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51.
52.
ABSTRACT. Ameba to flagellate transformation in Naegleria fowleri (Lovell strain) was affected by growth temperature, phase of growth, strain of ameba, culture agitation, enflagellation temperature, enflagellation diluent, and cell concentration. Amebae transformed best when they were grown without agitation and enflagellated with agitation. Regardless of growth temperature (23°, 30°, 37°, and 42°C were tested), amebae transformed best at 37°C. Enflagellation was greatest for cells harvested between 24 h (mid-exponential) and 84 h (late stationary) of growth. 相似文献
53.
LIGHT ABSORPTION BY PLANTS AND ITS IMPLICATIONS FOR PHOTOSYNTHESIS 总被引:10,自引:0,他引:10
BRUCE A. OSBORNE JOHN A. RAVEN 《Biological reviews of the Cambridge Philosophical Society》1986,61(1):1-60
The preceding account has attempted to examine the interactions between light absorption and photosynthesis, with reference to both unicellular and multicellular terrestrial and aquatic plants. There are, however, some notable plant groups to which no direct reference has been made, e.g. mosses, liverworts and lichens. Although many have similar optical properties to terrestrial vascular plants (Gates, 1980) and apparently similar photosynthetic responses (see Green & Snelgar, 1982; Kershaw, 1984) they may possess subtle, as yet unknown differences. For instance, the lichen thallus has a high surface reflectance although the transmittance is virtually zero (Gates, 1980; Osborne, unpublished results). It is envisaged, however, that differences in optical properties between species will reflect differences in degree not kind. Although not all variation in photosynthesis is due to differences in light absorption a number of accounts suggest that this is a contributing factor. Variations in leaf absorptance have been found to account for most of the variation in leaf photosynthesis at low Jis (see Ehleringer & Björkman, 1978a; Osborne & Garrett, 1983). There is, however, little direct experimental evidence on light absorption and photosynthesis in either microalgal species or aquatic macrophytes. We also do not know over what range of incident photon flux densities photosynthesis is determined largely by changes in light absorption. Plants growing under natural conditions also experience large diurnal and seasonal fluctuations in Ji, unlike species grown under laboratory conditions. The occurrence of transitory peaks in Ji tends to overshadow the fact that the average Ji is often lower than the J1 required to saturate photosynthesis, i.e. 1500–2000 μmol m-2 s-1, depending on the growth treatment. Using the data of Monteith (1977) and I W m2= 5 μmol m-2 s-1, and with photosynthetically active radiation 50% of total solar radiation, the daily mean value for Britain is approximately 450 μmol m-2 s-1, with a maximum in June of 1000μmol m-2 s-1 and a minimum during the winter of 75 μmol m-2 s-1. Such values could be even lower on shaded understory leaves and considerably lower for aquatic species. Based on average values of net photosynthesis for a terrestrial plant leaf, light saturation would only be expected in June while for most of the year the average values would lie largely on the light-limited portion of the photosynthesis light response curve. Although the daily average values in tropical climates may be higher during the winter months, they are remarkably similar throughout the world for the respective summers in the northern and southern hemispheres, because the increased daylength at high latitudes compensates for the lower Jis. The expected lower dark respiration rates during the winter may also partially offset the effects of a lower light level. There is therefore a trade-off between high Jis for a short period of time against a lower Ji for a longer period of time. We might expect different photosynthetic responses to these two very different conditions. Importantly, a low Ji with a long daylength may enable a plant to photosynthesize at or near its maximum photon efficiency for most of the day. Although the response of the plant to fluctuations in Ji is complicated because it is affected by the previous environmental conditions, this may indicate that light absorption has a much greater significance under natural conditions, particularly for perennial species. The bias in many laboratories towards research on terrestrial vascular plants also tends to ignore the fact that a number of multicellular and unicellular aquatic species survive in very low light environments. Furthermore, the direct extrapolation of photosynthetic responses from measurements on single leaves to those of whole plants is clearly erroneous. Although this is obvious, many physiological ecologists have attributed all manner of things to the photosynthetic responses of ‘primary’ leaves. Most researchers have ignored problems associated with composite plant tissues and internal light gradients. Clearly caution is required in interpreting the photosynthesis light-response curve of multicellular tissues based on biochemical features alone. Also, the importance of cell structure on light absorption and photosynthesis has generally been ignored and attributed solely to the effects of structural features on CO2 diffusion. In doing so the work of two or three generations of plant physiologists has been ignored. Haberlandt (1914) at the turn of the century probably first implicated the role of cell structure in leaf optics, and Heath (1970) stressed that in order to completely understand the role of light in photosynthesis we need to know the flux incident on the chloroplast itself. Even this suggestion may need modification because of the capacity of the internal chloroplast membranes for scattering light. It is worth emphasizing the importance of light gradients within tissues and their role in regulating photosynthesis, particularly at light saturation. Measurements of light gradients are fraught with problems because