全文获取类型
收费全文 | 70篇 |
免费 | 2篇 |
出版年
2020年 | 1篇 |
2017年 | 1篇 |
2015年 | 2篇 |
2013年 | 1篇 |
2011年 | 2篇 |
2010年 | 3篇 |
2009年 | 5篇 |
2005年 | 4篇 |
2004年 | 1篇 |
2003年 | 5篇 |
2002年 | 5篇 |
2001年 | 1篇 |
2000年 | 4篇 |
1999年 | 1篇 |
1998年 | 5篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1993年 | 3篇 |
1992年 | 4篇 |
1991年 | 2篇 |
1989年 | 2篇 |
1988年 | 3篇 |
1987年 | 1篇 |
1986年 | 2篇 |
1985年 | 3篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1978年 | 1篇 |
1973年 | 3篇 |
1972年 | 1篇 |
排序方式: 共有72条查询结果,搜索用时 15 毫秒
61.
Complex I (NADH-ubiquinone reductase) is a complex system located in the inner mitochondrial membrane and has the ability to catalyse several different enzymatic reactions concerned in electron transport. It is known to be one of the first components of the respiratory chain to be damaged by ischemia. Our results, using autolysis in the rat heart as experimental model, indicate that the NADH dehydrogenase system was impaired relatively early during ischemia while transhydrogenation and NADPH dehydrogenation appeared to be relatively resistant. 相似文献
62.
Two abnormal thyroglobulin-like polypeptides are produced from Afrikander cattle congenital goiter mRNA 总被引:2,自引:0,他引:2
V P Tassi R Di Lauro P Van Jaarsveld C G Alvino 《The Journal of biological chemistry》1984,259(16):10507-10510
In vitro translation of the mRNA from the congenital goiter tissue of Afrikander cattle directs the synthesis of two (Mr congruent to 250,000 and congruent to 75,000, respectively) abnormal thyroglobulin-like polypeptides. Common features of these polypeptides are the following: 1) both are immunoprecipitated by purified, anti-thyroglobulin antibody; 2) the signal sequence is present on their nascent polypeptide chains, and 3) their synthesis is apparently directed by the same high molecular weight mRNA. However, the goiter thyroglobulin mRNA shows, by Northern blot analysis, a slightly reduced size (congruent to 30 S) as compared to the mRNA from normal, bovine, thyroid tissue (33 S). Furthermore, a 10- to 15-fold decrease (as assessed by dot blot hybridization) in relative mRNA concentration was observed. Thus, it can be concluded that the genetic defect in congenital goiter of Afrikander cattle results in the synthesis of an altered mRNA which is present in decreased amount and directs the synthesis of two abnormal thyroglobulin-like polypeptides. 相似文献
63.
Priority areas for the conservation of South African vegetation: a coarse-filter approach 总被引:1,自引:0,他引:1
B. Reyers D. H. K. Fairbanks A. S. Van Jaarsveld M. Thompson 《Diversity & distributions》2001,7(1-2):79-95
Abstract. South Africa has an important responsibility to global biodiversity conservation, but a largely inadequate conservation area network for addressing this responsibility. This study employs a coarse-filter approach based on 68 potential vegetation units to identify areas that are largely transformed, degraded or impacted upon by road-effects. The assessment highlights broad vegetation types that face high biodiversity losses currently or in the near future due to human impacts. Most vegetation types contain large tracts of natural vegetation, with little degradation, transformation or impacts from road networks. Regions in the grasslands, fynbos and forest biomes are worst affected. Very few of the vegetation types are adequately protected according to the IUCN's 10% protected area conservation target, with the fynbos and savanna biomes containing a few vegetation types that do achieve this arbitrary goal. This investigation identifies areas where limited conservation resources should be concentrated by identifying vegetation types with high levels of anthropogenic land use threats and associated current and potential biodiversity loss. 相似文献
64.
65.
The method described for the isolation of mitochondrial complex I (NADH-ubiquinone reductase) from bovine hearts could not be applied to donkey hearts as unacceptably large losses in enzyme activity occurred. This method was modified for the isolation of complex I using donkey hearts and two complexes were obtained: complex IA which was physiologically inactive and complex IB which was physiologically active as it catalyzed the reaction from NADH to ubiquinone. Both complexes had relatively low enzyme activity with artificial electron acceptors, except with potassium ferricyanide, and had more or less the same amount of acid-labile sulfur and nonheme iron although the polypeptide composition differed to a great extent. 相似文献
66.
67.
