全文获取类型
收费全文 | 153篇 |
免费 | 3篇 |
出版年
2019年 | 1篇 |
2018年 | 1篇 |
2017年 | 3篇 |
2015年 | 3篇 |
2013年 | 8篇 |
2012年 | 6篇 |
2011年 | 7篇 |
2010年 | 10篇 |
2009年 | 12篇 |
2008年 | 8篇 |
2007年 | 11篇 |
2006年 | 4篇 |
2005年 | 8篇 |
2004年 | 3篇 |
2003年 | 5篇 |
2002年 | 1篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1999年 | 3篇 |
1998年 | 4篇 |
1997年 | 4篇 |
1996年 | 5篇 |
1995年 | 2篇 |
1994年 | 3篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 4篇 |
1990年 | 1篇 |
1989年 | 5篇 |
1987年 | 2篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 4篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1976年 | 1篇 |
1975年 | 1篇 |
1973年 | 2篇 |
1972年 | 4篇 |
1971年 | 2篇 |
1966年 | 1篇 |
排序方式: 共有156条查询结果,搜索用时 538 毫秒
61.
62.
63.
JENNIFER G. PARSONS DAVID BLAIR JON LULY SIMON K. A. ROBSON 《The Journal of wildlife management》2009,73(4):526-529
ABSTRACT Bat collisions are a threat to commercial and military aircraft in Australia. We examined bat strike records from Australia during 1996–2006 and found that risk of impact from bats is increasing, is greatest in tropical versus temperate regions, and is more likely during early evening and while an aircraft is landing rather than departing. Temporal patterns of bat strikes differ from those of birds, highlighting the need to employ taxon-specific management strategies to minimize animal impacts on the aviation industry. The use of genetics for identification of strike remains and the implementation of nocturnal survey techniques by wildlife managers at airports will contribute to the mitigation of bat strikes. 相似文献
64.
Peripheral alpha1,3-fucosylation of glycans occurs by the action of either
one of five different alpha1,3-fucosyltransferases (Fuc-Ts) cloned to date.
Fuc-TVI is one of the alpha1,3-fucosyltransferases which is capable to
synthesize selectin ligands. The major alpha1, 3- fucosyltransferase
activity in human plasma is encoded by the gene for fucosyltransferase VI,
which presumably originates from liver cells. While the sequence,
chromosomal localization, and kinetic properties of Fuc-TVI are known,
immunocytochemical localization and trafficking studies have been
impossible because of the lack of specific antibodies. Here we report on
the development and characterization of a peptide-specific polyclonal
antiserum monospecific to Fuc-TVI and an antiserum to purified soluble
recombinant Fuc-TVI crossreactive with Fuc-TIII and Fuc-TV. Both antisera
were applied for immunodetection in stably transfected CHO cells expressing
the full-length form of this enzyme (CHO clone 61/11). Fuc-TVI was found to
be a resident protein of the Golgi apparatus. In addition, more than 30% of
cell-associated and released enzyme activity was found in the medium.
Maturation and release of Fuc-TVI was analyzed in metabolically labeled CHO
61/11 cells followed by immunoprecipitation. Fuc-TVI occurred in two forms
of 47 kDa and 43 kDa bands, while the secreted form was detected as a 43
kDa. These two different intracellular forms arose by posttranslational
modification, as shown by pulse-chase experiments. Fuc-TVI was released to
the supernatant by proteolytic cleavage as a partially endo-H resistant
glycoform.
相似文献
65.
JOHN D. C. LINNELL RONNY AANES JON E. SWENSON JOHN ODDEN MARTIN E. SMITH 《Biodiversity and Conservation》1997,6(9):1245-1257
Translocation of individual carnivores has been a standard management tool for decades in North America and southern Africa
in response to livestock depredation and other conflict behaviours. As carnivore populations across Europe begin to increase
it is expected that management problems will also increase. Before translocation becomes established as a management tool
in Europe its success needs to be reviewed. In general, there has been very little follow-up of translocated animals. Almost
no data exist on the subsequent levels of damage after translocation. Large carnivores have shown a consistent ability to
return to the site of capture over distances of up to 400 km. Even those individuals that do not succeed in returning home
roam over very large distances, best measured in units of hundreds of kilometres. Very few individuals remain at the release
sites. Survival of translocated animals has occasionally been shown to be poor, often as a result of the large movements.
