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931.
932.
Brad S. Coates Holly Johnson Kyung‐Seok Kim Richard L. Hellmich Craig A. Abel Charles Mason Thomas W. Sappington 《Ecology and evolution》2013,3(8):2459-2470
Female European corn borer, Ostrinia nubilalis, produce and males respond to sex pheromone blends with either E‐ or Z‐Δ11‐tetradecenyl acetate as the major component. E‐ and Z‐race populations are sympatric in the Eastern United States, Southeastern Canada, and the Mediterranean region of Europe. The E‐ and Z‐pheromone races of O. nubilalis are models for incipient species formation, but hybridization frequencies within natural populations remain obscure due to lack of a high‐throughput phenotyping method. Lassance et al. previously identified a pheromone gland‐expressed fatty‐acyl reductase gene (pgfar) that controls the ratio of Δ11‐tetradecenyl acetate stereoisomers. We identified three single nucleotide polymorphism (SNP) markers within pgfar that are differentially fixed between E‐ and Z‐race females, and that are ≥98.2% correlated with female pheromone ratios measured by gas chromatography. Genotypic data from locations in the United States demonstrated that pgfar‐z alleles were fixed within historically allopatric Z‐pheromone race populations in the Midwest, and that hybrid frequency ranged from 0.00 to 0.42 within 11 sympatric sites where the two races co‐occur in the Eastern United States (mean hybridization frequency or heterozygosity (HO) = 0.226 ± 0.279). Estimates of hybridization between the E‐ and Z‐races are important for understanding the dynamics involved in maintaining race integrity, and are consistent with previous estimates of low levels of genetic divergence between E‐ and Z‐races and the presence of weak prezygotic mating barriers. 相似文献
933.
The prepupation caterpillar of the Southeast Asian moth Calindoea trifascialis constructs a leaf shelter that jumps across the ground using a jumping method novel among the insects. We found that movement path direction was correlated to the direction opposite to the most intense light. Correlated random walk (CRW) analyses found net squared displacements higher than predicted by a CRW, and fractal dimension analysis indicated straighter paths at large spatial scales. Rearing experiments showed high mortality from predation on the ground, but higher mortality resulted from sun exposure. We interpret jumping path orientation as an efficient search strategy to find shade in a variable landscape, given limited perception, in the presence of overheating and desiccation risks. 相似文献
934.
935.
936.
Kim M Handley Nathan C VerBerkmoes Carl I Steefel Kenneth H Williams Itai Sharon Christopher S Miller Kyle R Frischkorn Karuna Chourey Brian C Thomas Manesh B Shah Philip E Long Robert L Hettich Jillian F Banfield 《The ISME journal》2013,7(4):800-816
Stimulation of subsurface microorganisms to induce reductive immobilization of metals is
a promising approach for bioremediation, yet the overall microbial community response is
typically poorly understood. Here we used proteogenomics to test the hypothesis that
excess input of acetate activates complex community functioning and syntrophic
interactions among autotrophs and heterotrophs. A flow-through sediment column was
incubated in a groundwater well of an acetate-amended aquifer and recovered during
microbial sulfate reduction. De novo reconstruction of community sequences
yielded near-complete genomes of Desulfobacter (Deltaproteobacteria),
Sulfurovum- and Sulfurimonas-like Epsilonproteobacteria and
Bacteroidetes. Partial genomes were obtained for Clostridiales
(Firmicutes) and Desulfuromonadales-like Deltaproteobacteria.
The majority of proteins identified by mass spectrometry corresponded to
Desulfobacter-like species, and demonstrate the role of this organism in
sulfate reduction (Dsr and APS), nitrogen fixation and acetate oxidation to CO2
during amendment. Results indicate less abundant Desulfuromonadales, and possibly
Bacteroidetes, also actively contributed to CO2 production via the
tricarboxylic acid (TCA) cycle. Proteomic data indicate that sulfide was partially
re-oxidized by Epsilonproteobacteria through nitrate-dependent sulfide oxidation
(using Nap, Nir, Nos, SQR and Sox), with CO2 fixed using the reverse TCA cycle.
We infer that high acetate concentrations, aimed at stimulating anaerobic heterotrophy,
led to the co-enrichment of, and carbon fixation in Epsilonproteobacteria.
Results give an insight into ecosystem behavior following addition of simple organic
carbon to the subsurface, and demonstrate a range of biological processes and community
interactions were stimulated. 相似文献
937.