of experimental difficulties and the majority (few) are based on reflectance and transmittance measurements. Seyfried & Fukshansky (1983) have shown that light incident on the lower surface of a Cucurbita cotyledon produced a larger light gradient than light incident from above, indicating the importance of the spatial arrangement of the tissues with respect to the light source. Also, light incident on the lower surface of leaves of Picea sitchensis was less ‘effective’ in photosynthesis than light from above (Leverenz & Jarvis, 1979). Clearly, two tissues could have the same gross absorptance but different photosynthetic rates because of differences in the internal light environment. Fisher & Fisher (1983) have recently found asymmetries in the light distribution within leaves, which they related to asymmetries in photosynthetic products due to differences in solar elevation. Such modifications in light distribution could be important for a number of solar-tracking species. Changes in light absorption are brought about by a whole gamut of physiological, morphological and behavioural responses which serve to optimize the amount of light absorbed. Perhaps the simplest way of regulating the amount of light absorbed is by restricting growth either to particular times of the year or to conditions when the light climate is favourable. We are still largely ignorant of many details of these modifications. In particular, differences in tissue structure such as the size and number of vacuoles or the effects of organelles on the scattering component of the internal light environment of photosynthetic tissues are not understood. A better understanding of the interaction of light with plants in aquatic systems is also required. It is unfortunate that light-absorptance measurements are not routinely made in photosynthetic studies, and this is quite clearly a neglected area of study. That these measurements are not made is even more surprising, since techniques have been available for over sixty years (Ulbricht, 1920). Absorptance measurements are of particular importance in the photosynthetic adaptation of microalgae, where only a small proportion of the incident photon flux density is absorbed. For multicellular species more detailed information is required on internal light gradients and their variability. Light-absorptance measurements are also important in any study relating kinetic data on CO2 fixation to in vivo photosynthesis, especially when there are large variations in the morphology and structure of the photosynthetic organ. 相似文献
54.
Species of the genus Paradoxostoma Fischer are an important component of marine and estuarine ostracod faunas throughout the world. This revision of the genus in British waters deals with 17 species of which six are described as new. Each species is diagnosed and illustrated in detail, and comments are made on their ecology and distribution. The specialized mouthparts which characterize the genus are illustrated by means of the scanning electron microscope for the first time. A key to the genus is given. 相似文献
55.
Eight isolates of Naegleria australiensis were obtained from a small lake in Tulsa, Oklahoma. The eight strains were isolated during the hot summer months of July through September, when water temperatures ranged from 27 to 33°C. All eight isolates were pathogenic for mice. The mean time to death for mice was 10 days (range 6–13 days). This pathogenic free-living ameba has not before been reported from the United States or the Western Hemisphere. 相似文献
56.
'Anemotactic' flight paths of tsetse flies in relation to host odour: a preliminary video study in nature of the response to loss of odour 总被引:1,自引:0,他引:1
ABSTRACT. Free-flying, wild Glossina pallidipes Aust. and G. morsitans Westw. were video-recorded in the field in Zimbabwe as they flew out of air permeated with host odour (camera 2.5 m up, looking down at the ground). Analysis of the flight tracks supports the proposal of Bursell (1984) that tsetse flies attracted to an invisible source of host odour respond weakly if at all to wind direction while in flight: on losing contact with the odour the flies made a sharp turn that was uncorrelated with wind direction. The size of the turn varied considerably, with a marked discontinuity in the log-survivorship curve at 120° (a fly which had turned through at least 120° was 5 times as likely to stop the turn as a fly which had turned <120°). Over half the flies made turns of >90° (and <2 m diameter) within the 2×2.5 m field of view of the camera. It is suggested that these turns initially served to arrest the upwind progress of the fly, with the size of the turn determining the degree to which the fly backtracked towards where it last detected odour or continues cross-wind. Mean flight speed was c. 5 ms-1 (min. 2.5, max. probably 7ms-1 ). 相似文献
57.
Envelope development in Trachelomonas lefevrei (Deflandre) begins with the production of short, coarse mucilaginous strands, morphologically similar to the mucilage produced by non-enveloped euglenoids. These initial mucilaginous secretions subsequently become impregnated with manganese (Mn) and/or iron (Fe). Continued mucilage secretion and mineralization results in a mature envelope that is characteristic for the species. When these mature envelopes are treated with oxalic acid to remove the Mn and Fe, the envelopes collapse and are composed only of short, coarse mucilaginous strands similar to those present during early stages of development, prior to their mineralization. Brief treatment with 10 mM EDTA renders dark envelopes colorless, and our EM-EDS analyses show that this corresponds to a loss of Mn from the envelope; however, if Fe is present in the envelope, it is unaffected by the brief treatment. The mucilage present during early stages of envelope development and that remaining after complete demineralization is also morphologically similar to the mucilage in the plug at the anterior end of the envelope. 相似文献
58.
