Inducible sfi dependent division inhibition in Escherichia coli

Summary Brief exposure of an Escherichia coli tif lon strain to 40° results in subsequent prolonged inhibition of cell division (part of the SOS response), which is completely and specifically suppressed by sfiA and sfiB mutations. This sfi dependent division inhibition requires protein synthesis during the 40° incubation period, implying the existence of a tif-inducible protein which results in cell division arrest. sfi dependent division inhibition is also induced early during thymine starvation in tif ⁺ cells; at later times a sfi independent mechanism of division arrest is invoked as well.In lon mutants, known to lack a protease, the sfi dependent division inhibition is amplified, perhaps due to stabilization of the inducible protein involved in division arrest. In these strains the P1 lysogenization defect and the filamentation observed after a nutritional shift-up are sfi dependent, suggesting that P1 infection and nutritional shift-up may also induce the protein involved in division arrest. Bacteria are known to increase in size following a shift-up. Thus the latter observation suggests that the SOS response may be not only a last resort in time of distress but also a means permitting better adaptation of the cells to their environment.After five years of heroic struggle against cancer, Jacqueline George passed away 14 August 1979. Despite weakened health and debilitating therapy she continued to stimulate and participate in the work of the microbial genetics group which she had created     

Heterogeneity in β0 thalassemia from Algeria: Genetic,clinical and molecular studies

Summary Six Algerian patients with ⁰ thalassemia are presented, in addition to the two patients already reported (Godet et al., 1977). Family studies indicate that all the patients had homozygous thalassemia characterized by absence of globin chain synthesis in peripheral blood. The clinical severity varies from one family to the other and within the same family, from typical Cooley's anemia to thalassemia intermedia and appears to be related to the child death rate observed in each family. The / biosynthetic ratio was 0.36–0.40 in seven patients and 0.2 in the most seriously affected patient. The mRNA content in peripheral reticulocytes was less than 1.5% of mRNA in seven patients and 13.3% in one patient. These results indicate that Algerians homozygous for thalassemia are heterozygous at the clinical, biochemical and molecular levels.     

Ultrastructure and lipid composition of etioplasts in developing dark-grown wheat leaves

Changes in the ultrastructure and lipid composition of etioplasts have been evaluated in three regions from the base to the tip of 8-day-old darkgrown wheat leaves and in the upper-2/3 region of etiolated leaves of different ages. In developing darkgrown tissues, the main morphological changes that etioplasts undergo consist of an increase in the amount of thylakoïds which, in the most mature etioplasts, align in parallel arrays. Concomitantly, galactolipids and sulfolipid form an increasing proportion of the total lipids. Trans-3-hexadecenoic acid was not detectable in phosphatidylglycerol (PG) of etioplasts showing appressed thylakoïds isolated from 5-day-old leaves, but was present in significant amounts in etioplasts in the basal part of 8-day-ols leaves in which membrane appression was barely visible. The proportions of this acid increase as etioplasts develop, reaching 25% of the PG fatty acids (1.2% of the total fatty acids) in the most differentiated etioplasts. In wheat etioplasts, it appears that trans-3-hexadecenoic acid may accumulate in considerable amounts in darkgrown tissues and that its accumulation is not directly involved in membrane appression.Abbreviations AP phosphatidic acid - DGDG digalactosyldiacylglycerol - MGDG monogalactosyldiacylglycerol - PC phosphatidylcholine - PE phosphatidylethanolamine - PG phosphatidylglycerol - PI phosphatidylinositol - PS phosphatidylserine - SL sulfolipid     

Replication of Simian Virus 40 Deoxyribonucleic Acid: Analysis of the One-Step Growth Cycle

The time course of replication of simian virus 40 deoxyribonucleic acid (DNA) was investigated in growing monolayer cultures of subcloned CV1 cells. At multiplicities of infection of 30 to 60 plaque-forming units (PFU)/cell, first progeny DNA molecules (component 1) were detected by 10 hr after infection. During the following 10 to 12 hr, accumulation of virus DNA proceeded at ever increasing rates, albeit in a non-exponential fashion. The rate of synthesis then remained constant, until approximately the 40th hour postinfection, when DNA replication stopped. Under these conditions, the duration of the virus growth cycle was approximately 50 hr. The time needed for the synthesis of one DNA molecule was found to be approximately 15 min. At multiplicities of infection of 1 or less than 1 PFU/cell, the onset of the linear phase of DNA accumulation was delayed, but the final rate of DNA synthesis was the same, independent of the input multiplicity. This was taken as a proof that templates for the synthesis of viral DNA multiply in the cell during the early phase of replication. However, the probability for every replicated DNA molecule to become in turn replicative decreased constantly during that phase. This could be accounted for by assuming a limited number of replication sites in the infected cell.     

Evolution des cellules gonadotropes préhypophysaires au cours du cycle oestral chez la ratte

Résumé L'évolution des cellules gonadotropes préhypophysaires ß et a été étudiée au cours de cycles de 4 jours chez la ratte.Ces 2 catégories cellulaires subissent au cours du cycle ovarien des variations morphologiques et numériques qui paraissent témoigner d'une activité folliculostimulante pour les cellules ß et d'une activité ovulatoire pour les cellules .Les cellules ß se dégranulent une l^ère fois pendant la nuit du dioestrus II au prooestrus, puis une 2^e fois, lors de la phase préovulatoire, l'après-midi du prooestrus.Les cellules présentent, au cours de l'après-midi du prooestrus, une dégranulation massive, qui peut etre mise en rapport avec la décharge de L. H. qui se produit à ce stade du cycle.La l^ère dégranulation des cellules ß en dioestrus II correspondrait à la vague de croissance folliculaire qui, selon Long et Evans, a lieu à ce stade du cycle et mènerait certains follicules à la rupture. La seconde traduirait une décharge de FSH synergique de l'excrétion de L. H. par les cellules , pendant la phase préovulatoire du cycle ovarien.