H. Van Jaarsveld J. M. Kuyl E. H. De Wet D. W. Alberts F. D. Van Der Westhuizen 《Free radical research》1991,15(3):151-157
Anaesthetic drugs can induce reversible as well as irreversible changes in cell membranes and intracellular proteins as well as lipid peroxidation in the liver. Low molecular weight iron species (LMWI) can by their catalytic activity contribute to the generation of free radicals (hydroxyl radicals). Free radicals are a recognisable cause of intracellular damage. Impaired mitochondrial function is also a sign of intracellular damage, which is usually irreversible. Thus, an agent may be cytotoxic when it causes a significant increase in intracellular LMWI. Whether the LMWI arise from ferritin or is released from iron containing proteins, the same reaction occurs. As long as LMWI can undergo redox cycling, hydroxyl radicals can be formed. We investigated the effect of various mixtures of diethylether, halothane, nitrous oxide and oxygen on the intracellular LMWI content and mitochondrial function of the rat myocardium.
Hearts isolated from rats anaesthetised with diethylether showed an increase in the cytosolic LMWI compared to the control group. No increase in mitochondrial LMWI was demonstrated. Subsequent perfusion of the isolated hearts showed a further increase in the LMWI. On perfusion the mitochondrial LMWI increased in comparison with controls. Mitochondrial function was significantly impaired as measured by the QO2 (state 3), ADP/O ratio and oxidative phosphorylation rate (OPR).
Exposure of rats to 50% nitrous oxide for 15 minutes increased the myocardial LMWI, but had no effect on mitochondrial function. Exposure to room air for 30 minutes before isolating the hearts, still showed a significant increase in LMWI with no detectable change in mitochondrial function.
Halothane, on the other hand, did not have an effect on the myocardial LMWI and mitochondrial function in the experiment setup used. We therefore concluded that diethylether and nitrous oxide are potentially toxic to the myocardium and may potentiate the action of free radicals. 相似文献
Hearts isolated from rats anaesthetised with diethylether showed an increase in the cytosolic LMWI compared to the control group. No increase in mitochondrial LMWI was demonstrated. Subsequent perfusion of the isolated hearts showed a further increase in the LMWI. On perfusion the mitochondrial LMWI increased in comparison with controls. Mitochondrial function was significantly impaired as measured by the QO2 (state 3), ADP/O ratio and oxidative phosphorylation rate (OPR).
Exposure of rats to 50% nitrous oxide for 15 minutes increased the myocardial LMWI, but had no effect on mitochondrial function. Exposure to room air for 30 minutes before isolating the hearts, still showed a significant increase in LMWI with no detectable change in mitochondrial function.
Halothane, on the other hand, did not have an effect on the myocardial LMWI and mitochondrial function in the experiment setup used. We therefore concluded that diethylether and nitrous oxide are potentially toxic to the myocardium and may potentiate the action of free radicals. 相似文献
68.
Stefanie Freitag A. O. Nicholls A. S. van Jaarsveld 《Biodiversity and Conservation》1996,5(6):685-698
Iterative reserve selection algorithms were applied to two mammal databases, generalized to sixteenth degree grid squares, for the Transvaal region of South Africa. Based on primary point data, 24 grid squares are required to represent all species at least once, while only 13 grid squares are required when based on distribution map data; only two of these grid squares are common to both analyses. As the number of representations per species is increased from one to five, the number of selected grid squares increased to 86 and 71 or 72 respectively, with only 17 of these common to both analyses. These differences in the selection of sites are further reflected in the degree of congruence between selected grid squares and existing conservation areas which is on average 63.3% for grid squares selected from the primary database and only 42.5% for those selected from the distribution map database. These results emphasize the importance of quality data input when evaluating regional reserve networks. Highly generalized distribution map data sets, on the one hand, are extrapolations of limited data sets and contain non-quantifiable levels of false-positives which could have significant implications if used for establishing regional reserve networks. On the other hand, although there are problems associated with the establishment of primary diversity databases, namely data currency and uneven and non-random sampling (leading to false negatives), they remain our most reliable option for assigning conservation value. 相似文献
69.
70.
A S van Jaarsveld A A McKenzie J D Skinner 《Journal of reproduction and fertility》1992,95(3):765-773
A homologous radioimmunoassay system developed for humans was used to measure prolactin concentrations in spotted hyaenas. Concentrations of prolactin showed a significant (P < 0.05) decrease in lactating females, which is consistent with the infrequent suckling pattern of this species. This lack of hyperprolactinaemic conditions during lactation may explain the ability of females to resume reproductive activity soon after the loss of a litter, or even during lactation. Prolactin concentrations did not increase significantly during dispersion in male spotted hyaenas. This conforms to the pattern observed for cortisol, but differs from that for androgen, which fluctuates significantly with social suppression. Although comparative data from other species provide some circumstantial evidence for hyperprolactinaemic conditions during male dispersal, no obvious deductions regarding the recorded inverse relationship between prolactin and cortisol concentrations in mature males could be made. 相似文献