Jeong Kyu Kim 《Entomological Research》2013,43(1):1-11
A total of 107 forms of 88 eumenine wasp species occurring in the Far East were used to produce a more comprehensive picture of the geographical pattern of wasps in the Far East. All forms occurring in the Far East were biogeographically classified into 22 types in four larger categories, and a summarized table and discussion are presented. The eumenine wasp fauna of the Far East was characterized as a mixture of a fair number of endemic elements, a moderate number of transpalearctic elements, and some Oriental elements. The endemism of the Far East reached 0.71 at the species/subspecies level when the calculation was based on true Palearctic elements occurring in Far Eastern Russia, the Korean peninsula and Japan proper. The relationship between the Far Eastern fauna and the Central/Western Palearctic faunas was also analyzed and discussed. The species occurring in each treated district was counted, and provides evidence for the importance of the Korean fauna in biodiversity evaluation/estimation of Far Eastern fauna. The similarity index between the Korean Peninsula and Japan proper, the Korean Peninsula and the Russian Far East, and the Russian Far East and Japan proper was calculated to explain possible factors for faunal formation in the Far East. The distributional pattern of each form treated in this study and its relatives is tabulated in Appendix I. 相似文献
938.
Bayu?Sisay?Tiruneh Byung-Hoon?Kim Daniel?R?Gallie Bijoyita?Roy Albrecht?G?von ArnimEmail author 《BMC biology》2013,11(1):123
Background
Genome-wide assays performed in Arabidopsis and other organisms have revealed that the translation status of mRNAs responds dramatically to different environmental stresses and genetic lesions in the translation apparatus. To identify additional features of the global landscape of translational control, we used microarray analysis of polysomal as well as non-polysomal mRNAs to examine the defects in translation in a poly(A) binding protein mutant, pab2 pab8, as well as in a mutant of a large ribosomal subunit protein, rpl24b/shortvalve1.Results
The mutation of RPL24B stimulated the ribosome occupancy of mRNAs for nuclear encoded ribosomal proteins. Detailed analysis yielded new insights into the translational regulon containing the ribosomal protein mRNAs. First, the ribosome occupancy defects in the rpl24b mutant partially overlapped with those in a previously analyzed initiation factor mutant, eif3h. Second, a group of mRNAs with incomplete coding sequences appeared to be uncoupled from the regulon, since their dependence on RPL24B differed from regular mRNAs. Third, different sister paralogs of the ribosomal proteins differed in their translation state in the wild-type. Some sister paralogs also differed in their response to the rpl24b mutation. In contrast to rpl24b, the pab2 pab8 mutant revealed few gene specific translational defects, but a group of seed storage protein mRNAs were stimulated in their ribosome occupancy. In the course of this work, while optimizing the statistical analysis of ribosome occupancy data, we collected 12 biological replicates of translation states from wild-type seedlings. We defined 20% of mRNAs as having a high variance in their translation state. Many of these mRNAs were functionally associated with responses to the environment, suggesting that subtle variation in the environmental conditions is sensed by plants and transduced to affect the translational efficiency of hundreds of mRNAs.Conclusions
These data represent the first genome-wide analysis of translation in a eukaryote defective in the large ribosomal subunit. RPL24 and eIF3h play similar but non-identical roles in eukaryotic translation. The data also shed light on the fine structure of the regulon of ribosomal protein mRNAs.939.
940.
Yong Wang Jae Young Choi Jong Yul Roh Xue Ying Tao Qin Liu Joo Hyun Lee Jae Su Kim Woo Jin Kim Yeon Ho Je 《Entomological Research》2013,43(1):63-69
Baculovirus chitinase gene (chiA) is a late gene essential for liquefying the host insect at a late stage of infection for its hydrolyzing chitin function. In a previous report, baculovirus ChiA has been shown to offer many interesting new opportunities for pest control. Recently, a putative chiA gene was identified in the Korean isolate of the Spodoptera litura nucleopolyhedorvirus (SpliMNPV‐K1) genome. The open reading frame (ORF) contains 1692 nucelotides and encodes a protein of 563 amino acids with a predicted molecular weight of about 62.6 kDa. To study the insecticidal activity of ChiA from SpliMNPV‐K1, we constructed a recombinant AcMNPV, Ap‐SlChiA, which is designed to express the ChiA under the control of a polyhedrin promoter. Western blot analysis indicated that ChiA was successfully expressed by this recombinant virus. Chitinase assay revealed that the chitobiosidase and endochitinase activity of the recombinant virus was 2.5‐ and 3.9‐flods higher than those of wild‐type AcMNPV, respectively. In addition, the recombinant virus showed higher evident insecticidal activity against 3rd instar larvae of Spodotera exigua than that of the AcMNPV. These results suggest that the chiA gene from SpliMNPV‐K1 could be successfully applied to improve pathogenicity of baculoviruses. 相似文献