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991.
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An examination of the ontogeny of the lateral line trunk canal and the diversity of adult trunk canal patterns among cichlids indicates that bidirectional canal formation is a general ontogenetic pattern in the Cichlidae with the exception of Cichla and those few species with a complete trunk canal pattern. In addition to the tubed scales which make up the trunk canal, some lateral line scales have pits containing superficial neuromasts. These are recognized as components of the lateral line system of the trunk in adult cichlids for the first time. Eight trunk canal patterns that are variations on a simple disjunct pattern are defined among the 17 cichlid genera examined. Using bidirectional canal formation as a developmental model, these patterns can be placed along an ontogenetic spectrum. This suggests that heterochrony (alterations in the timing of development) is an important mechanism of evolutionary change in the lateral line system of the trunk in cichlid fishes. 相似文献
994.
Tazzio Tissot Audrey Arnal Camille Jacqueline Robert Poulin Thierry Lefèvre Frédéric Mery François Renaud Benjamin Roche François Massol Michel Salzet Paul Ewald Aurélie Tasiemski Beata Ujvari Frédéric Thomas 《BioEssays : news and reviews in molecular, cellular and developmental biology》2016,38(3):276-285
995.
Post‐embryonic development of canal and superficial neuromasts and the generation of two cranial lateral line phenotypes 下载免费PDF全文
The relatively simple structural organization of the cranial lateral line system of bony fishes provides a valuable context in which to explore the ways in which variation in post‐embryonic development results in functionally distinct phenotypes, thus providing a link between development, evolution, and behavior. Vital fluorescent staining, histology, and scanning electron microscopy were used to describe the distribution, morphology, and ontogeny of the canal and superficial neuromasts on the head of two Lake Malawi cichlids with contrasting lateral line canal phenotypes (Tramitichromis sp. [narrow‐simple, well‐ossified canals with small pores] and Aulonocara stuartgranti [widened, more weakly ossified canals with large pores]). This work showed that: 1) the patterning (number, distribution) of canal neuromasts, and the process of canal morphogenesis typical of bony fishes was the same in the two species, 2) two sub‐populations of neuromasts (presumptive canal neuromasts and superficial neuromasts) are already distinguishable in small larvae and demonstrate distinctive ontogenetic trajectories in both species, 3) canal neuromasts differ with respect to ontogenetic trends in size and proportions between canals and between species, 4) the size, shape, configuration, physiological orientation, and overall rate of proliferation varies among the nine series of superficial neuromasts, which are found in both species, and 5) in Aulonocara, in particular, a consistent number of canal neuromasts accompanied by variability in the formation of canal pores during canal morphogenesis demonstrates independence of early and late phases of lateral line development. This work provides a new perspective on the contributions of post‐embryonic phases of lateral line development and to the generation of distinct phenotypes in the lateral line system of bony fishes. J. Morphol. 277:1273–1291, 2016. © 2016 Wiley Periodicals, Inc. 相似文献
996.
Jacqueline M. Doyle Todd E. Katzner Gary W. Roemer James W. CainIII Brian A. Millsap Carol L. McIntyre Sarah A. Sonsthagen Nadia B. Fernandez Maria Wheeler Zafer Bulut Peter H. Bloom J. Andrew DeWoody 《Conservation Genetics》2016,17(6):1307-1322
Molecular markers can reveal interesting aspects of organismal ecology and evolution, especially when surveyed in rare or elusive species. Herein, we provide a preliminary assessment of golden eagle (Aquila chrysaetos) population structure in North America using novel single nucleotide polymorphisms (SNPs). These SNPs included one molecular sexing marker, two mitochondrial markers, 85 putatively neutral markers that were derived from noncoding regions within large intergenic intervals, and 74 putatively nonneutral markers found in or very near protein-coding genes. We genotyped 523 eagle samples at these 162 SNPs and quantified genotyping error rates and variability at each marker. Our samples corresponded to 344 individual golden eagles as assessed by unique multilocus genotypes. Observed heterozygosity of known adults was significantly higher than of chicks, as was the number of heterozygous loci, indicating that mean zygosity measured across all 159 autosomal markers was an indicator of fitness as it is associated with eagle survival to adulthood. Finally, we used chick samples of known provenance to test for population differentiation across portions of North America and found pronounced structure among geographic sampling sites. These data indicate that cryptic genetic population structure is likely widespread in the golden eagle gene pool, and that extensive field sampling and genotyping will be required to more clearly delineate management units within North America and elsewhere. 相似文献
997.
Franklin BS Rodrigues SO Antonelli LR Oliveira RV Goncalves AM Sales-Junior PA Valente EP Alvarez-Leite JI Ropert C Golenbock DT Gazzinelli RT 《Microbes and infection / Institut Pasteur》2007,9(7):881-890
We investigated the role of different TLRs and MyD88 in host resistance to infection and malaria pathogenesis. TLR2(-/-), TLR4(-/-), TLR6(-/-), TLR9(-/-) or CD14(-/-) mice showed no change in phenotypes (parasitemia, body weight and temperature) when infected with Plasmodium chabaudi chabaudi (AS). MyD88(-/-) mice displayed comparable ability to wild type animals in controlling and clearing parasitemia. Importantly, MyD88(-/-) mice exhibited impaired production of TNF-alpha and IFN-gamma as well as attenuated symptoms, as indicated by changes in body weight and temperature during parasitemia. Consistently, CD11b(+) monocytes and CD11c(+) dendritic cells from infected MyD88(-/-) mice were shown impaired for production of pro-inflammatory cytokines, and in initiating CD4(+) T cell responses. Importantly, the inhibition of T cell activation with anti-CD134L, mostly inhibited IFN-gamma, partially inhibited TNF-alpha production, and protected the animals from malaria symptoms. Our findings suggest that MyD88 and possibly its associated TLRs expressed by dendritic cells play an important role in pro-inflammatory responses, T cell activation, and pathogenesis of malaria, but are not critical for the immunological control of the erythrocytic stage of P. chabaudi. 相似文献
998.
