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51.
Dioon edule Lindl. (Zamiaceae) is a cycad endemic to Mexico, that occurs as one species D. edule and the geographical variety D. edule var. angustifolium (Miq.) Miq. Dioon edule has a north to south distribution in eastern Mexico. In this study, we analysed 14 allozymic loci in eight populations of D. edule from its total distribution range by sampling all known populations. Patterns of diversity and genetic variability, within and among populations, were obtained. The mean number of alleles per locus ( A ) was 1.44 and the percentage of polymorphic loci was relatively high ( P  = 54.78). The mean observed ( H O ) and expected heterozygosity ( H E ) were 0.27 and 0.24, respectively. F -statistics revealed an excess of heterozygous genotypes, locally and globally ( F  = −0.17 and f  = −0.27, respectively). The genetic variation explained by differences among populations was only 7.5%. We also detected a negative relationship between genetic diversity and latitude. On average, the gene flow between population pairs was relatively high ( Nm  = 2.98); furthermore, gene flow between population pairs was significantly correlated with geographical distances ( r  = −0.38, P  = 0.025). Therefore, patterns of genetic diversity in D. edule appear to be associated with the post-Pleistocene spread of the species, from its southerly (origin) to its northerly range (derived populations, including its central distribution). The biogeographical and evolutionary aspects of the results of this study are discussed. We recognize Dioon angustifolium Miq. for the northernmost disjunct populations.  © 2003 The Linnean Society of London, Biological Journal of the Linnean Society , 2003, 80 , 457–467  相似文献   
52.
THE concentrations of tryptophan normally present in the mammalian brain are below the Michaelis constant (Km) of tryptophan hydroxylase1,2, suggesting that the rate of serotonin synthesis depends more on the concentration of brain tryptophan than on the amount of enzyme. We wish to report that various treatments which have been shown to increase brain serotonin synthesis also increase the concentration of tryptophan in brain. Conversely, p-chlorophenylalanine (PCPA), which inhibits serotonin synthesis3, decreases tryptophan in brain.  相似文献   
53.
Ultrastructural studies (SEM and TEM) were performed on cotyledonsof pineapple guava ( Feijoa sellowiana Berg, Myrtaceae) inducedto form embryos on medium containing 1.0 mg l-1(4.5µM2,4-D) and 0.3M sucrose. At the time of culture, the cells werefilled with protein and lipid bodies. Microbodies and poorlydifferentiated organelles could also be seen. In contrast togerminating cotyledons, where lipid and protein reserves werequickly metabolized, cells of the embryogenically induced cotyledonsshowed evidence of reserve consumption only after 5 d of culture.Subepidermal cells of the upper cotyledonary surface underwentseveral divisions giving rise to a meristematic layer of severalcells thickness from which somatic embryos developed. Embryoscould also be formed directly by successive divisions of epidermalcells. Cells involved in somatic embryo formation containeda large nucleus with a conspicuous nucleolus and dense cytoplasmwhere numerous ribosomes, mitochondria, plastids with starchand short profiles of rough endoplasmic reticulum were present.Plasmodesmata were present both in cell walls of the meristematiccells and in few celled embryos whereas in degenerating embryosor in more advanced stages of somatic embryo development noplasmodesmata could be found. Although oil bodies were not observedin the meristematic cells they were identified in very youngembryos, being the first reserve compounds to appear. Cellsnot involved in somatic embryo differentiation were characterizedby the presence of several microbodies containing a crystalloidinclusion and elongated mitochondria. Feijoa sellowiana ; pineapple guava; somatic embryogenesis; ultrastructural studies  相似文献   
54.
ABSTRACT. Serine is an important amino acid that is utilized in the biosyntheses of proteins and lipids. It is directly incorporated into the head group of phosphatidylserine, which in turn can be converted to other phospholipids. Also, it is required for the formation of long chain bases, precursors of sphingolipids. Uptake and incorporation of radiolabeled serine into both lipids and acid-precipitable material were demonstrated in Pneumocystis carinii carinii organism preparations freshly isolated from infected rat lungs. Radioactivity in proteins was about double that observed in lipids. Liquid scintillation spectrometry of metabolically radiolabeled lipids separated by thin-layer chromatography showed 53% of the total radioactivity were in phosphatidylserine, 12% in phosphatidylethanolamine, 24% in ceramides, and 11% in long chain bases and other compounds. Four long chain bases were detected by thin-layer chromatography in hydrolyzed P. carinii ceramides metabolically labeled with radioactive serine. Phytosphingosine and dihydrosphingosine were tentatively identified by their migrations on thin-layer plates. Radiolabeled ethanolamine was incorporated into P. carinii phosphatidylethanolamine, but relatively low incorporation of radiolabeled choline into phosphatidylcholine occurred. The observations made in this study indicated that P. carinii has the biosynthetic capacity to metabolize phospholipid head groups and to de novo synthesize sphingolipids. L-Cycloserine and β-CI-D-alanine, inhibitors of long chain base synthesis, reduced the incorporation of serine into P. carinii long chain bases and ceramides, which supported the conclusion that the pathogen synthesizes sphingolipids.  相似文献   
55.
