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991.
J L Pons N Bonnaveiro J Chevalier A Crémieux 《The Journal of applied bacteriology》1992,73(5):395-400
The antimicrobial interactions of 49 combinations of chlorhexidine, quaternary ammonium compounds, preservatives and excipients were evaluated by the method of Berenbaum and the checkerboard titration method, with Staphylococcus aureus CIP 53154 and Escherichia coli CIP 54127 as test strains. MIC determinations were carried out as a preliminary step, and relative growth intensity was used to describe the bacteriostatic activity of surface-active agents (Amonyl 380 BA, Amonyl 671 SB). In the study of combinations, results were interpreted with Fractional Inhibitory Concentration indexes and represented by isobolograms. A fair correlation was shown between the method of Berenbaum and the checkerboard titration method. Combinations between chlorhexidine, cetrimonium bromide and benzalkonium chloride were synergistic or additive; combinations of antiseptics and preservatives were generally not antagonistic. The methods were also well adapted to the study of interactions involving surface-active agents, a critical problem in the formulation of topical antimicrobial agents. 相似文献
992.
Kin Sing Lam Jeffrey A. Titus Thomas T. Dabrah Dennis L. Kimball Jacqueline M. Veitch Donald R. Gustavson Bruce J. Compton James A. Matson Salvatore Forenza John Ross David Miller John Roach John Beutler 《Journal of industrial microbiology & biotechnology》1992,11(1):7-12
Summary Supplementing the culture ofMicromonospora chersina sp. nov. No. M956-1 with NaI (0.5 mg/l) enhanced the production of dynemicin A by 35-fold in shake flask culture. Homogeneous dynemicin A was obtained from the whole broth extract by Dicalite chromatography, Sephadex LH-20 chromatography and vacuum liquid chromatography. Gram quantities of dynemicin A were obtained from the fermentation ofM. chersina sp. nov. No. M956-1 in a 10000-liter fermentor. 相似文献
993.
J.-L. Pons N. Bonnaveiro J. Chevalier A. Crémieux 《Journal of applied microbiology》1992,73(5):395-400
The antimicrobial interactions of 49 combinations of chlorhexidine, quaternary ammonium compounds, preservatives and excipients were evaluated by the method of Berenbaum and the checkerboard titration method, with Staphylococcus aureus CIP 53154 and Escherichia coli CIP 54127 as test strains. MIC determinations were carried out as a preliminary step, and relative growth intensity was used to describe the bacteriostatic activity of surface-active agents (Amonyl 380 BA®, Amonyl 671 SB®). In the study of combinations, results were interpreted with Fractional Inhibitory Concentration indexes and represented by isobolograms. A fair correlation was shown between the method of Berenbaum and the checkerboard titration method. Combinations between chlorhexidine, cetrimonium bromide and benzalkonium chloride were synergistic or additive; combinations of antiseptics and preservatives were generally not antagonistic. The methods were also well adapted to the study of interactions involving surface-active agents, a critical problem in the formulation of topical antimicrobial agents. 相似文献
994.
Glover JJ 《Theoretical medicine》1992,13(4):293-294
Does the care perspective make a difference? Can it reach as far as we would like? It is the goal of this special issue on Gender in Bioethics: Theory and Practice to begin to address some of these important questions. In the first article, Virginia Sharpe provides a comprehensive and thoughtful analysis of how the orientations of justice and care are played out in medical ethical theory. In the second article, James Nelson argues that the more traditional approach in Bioethics to maternal-fetal conflicts is not useful, and that a care perspective is more appropriate. Thoughtful commentary on Nelson's article is provided by Rosemarie Tong. For readers new to the justice-care debate in moral theory, the following articles will provide an informative introduction. For those who are more familiar, it is hoped that you will be challenged by the extension of the debate to the practical issue of maternal-fetal conflicts. 相似文献
995.
Shu-Ren Wang Guy Renaud Jacqueline Infante Danièle Catala Recaredo Infante 《In vitro cellular & developmental biology. Plant》1985,21(9):526-530
Summary Isolated hepatocytes from adult rat liver were prepared after dissociation of the liver with EDTA. The morphological appearance,
viability (94.5%) and yield (1.76.107 cells/g liver) compare well with those of previously described methods using collagenase. Differentiated functions of the
hepatocytes in primary culture such as albumin secretion (10.9 μg/mg cell protein/d) and triglyceride synthesis and secretion
are maintained. Induction of triglyceride synthesis and secretion by oleic acid takes place to an extent similar to that observed
in vivo and liver perfusion. Particles with a lipid composition resembling circulating very low density lipoproteins are secreted
into the medium. These characteristics demonstrate the ability of hepatocytes isolated with EDTA and subsequently used in
primary culture to retain complex and highly differentiated functions of the intact liver. 相似文献
996.
A type-III group-B streptococcus (Streptococcus agalactiae) isolated from a case of late-onset sepsis was examined for protease production. In broth culture, extracellular proteolytic enzymes were not detected until the late exponential phase of growth with maximal protease production occurring during the stationary phase. Three distinct protease pools were isolated from the supernatant fluids of stationary-phase cultures, employing a combination of ion-exchange chromatography and gel-filtration chromatography. One population of proteases (containing two protease pools separable by gel filtration chromatography) eluted from a diethylaminoethyl cellulose column at a sodium chloride gradient concentration of 0.15M while a second population eluted from the same material at a sodium chloride concentration of 0.35M. These protease pools varied in molecular weights from approximately 25,000 daltons to 160,000 daltons as determined by gel filtration on Sephadex G-200. All three protease preparations had pH optima of 8.0–9.0, and all were active against gelatin, human serum albumin, and casein, but were not active against elastin or collagen. In addition, all three protease preparations completely inactivated purified type-III group-B streptococal neuraminidase. The role of these proteases in the disease process caused by the type-III group-B streptococci must remain speculative at this time. 相似文献
997.
Suspension cultured Nicotiana tabacum (tobacco) cells grow slowly on intermediates of the purine degradation pathway (hypoxanthine, xanthine, uric acid, allantoin, and urea) as their sole nitrogen source indicating that this degradation pathway is operative in these cells. The hypoxanthine analog, allopurinol inhibited tobacco cell growth on hypoxanthine but not uric acid. This helps confirm that the site of action of allopurinol is the conversion of hypoxanthine to uric acid by xanthine oxidase. Attempts to select cells which could grow in the presence of allopurinol with hypoxanthine as the nitrogen source were not successful. 相似文献
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