetrow Diana L. Schaak Steven C. Almo Dietrich Kraft Erwin Heberle-Bors R. Valenta 《Sexual plant reproduction》1998,11(4):183-191
Profilins are structurally well conserved low molecular weight (12–15 kDa) eukaryotic proteins which interact with a variety
of physiological ligands: (1) cytoskeletal components, e.g., actin; (2) polyphosphoinositides, e.g., phosphatidylinositol-4,5-bisphosphate;
(3) proline-rich proteins, e.g., formin homology proteins and vasodilatator-stimulated phosphoprotein. Profilins may thus
link the microfilament system with signal transduction pathways. Plant profilins have recently been shown to be highly crossreactive
allergens which bind to IgE antibodies of allergic patients and thus cause symptoms of type I allergy. We expressed and purified
from Escherichia coli profilins from birch pollen (Betula verrucosa), humans (Homo sapiens) and yeast (Schizosaccharomyces pombe) and demonstrated that each of these profilins is able to form stable homo- and heteropolymers via disulphide bonds in vitro.
Circular dichroism analysis of oxidized (polymeric) and reduced (monomeric) birch pollen profilin indicates that the two states
have similar secondary structures. Using 125I-labeled birch pollen, yeast and human profilin in overlay experiments, we showed that disulphide bond formation between
profilins can be disrupted under reducing conditions, while reduced as well as oxidized profilin states bind to actin and
profilin-specific antibodies. Exposure of profilin to oxidizing conditions, such as when pollen profilins are liberated on
the surface of the mucosa of atopic patients, may lead to profilin polymerization and thus contribute to the sensitization
capacity of profilin as an allergen.
Received: 25 February 1998 / Revision accepted: 12 May 1998 相似文献