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991.
Rmy Fortrie David Gatineau Damien Hrault Aurlie Bal Jean-Valre Naubron Laurent Giordano Grard Buono 《Chirality》2022,34(1):27-33
The racemization mechanism of tert-butylphenylphosphido-borane is investigated experimentally and theoretically. Based on this converging approach, it is shown, first, that several phosphido-borane molecular species coexist at the time of the reaction and, second, that one particular of both initially assumed reactive routes most significantly contribute to the overall racemization process. From our converging modeling and experimental measurement, it comes out that the most probable species to be here encountered is a phosphido-borane-Li (THF)2 neutral solvate, whose P-stereogenic center monomolecular inversion through a Y-shaped transition structure (ΔrG°≠: 81 kJ mol?1) brings the largest contribution to the racemization process. 相似文献
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María?Valladares María?Reyes-Batlle Carmen?M.?Martín-Navarro Atteneri?López-Arencibia Alexis?Dorta-Gorrín Carolina?Wagner Enrique?Martínez-Carretero José?E.?Pi?ero Basilio?Valladares Jacob?Lorenzo-MoralesEmail author 《Archives of microbiology》2015,197(5):639-643
The present study describes two cases of Acanthamoeba infections (keratitis and ascites/peritonitis) in small breed domestic dogs in Tenerife, Canary Islands, Spain. In both cases, amoebic trophozoites were observed under the inverted microscope and isolated from the infected tissues and/or fluids, without detecting the presence of other viral, fungal or bacterial pathogens. Amoebae were isolated using 2 % non-nutrient agar plates and axenified for further biochemical and molecular analyses. Osmotolerance and thermotolerance assays revealed that both isolates were able to grow up to 37 °C and 1 M of mannitol and were thus considered as potentially pathogenic. Moreover, the strains were classified as highly cytotoxic as they cause more than 75 % of toxicity when incubated with two eukaryotic cell lines. In order to classify the strains at the molecular level, the diagnostic fragment 3 (DF3) region of the 18S rDNA of Acanthamoeba was amplified and sequenced, revealing that both isolates belonged to genotype T4. In both cases, owners of the animals did not allow any further studies or follow-up and therefore the current status of these animals is unknown. Furthermore, the isolation of these pathogenic amoebae should raise awareness with the veterinary community locally and worldwide. 相似文献
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Zhun Mao Laurent Saint-André Franck Bourrier Alexia Stokes Thomas Cordonnier 《Annals of botany》2015,116(2):261-277
Background and Aims In mountain ecosystems, predicting root density in three dimensions (3-D) is highly challenging due to the spatial heterogeneity of forest communities. This study presents a simple and semi-mechanistic model, named ChaMRoots, that predicts root interception density (RID, number of roots m–2). ChaMRoots hypothesizes that RID at a given point is affected by the presence of roots from surrounding trees forming a polygon shape.Methods The model comprises three sub-models for predicting: (1) the spatial heterogeneity – RID of the finest roots in the top soil layer as a function of tree basal area at breast height, and the distance between the tree and a given point; (2) the diameter spectrum – the distribution of RID as a function of root diameter up to 50 mm thick; and (3) the vertical profile – the distribution of RID as a function of soil depth. The RID data used for fitting in the model were measured in two uneven-aged mountain forest ecosystems in the French Alps. These sites differ in tree density and species composition.Key Results In general, the validation of each sub-model indicated that all sub-models of ChaMRoots had good fits. The model achieved a highly satisfactory compromise between the number of aerial input parameters and the fit to the observed data.Conclusions The semi-mechanistic ChaMRoots model focuses on the spatial distribution of root density at the tree cluster scale, in contrast to the majority of published root models, which function at the level of the individual. Based on easy-to-measure characteristics, simple forest inventory protocols and three sub-models, it achieves a good compromise between the complexity of the case study area and that of the global model structure. ChaMRoots can be easily coupled with spatially explicit individual-based forest dynamics models and thus provides a highly transferable approach for modelling 3-D root spatial distribution in complex forest ecosystems. 相似文献
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ETHYLENE RESPONSE FACTOR 96 positively regulates Arabidopsis resistance to necrotrophic pathogens by direct binding to GCC elements of jasmonate – and ethylene‐responsive defence genes
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Holger Maier Christine Schütt Ralph Steinkamp Anja Hurt Elida Schneltzer Philipp Gormanns Christoph Lengger Mark Griffiths David Melvin Neha Agrawal Rafael Alcantara Arthur Evans David Gannon Simon Holroyd Christian Kipp Navis Pretheeba Raj David Richardson Sophie LeBlanc Laurent Vasseur Hiroshi Masuya Kimio Kobayashi Tomohiro Suzuki Nobuhiko Tanaka Shigeharu Wakana Alison Walling David Clary Juan Gallegos Helmut Fuchs Martin Hrabě de Angelis Valerie Gailus-Durner 《Mammalian genome》2015,26(9-10):467-481