Chen CY Scurrah KJ Stankovich J Garoufalis P Dirani M Pertile KK Richardson AJ Mitchell P Baird PN 《Human genetics》2007,121(3-4):511-520
To examine the familial correlations, heritability (h
2) and common environmental components (c
2) of myopia and ocular biometric traits (all treated as continuous outcomes) in families collected through the Genes in Myopia
(GEM) family study in Australia. A total of 132 pedigrees (723 participants) were recruited for this study. All individuals
completed a risk factor questionnaire and underwent a detailed eye examination including spherical equivalent (SphE) and ocular
biometric measurements of axial length (AL), anterior chamber depth (ACD) and corneal curvature (CC). Familial correlations
were calculated and h
2 and c2 were estimated using a variance component model that assumes a multivariate t distribution within each pedigree. Two definitions
of common environments (c
2) were considered: nuclear family (current) shared environment (Model 1) and sib-ship (childhood) shared environment (Model
2). Population ascertainment adjustment was performed using the Blue Mountains eye study dataset. The trends observed for
familial correlations suggested that SphE is influenced by both environmental and genetic factors whereas AL, ACD and CC are
predominantly genetically determined. This was largely confirmed by variance components modelling. Heritability estimates
(adjusted for age, sex and years of education) from the best fitting ACE model (Model 2, childhood shared environment) were
0.50 ± 0.05 for SphE, 0.73 ± 0.04 for AL, 0.78 ± 0.04 for ACD and 0.16 ± 0.06 for CC. Childhood environmental effects were
significant with c
2 estimated to be 0.33 ± 0.04 for SphE, 0.06 ± 0.03 for AL, 0.22 ± 0.04 for ACD and 0.10 ± 0.05 for CC. Age was associated
with SphE, total years of education was associated with AL and sex was associated with all traits studied. We used a novel
and conservative approach to account for and estimate common environmental effects by specifying either nuclear family or
sib-ship environment when estimating heritability estimates and showed that all traits examined (SphE, AL, ACD and CC) are
heritable, thus reflecting a genetic component. These traits therefore all represent candidates for quantitative trait linkage
analyses. 相似文献
999.
Genotyping‐by‐sequencing approaches to characterize crop genomes: choosing the right tool for the right application 下载免费PDF全文
In the last decade, the revolution in sequencing technologies has deeply impacted crop genotyping practice. New methods allowing rapid, high‐throughput genotyping of entire crop populations have proliferated and opened the door to wider use of molecular tools in plant breeding. These new genotyping‐by‐sequencing (GBS) methods include over a dozen reduced‐representation sequencing (RRS) approaches and at least four whole‐genome resequencing (WGR) approaches. The diversity of methods available, each often producing different types of data at different cost, can make selection of the best‐suited method seem a daunting task. We review the most common genotyping methods used today and compare their suitability for linkage mapping, genomewide association studies (GWAS), marker‐assisted and genomic selection and genome assembly and improvement in crops with various genome sizes and complexity. Furthermore, we give an outline of bioinformatics tools for analysis of genotyping data. WGR is well suited to genotyping biparental cross populations with complex, small‐ to moderate‐sized genomes and provides the lowest cost per marker data point. RRS approaches differ in their suitability for various tasks, but demonstrate similar costs per marker data point. These approaches are generally better suited for de novo applications and more cost‐effective when genotyping populations with large genomes or high heterozygosity. We expect that although RRS approaches will remain the most cost‐effective for some time, WGR will become more widespread for crop genotyping as sequencing costs continue to decrease. 相似文献
1000.
Jacqueline M. Skeer David B. Sattelle 《Archives of insect biochemistry and physiology》1993,23(3):111-124
This study has identified specific, stereoselective phenylalkylamine (PAA, (±)- [3H]verapamil) binding sites of low-affinity and high-density in cockroach (Periplaneta americana) nervous system and skeletal muscle membranes. Scatchard transformation of equilibrium binding data revealed a single population of binding sites in both tissues with dissociation constants (Kd) of 273 nM and 377 nM and binding capacities (Bmax) of 23 pmol·mg protein?1 and 37pmol·mg protein?1 for cockroach nervous tissue and skeletal muscle membranes, respectively. The PAA binding site in cockroach nervous tissue membranes was found to be dihydropyridine (DHP)-insensitive, whereas the corresponding site in cockroach skeletal muscle membranes was DHP-sensitive. This property of a DHP-sensitive PAA receptor distinguishes the binding sites identified in cockroach skeletal muscle from those in cockroach nervous tissue and indicates that pharmacologically distinct putative Ca2+ channel subtypes are present in insect nerve and muscle. © 1993 Wiley-Liss, Inc. 相似文献