During the highest spring tides the intertidal sediment flats of estuaries are fully inundated at high water, and waders have no choice but to move to supratidal roosts, e.g. on open farmland, saltpans or beaches. However, in many estuaries during the lowest neap or intermediate tides there are sectors of upper intertidal sediment flats that remain exposed even at the peak of high water, and so waders have the choice of roosting either there or in supratidal sites. In the Tagus Estuary, Portugal, as elsewhere, waders use both types of roosts during high water. Our main objective was to understand what makes waders opt for one of these two types of available roosts. We monitored wader use of saltpans and intertidal roosts from spring to neap tides, and measured foraging and alarm behaviour, prey availability and disturbance by predators. Most of the wader species studied chose intertidal (mudflat) roosts whenever these were available, and only roosted in saltpans during the peak of spring tides. We hypothesized that this preference was explained either by an attempt (i) to continue feeding into the high water period, or (ii) to minimize predation risk. Extending feeding time into the high water period did not seem to be very relevant for roost choice because both prey availability and foraging activity were low in both types of roosts. However, predator disturbance was several times higher in the saltpans than in the intertidal roosts, suggesting that this factor may be the determinant in the choice of roost type.  相似文献   
56.
57.
ABSTRACT.
  • 1 The butterfly Sandia xami (Reakirt) has a very clumped yearly pattern of oviposition on its food plant Echeveria gibbiflora D.C., and the mean number of eggs per plant is always very low.
  • 2 Three features of host plants are highly associated with the probability of being oviposited upon: height of plant; degree of conspicuousness; and degree of isolation from conspecific plants.
  • 3 The relative importance of these factors as predictors of the probability of oviposition change according to the time scale considered: height and conspicuousness are more important over short (weekly) time intervals whilst isolation takes precedence over longer (yearly) periods.
  • 4 It is hypothesized that the clumped pattern of oviposition is responsible for the low numbers of the butterflies relative to their very abundant food plant.
  相似文献   
58.
It has been demonstrated that mosquito larvae crude extract has an inhibiting effect on the mitotic rate of several mouse cell populations. The sampling period was 16–24h after treatment, when mitotic peak normally occurs. The present paper reports the effect of mosquito larvae crude extract on the proliferation of hepatocytes, renocytes, Lieberkhün crypt enterocytes, and sialocytes. In this case, the sampling period covered the dark phase of the day, during the first 12h after treatment. Colchicine-arrested metaphases were controlled at 20/04, 00/08 and 04/12 (Time of Day/Time Post Injection). The mitotic rate was significantly increased in hepatocytes and renocytes and inhibited in duodenum enterocytes. In view of the time chosen to administer the treatments and the time elapsed until sampling, we conclude a probable effect of the extract at the G2-M point of the cell cycle.  相似文献   
59.
60.
The effect of vesicles of purified egg yolk phosphatidylcholine on the fertilizing capacity and acrosome breakdown of amphibian spermatozoa was studied. When Bufo arenarum spermatozoa were incubated with either small unilamellar vesicles (prepared by sonication) or with large unilamellar vesicles (prepared by reverse-phase evaporation) a decrease in the fertilizing capacity of spermatozoa was found. At the same phosphatidylcholine concentration, large unilamellar vesicles were more inhibitory than small unilamellar vesicles. The inhibition was dependent upon the phospholipid concentration and the length of the incubation period. Small unilamellar vesicles did not modify the time course of acrosome breakdown in Leptodactylus chaquensis , while large unilamellar vesicles markedly accelerated the rate of acrosome breakdown. In both biossays, the charge of the vesicles (made either positive or negative by the addition of 5% stearylamine or 5% phosphatidic acid) did not influence their biological effect. Multilamellar vesicles did not alter the fertilizing capacity nor the acrosome breakdown. We conclude that the size and the structure of the vesicles are important parameters in determining the inhibitory capacity of phosphatidyl choline on amphibian fertilization.  相似文献   
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