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Summary Morphological and numerical variations of the pituitary gonadotrophs ß and were studied during 4 days cycles at different stages of the oestrous cycle in the Rat.The highest degree of degranulation of the ß cells was observed first during the night of dioestrous II to prooestrous and afterwards on the afternon of prooestrous.The degranulation of the cells began in the morning of prooestrous and reached its higher degree at 4 p. m. in prooestrous.The first peak of degranulation of the ß cells was supposed to be related to the wave of follicular growth which occurs on the day of dioestrous II according to Long and Evans. The second phase of degranulation of these cells was considered as a prooestrous diurnal discharge of FSH synergically with the ovulatory discharge of LH by the cells during the critical period of the cycle.

     

INCORPORATION OF TRITIATED THYMIDINE IN THE CELLS OF CAENORHABDITIS BRIGGSAE (NEMATODA) REARED IN AXENIC CULTURE

In the rhabditid nematode Caenorhabditis briggsae the incorporation of thymidine-H³ has been studied by autoradiography after Feulgen staining, with animals maintained under axenic conditions in a medium of only partly defined composition. Labeling has been followed in adults left in the presence of thymidine-H³ for periods of from ½ to 24 hours, as well as in adults reared from larvae in the presence of the tritiated nucleoside. A massive incorporation is found in the nuclei of the gonads and intestine; also a less intense particulate cytoplasmic incorporation is clear in certain cells, especially those of the intestine. In general, all labeling has proved to be sensitive to DNase, but resistant to RNase. The label's stability has been tested by the transfer of adults into a medium containing "cold" thymidine. They remain there for up to 48 hours. A transfer for 24 hours results in a considerable decrease in the intensity of nuclear and cytoplasmic labeling; a stay of 48 hours leads to its complete disappearance from non-dividing (intestinal) as well as dividing (gonadal) nuclei. A phenomenon of DNA turnover is envisaged and discussed as a possible physiological attribute of C. briggsae.     

Bdellovibrios in Callinectus sapidus, the Blue Crab

Bdellovibrios were recovered from the gill tissue of all of 31 crabs sampled and from all samples of epibiota obtained from the ventral shell surface of 15 crabs. The results suggest that the blue crab is a reservoir for bdellovibrios. The association with crabs may be an important factor in the ecology of the bdellovibrios.     

Genes encoding the small subunit of RUBISCO belong to two highly conserved subfamilies in Nicotianeae

Summary The sequences of seven complementary DNAs or genes encoding the small subunit (SSU) of ribulose-1,5-bisphosphate carboxylase oxygenase (RUBISCO) in several Nicotianeae were examined. Two new SSU genes isolated fromNicotiana sylvestris were included. Both sequence comparisons and Southern analyses with specific probes reveal that SSU genes fall into two homogeneous subfamilies that are highly conserved in Nicotianeae and are also present in other Solanaceae. Additional criteria such as number of introns and level of expression fitted to this classification. Homogeneity must have been maintained by gene conversion and/or an unusually high fidelity of DNA replication, whereas traces of slippage-stranded DNA mispairing and/or transposition probably explain local changes. Taken as a whole, these results show that the divergence between the two subfamilies predated the divergence between genera inside the Solanaceae, but that Nicotianeae retained the most simple SSU gene family structure.     

Declines in sociosexual behavior in golden monkeys (Rhinopithecus roxellana): Associated environmental and social variables

Between February 22 and August 10, 1986, 200 h of systematic observation were conducted on a male and female golden monkey (Rhinopithecus roxellana) at Seattle's Woodland Park Zoo and Portland's Washington Park Zoo. The pair was moved to Portland on May 6, and on July 24 the female unexpectedly gave birth to a male infant, the first such birth in the United States. Dramatic behavioral changes occurred immediately after the pair was moved to Portland, the midpoint of the female's pregnancy, including the disappearance of sexual behavior and a sharp decline in time spent in contact. Explanations for these changes are discussed.     

Chiral linear hydroxamates as biomimetic analogues of ferrioxamine and coprogen and their use in probing siderophore-receptor specificity in bacteria and fungi

Summary Linear hydroxamate derivatives, possessing chiral -amino acid moieties, were synthesized and their iron transport activities were studied in bacteria and fungi. No growth-promoting activity could be detected in the Gram-positive hydroxamate-auxotrophAureobacterium flavescens JG9. However, Gram-negative enterobacteria, such asEscherichia coli, Pantoea agglomerans andHafnia alvei were able to utilize iron from these analogues. Uptake of⁵⁵Fe-labeled analogues was inhibited by sodium azide, suggesting an active transport process. The receptors involved during uptake in enterobacteria were identified by using appropriate indicator organisms which are defective in the transport of either ferrioxamines (P. agglomerans FM13), coprogens (H. alvei), or both of these siderophore classes (E. coli fhuE). Our data suggest that the chiral hydroxamates are recognized by the ferrioxamine receptor (FoxA) and the coprogen receptor (FhuE) at a ratio which depends on the optical/ isomer fraction and the nature of side chains. Transport was also observed in the fungusNeurospora crassa, known to take up coprogen rather than ferrioxamines, suggesting that in this fungus the synthetic analogues behave like coprogen.