In general, there needs to be a large area (hundreds or thousands of square kilometres) without conflict potential where the
individuals can be released for the strategy to work. When such areas are not available, management efforts should concentrate
on reducing conflict potential, or, where this is not practical, lethal control.
This revised version was published online in November 2006 with corrections to the Cover Date. 相似文献
66.
JON R. BRIDLE ANN-KATRINE GARN KATE A. MONK ROGER K. BUTLIN 《Biological journal of the Linnean Society. Linnean Society of London》2001,72(3):373-390
We investigate colour pattern and morphological variation in Chitaura grasshoppers on the Indonesian island of Sulawesi, and examine the relationship between divergence in these two sets of characters and population history, as reflected by variation in mitochondrial DNA. Analysis of colour pattern variation identifies a total of 21 distinct Operational Taxonomic Units in Sulawesi, the majority of which have parapatric distributions. Patterns of phenotypic variation at two contact zones in north Sulawesi suggest genetic independence between three of these colour forms, indicating that speciation has occurred. Despite this, colour pattern divergence is only coincident with morphological differentiation at one of these contact zones. In addition, neither type of phenotypic divergence is associated with geographical structuring in mitochondrial DNA, suggesting that historical isolation has had little influence on evolutionary diversification. Instead, divergence in colour pattern and morphology appears to have occurred rapidly or under conditions of continued gene flow, possibly in response to spatially variable natural selection. This result has implications for the identification of conservation units based solely on molecular markers. 相似文献
67.
MIRIAM TEUSCHER MARTIN BRÄNDLE VERENA TRAXEL ROLAND BRANDL 《Ecological Entomology》2009,34(6):718-724
- 1 The size–grain hypothesis ( Kaspari & Weiser, 1999 ) states that (1) as organisms decrease in size, they perceive their environment as being more rugose; (2) long legs allow organisms to step over obstacles but hinder them from entering small gaps; and (3) as the size of an organism decreases, the benefits of long legs begin to be outweighed by the costs of construction. Natural selection should therefore favour proportionally longer legs in larger organisms, thereby leading to a positive allometry between leg and body length (scaling exponent b > 1).
- 2 Here we compare the scaling exponent of leg‐to‐body length relationships among insects that walk, walk and fly, and predominantly fly. We measured the lengths of the hind tibia, hind femur, and body length of each species.
- 3 The taxa varied considerably in the scaling exponent b. In seven out of ten groups (Formicidae, Isoptera, Carabidae, Pentatomidae, Apidae, Lepidoptera, Odonata adult), b was significantly greater than one. However, there was no gradual decrease in b from walking to walking/flying to flying insects.
- 4 The results of the present study provide no support for the size–grain hypothesis. We propose that leg length is not only affected by the rugosity of the environment, but also by (1) functional adaptations, (2) phylogeny, (3) lifestyle, (4) the type of insect development (hemimetabolism or holometabolism), and (5) constraints of gas exchange.
68.
Hybridization and polyploidy as drivers of continuing evolution and speciation in Sorbus 总被引:1,自引:0,他引:1
ASHLEY ROBERTSON TIMOTHY C. G. RICH ALEXANDRA M. ALLEN LIBBY HOUSTON CAT ROBERTS JON R. BRIDLE STEPHEN A. HARRIS SIMON J. HISCOCK 《Molecular ecology》2010,19(8):1675-1690
Interspecific hybridization and polyploidy are pivotal processes in plant evolution and speciation. The fate of new hybrid and polyploid taxa is determined by their ability to reproduce either sexually or asexually. Hybrids and allopolyploids with odd chromosome numbers are frequently sterile but some establish themselves through asexual reproduction (vegetative or apomixis). This allows novel genotypes to become established by isolating them from gene flow and leads to complex patterns of variation. The genus Sorbus is a good example of taxonomic complexity arising from the combined effects of hybridization, polyploidy and apomixis. The Avon Gorge in South‐west Britain contains the greatest diversity of Sorbus in Europe, with three endemic species and four putative endemic novel hybrids among its 15 native Sorbus taxa. We used a combination of nuclear microsatellite and chloroplast DNA markers to investigate the evolutionary relationships among these Sorbus taxa within the Avon Gorge. We confirm the genetic identity of putative novel taxa and show that hybridization involving sexual diploid species, primarily S. aria and S. torminalis and polyploid facultative apomictic species from subgenus Aria, has been responsible for generating this biodiversity. Importantly our data show that this creative evolutionary process is ongoing within the Avon Gorge. Conservation strategies for the rare endemic Sorbus taxa should therefore consider all Sorbus taxa within the Gorge and must strive to preserve this evolutionary process rather than simply the individual rare taxa that it produces. 相似文献
69.
