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991.
Jack Rosenbluth 《The Journal of cell biology》1971,50(3):900-904
992.
In order to show whether the estrogen complex (ER) in the intact cell binds to some nuclear component or whether it is in free equilibrium between the cytoplasm and the nucleus, we incubated intact uteri under conditions which increased the ratio of nuclear to cytoplasmic ER. These conditions included (a) the use of sucrose at concentrations greater than 0.75 M, (b) ethanol at 7.5% to 10%, or (c) 1 mM mercuric chloride or phenylmercuric acetate. Whereas (b) and (c) increased the ratio by preferentially denaturing the cytoplasmic ER, (a) caused ER to move from the cytoplasm into the nucleus by an undetermined mechanism. Uteri with a high ratio of nuclear to cytoplasmic ER were then washed, incubated in fresh “normal” incubation media, fractionated and the location of ER determined. If ER binding does occur in the nucleus, the high ratio of nuclear ER to cytoplasmic ER should be maintained, whereas if ER is in an equilibrium in the cell, ER should redistribute and reestablish the “normal” ratio. In all cases studied; i.e., after pretreatment with sucrose at different concentrations, ethanol at different concentrations and either mercuric chloride or phenylmercuric acetate, the ratio of nuclear to cytoplasmic ER remained high, suggesting that ER binds to some nuclear component in intact cells. 相似文献
993.
A programme for specialized training in family medicine at Tel Aviv University Medical School provides four years of postgraduate study, two of which are in recognized hospital departments and two in clinics recognized for training in family medicine. At the end of this four-year period the graduate must submit a thesis of original work or an approved research project on an aspect of family medicine. Continuing contact of the trainee with both the family medicine clinic and the hospital departments is maintained throughout the period. While in hospital the trainee spends half a day a week in the family clinic with his personal tutor, and when in the clinic he spends half a day a week in the hospital.This programme has been provisionally accepted as meeting the formal requirements of the Israel Medical Association for specialization in family medicine, and the first physician has started training. 相似文献
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