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61.
BRANT C. FAIRCLOTH GWEN P. KELLER C. JOSEPH NAIRN WILLIAM E. PALMER JOHN P. CARROLL PATRICIA ADAIR GOWATY 《Molecular ecology resources》2006,6(3):646-649
We describe primers and polymerase chain reaction (PCR) conditions to amplify 18 tetranucleotide microsatellite DNA loci in eastern bluebirds (Sialia sialis). The primers were tested using individuals from two study sites in Georgia and South Carolina. Among individuals from the Georgia population (n = 23), the primer pairs developed in this study yielded an average of 6.6 alleles per locus (range 2–12), an average observed heterozygosity of 0.56 (range 0.24–0.96) and an average polymorphic information content of 0.65 (range 0.3–0.86). Among individuals from the South Carolina population (n = 19), the primer pairs yielded an average of 5.8 alleles per locus (range 2–9), an average observed heterozygosity of 0.56 (range 0.05–0.86) and an average polymorphic information content of 0.63 (range 0.29–0.83). 相似文献
62.
The Australasian‐Pacific and South‐East Asian species of the new orb‐weaving spider genus Plebs with Plebs eburnus (Keyserling, 1886) as type species are revised. Following this study, Plebs includes a total of 22 species of which seven are here described new. Seven species are found in Australia, two in the Pacific region (New Caledonia, Vanuatu), and two in South‐East Asia (Papua New Guinea, The Philippines). Eleven Asian species are transferred to the new genus. Plebs represent comparatively small orb‐weaving spiders of c. 1.2–15.0 mm body length with a slightly elongated abdomen and humeral (shoulder) humps. Males of most species have two to three stout setae on the ventral side of their fourth coxae. Male pedipalps are characterized by the presence of a single macroseta on the patella, the presence of a paramedian apophysis as basal extension of the conductor, and an apical tegular protrusion. The female epigyne has a scape that is generally much longer than wide. It does not have a terminal pocket and is frequently broken off in a number of species. A phylogenetic analysis of 15 species of Plebs (those for which both sexes are known), 13 Australian/Pacific orb‐weaving spider species representing the most commonly collected clades with paramedian apophysis, three species of Nearctic Eriophora Simon, 1864, and Araneus diadematus Clerck, 1758, as outgroup, identified a single synapomorphy of Plebs based on 35 morphological and three behavioural characters: a distinct, inverted U‐shaped light pattern on the ventral side of the abdomen with two additional white spots anterolateral to the spinnerets. This analysis recovered a monophyletic clade of all Asian Plebs, suggesting a single colonization event of the genus that putatively originated in Australia. Most Plebs species appear to be active during the day. They build a regular orb‐web with vertical stabilimentum in grass and low shrubs. © 2012 The Linnean Society of London, Zoological Journal of the Linnean Society, 2012, 166 , 279–341. 相似文献
63.
JOSEPH P. BOTTING LUCY A. MUIR SHUHAI XIAO XIANGFENG LI JIH‐PAI LIN 《Lethaia: An International Journal of Palaeontology and Stratigraphy》2012,45(4):463-475
Botting, J.P., Muir, L.A., Xiao, S., Li, X. & Lin, J.‐P. 2012: Evidence for spicule homology in calcareous and siliceous sponges: biminerallic spicules in Lenica sp. from the Early Cambrian of South China. Lethaia, Vol. 45, pp. 463–475. The relationships of the extant sponge classes, and the nature of the last common ancestor of all sponges, are currently unclear. Early sponges preserved in the fossil record differ greatly from extant taxa, and therefore information from the fossil record is critical for testing hypotheses of sponge phylogenetic relationships that are based on modern taxa. New specimens of the enigmatic sponge Lenica sp., from the Early Cambrian Hetang Biota of South China, exhibit an unusual spicule structure. Each spicule consists of a siliceous core with an axial canal, an organic outer layer and a middle layer interpreted to have been originally calcium carbonate. This finding confirms previous work suggesting the existence of biminerallic spicules in early sponges. Combined with data from other early sponges, the new findings imply that the two fundamental spicule structures of modern sponges were derived from a compound, biminerallic precursor. Spicules are therefore homologous structures in Calcarea and Silicea, and if sponges are paraphyletic with respect to Eumetazoa, then spicules may also have been a primitive feature of Metazoa. □Calcarea, Early Cambrian, Hetang Biota, phylogeny, Silicea, taphonomy. 相似文献
64.
1. Employing field-deployed mesocosms, we examined the effects of 12-h pulse and 20-day press (continuous) exposures of the common agricultural insecticide, imidacloprid, on nymph abundance, emergence patterns and adult body size of Epeorus spp. (Heptageniidae) and Baetis spp. (Baetidae).
2. In press exposures, reduced nymph density was driven by reduced survivorship; in pulse exposures, reduced nymph density may reflect increased emergence because of stress.
3. Once exposed to imidacloprid, Epeorus and Baetis mayflies developed less and emerged smaller than their control counterparts. Concentrations as low as 0.1 μ g L−1 (12-h pulse) reduced head length in Baetis and thorax length in Epeorus .
4. In all of the Baetis and Epeorus examined, effects were only found in males. Male survivorship and body size can affect population dynamics. Sublethal doses of this widely applied agricultural insecticide have the potential to reduce reproductive success of mayfly populations. 相似文献
2. In press exposures, reduced nymph density was driven by reduced survivorship; in pulse exposures, reduced nymph density may reflect increased emergence because of stress.
3. Once exposed to imidacloprid, Epeorus and Baetis mayflies developed less and emerged smaller than their control counterparts. Concentrations as low as 0.1 μ g L
4. In all of the Baetis and Epeorus examined, effects were only found in males. Male survivorship and body size can affect population dynamics. Sublethal doses of this widely applied agricultural insecticide have the potential to reduce reproductive success of mayfly populations. 相似文献
65.
Detection of Epstein-Barr Viral Genome in Nonproductive Cells 总被引:63,自引:0,他引:63
Hybridization experiments have shown that EB viral DNA comprises between 0.06 and 1.6% of the total DNA of Burkitt lymphoma cells and established leucocyte cell lines. 相似文献
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69.
P. E. VAUDAUX N. E. WILLIAMS J. FRANKEL C. VAUDAUX 《The Journal of eukaryotic microbiology》1977,24(3):453-458
SYNOPSIS The high molecular weight proteins found in isolated pellicles of Tetrahymena have been compared in several individual strains within the genus using SDS polyacrylamide gel electrophoresis. Three variants of the B-protein of epiplasm (MW 174,000; 155,000; 145,000) and 2 of the C-protein (MW 140,000; 122,000) were found among the strains examined. No variation was observed in the major kinetodesmal fiber protein (MW 250,000). The variation found between strains in the proteins of a structure which is (as far as we know) the same in all strains indicates a disjunction between evolutionary change at the 2 levels of organization. The taxonomic implications of the observed variation in structural proteins in Tetrahymena are discussed. 相似文献
70.
ABSTRACT. . An improved model for the repellent effect of pyrethroid insecticides on insects was developed using small colonies of honey bees, Apis mellifera L., in flight cages. Conditioning to scented feeders allowed the separation of foraging bees from a single colony into paired treatment and control groups. The repellent response was characterized as a sublethal toxic effect resulting in transitory inhibition of activity. Permethrin and cypermethrin were shown to be contact repellents to honey bees; exposure was primarily to the tarsi and abdominal venter. Repellency was fully reversible within 24 h. No permanent effects on either memory function or foraging efficiency were observed following acute exposure. 相似文献