ABSTRACT Distress calls are signals effective over a long distance. They are well known to evoke interspecific reactions. We suggest that the interspecificity phenomenon results from the use of similar laws of decoding by the species concerned. These laws must take into account the transmission channel which always has a great influence on long-range communication. We tested our hypothesis by broadcasting simplified synthetic calls to two species of birds: the herring gull and the starling. The various calls differed in terms of frequency modulation (FM). Two main conclusions emerged from this series of tests: 1. The parameters used for recognition are not sophisticated: a simple slope applied to a carrier frequency that corresponds to the acoustic shape of a distress call is sufficient to confer a distress meaning to the signal. The basic rules are the same for the gull and the starling, with differences only in the acceptance level of the species. 2. The system of recognition is based upon parameters not altered by the environment: the birds make use of the slow frequency modulations (FMI). In contrast, the fast frequency sweeps (FMII) which are modified during propagation do not seem to be utilised. The use of these characteristics of distress calls for recognition allows interspecificity and maximum efficiency for propagation over long distances.
RESUME
Une particularité des cris de détresse est leur action interspécifique. Nous suggérons que cette interspécificité résulte de l'emploi d'une loi de décodage qui est commune à plusieures espèces. Cette loi doit tenir compte des propriétés du canal car ce dernier a toujours une grande influence sur les signaux de longue portée acoustique, tels que les cris de détresse. Pour tester cette hypothèse, nous avons fait appel à deux espèces: le Goéland argenté (Larus argentatus) et l'Etourneau (Sturnus vulgaris). Les signaux que nous avons diffusés étaient obtenus par synthèse. Ils étaient plus simples que les signaux naturels et différaient par la modulation de fréquence (FM). Deux résultats principaux émergent de ces séries d'expériences: 1. Le procédé de décodage n'est pas sophistiqué: une simple pente appliquée à la porteuse est nécessaire et il suffit de mimer, meme approximativement, la pente rencontrée dans les signaux naturels, Cette règle fondamentale est commune au Goéland et à l'Etourneau. Les petites différences que l'on peut néanmoins observer sont relatives aux seuils de décodage. 2. Le décodage repose sur des paramètres qui ne sont pas altérés par le canal: l'oiseau utilise la modulation lente de fréquence. Par contre les excursions rapides de fréquence semblent n'étre d'aucune utilité lors du décodage. En conclusion, l'universalité des paramètres impliqués et de la loi de décodage est responsable de l'interspécificité des signaux de détresse. Les simplifications liées à cette universalité résultent des contraintes imposées par le canal. 相似文献
70.
Methicillin-resistant Staphylococcus aureus (MRSA) poses a serious problem in dairy animals suffering from mastitis. In the present study, the distribution of mastitic
MRSA and antibiotic resistance was studied in 107 strains of S. aureus isolated from milk samples from 195 infected udders. The characterizations pathogenic factors (adhesin and toxin genes) and
antibiotic susceptibility of isolates were carried out using gene amplification and disc diffusion assays, respectively. A
high prevalence of MRSA was observed in the tested isolates (13.1%). The isolates were also highly resistant to antibiotics,
i.e. 36.4% were resistant to streptomycin, 33.6% to oxytetracycline, 29.9% to gentamicin and 26.2% each to chloramphenicol,
pristinomycin and ciprofloxacin. A significant variation in the expression of pathogenic factors (Ig, coa and clf) was observed in these isolates. The overall distribution of adhesin genes ebp, fib, bbp, fnbB, cap5, cap8, map and cna in the isolates was found to be 69.1, 67.2, 6.5, 20.5, 60.7, 26.1, 81.3 and 8.4%, respectively. The presence of fib, fnbB, bbp and map genes was considerably greater in MRSA than in methicillin-susceptible S. aureus (MSSA) isolates. The proportions of toxin genes, namely, hlb, seb, sec, sed, seg and sei, in the isolates were found to be 94.3, 0.9, 8.4, 0.9, 10.2 and 49.5%, respectively. The proportions of agr genes I, II, III and IV were found to be 39.2, 27.1, 21.5 and 12.1%, respectively. A few isolates showed similar antibiotic-resistance
patterns, which could be due to identical strains or the dissemination of the same strains among animals. These findings can
be utilized in mastitis treatment programmes and antimicrobials strategies in organized herds. 相似文献