Epeiric sedimentation and sea level: synthetic ecostratigraphy 总被引:2,自引:0,他引:2
JOHN L. CISNE RAYMOND F. GILDNER BRUCE D. RABE 《Lethaia: An International Journal of Palaeontology and Stratigraphy》1984,17(4):267-288
Carbonate strata from the central parts of epicontinental seas are ideal for detailed biostratigraphic study of eustatic sea level change. Using a model for epeiric seas in which carbonate accumulation rate is depth-dependent, we derive synthetic stratigraphies for sea level histories simulating post-glacial transgression and constant and sinusoidally fluctuating ridge volume increase. These sea level histories give distinctively different trends for water depth as a function of stratigraphic position in sections' bathymetric curves. In general, depth is proportional to the rate of sea level rise. Depth-dependent sedimentation leads to a time lag between sea level fluctuation and corresponding depth fluctuation which, as examples show, can approach 106 years for depth fluctuations of only a few meters – a fundamental consideration in reconstructing sea level curves, time-correlating sections by their bathymetric curves, and attempting to relate bathymetric history on continents to mechanisms driving sea level change. Bathymetric curves based on gradient analysis of fossil assemblages (coenocorrelation curves) for Middle Ordovician sections in New York and the American Midwest approximate patterns for sinusoidally increasing sea level. The model's predictions are tested in an ‘artificial experiment’ that takes advantage of differential subsidence between the craton's middle and its edge to make a difference in the bathymetric histories of sections that otherwise record the same sea level history. Depth fluctuations of no more than a few meters over million year spans are potentially useful in time-correlation to within fractions of a cycle's period. The depth-dependence in sedimentation was that, above wave base, net accumulation per year was very roughly three-millionths the water depth. Association of volcanic ash layers with transitory sea level minima on the craton, and with onset of more rapid subduction in the Taconic are - continent collision zone, suggests interrelationship on a million year scale between sea level and large-scale tectonic phenomena. 相似文献
59.
JOHN MOYSE 《Zoological Journal of the Linnean Society》1984,80(1):323-336
Limb movements of restrained stage VI nauplii of Lepas pectinata were studied by cine-photography. Outline drawings were made of successive limb positions in both swimming and grooming activity. The antennae appeared to act as leaky paddles performing both propulsion and food gathering. Free-swimming nauplii averaged 120 limb beats min-1 and a speed of c. 4 mm s-1 . Grooming occurred every 7–20 beats.
It was concluded that lack of streamlining favours filtration at the expense of propulsion. The grooming sequence differs from that of balanid nauplii and is one method of transferring food to the vicinity of the mouth, where sorting and rejection take place prior to ingestion. Fine- and coarse-mesh filters presumably exploit different plankton types. The overall behaviour pattern is well-designed for exploitation of scarce food in the oligotrophic conditions of the ocean-surface habitat. 相似文献
It was concluded that lack of streamlining favours filtration at the expense of propulsion. The grooming sequence differs from that of balanid nauplii and is one method of transferring food to the vicinity of the mouth, where sorting and rejection take place prior to ingestion. Fine- and coarse-mesh filters presumably exploit different plankton types. The overall behaviour pattern is well-designed for exploitation of scarce food in the oligotrophic conditions of the ocean-surface habitat. 相似文献
60.
The effect of changes in the environment on lipid metabolismhas been studied in the brown alga, Fucus serratus L. Lightstimulated the incorporation of radioactivity from /{I4C/}acetateinto oleic and, especially, into linoleic acid. The same effectwas caused by lowering the incubation temperature from 15 °Cto 4 °C. Incubations in the presence of Cd + +, Pb + + orZn+ + had no effect on the total uptake of /{14C/}acetate intothe frond tip samples, but lowered the labelling of total lipidsrelative to aqueous-soluble components. However, pre-exposureof the algae to heavy metal cations caused changes in the uptakeof radioactivity but had less effect on the relative labellingof lipids than incubations in the presence of heavy metal cations.Algae collected from sites where the dissolved levels of heavymetals were elevated, showed a decrease in the relative labellingof lipids from /{14C/}acetate. Concentrations of Cd+ +, Pb++ or Zn+ + at 10 x levels found at the collection site had littleeffect on the pattern of fatty acids made by Fucus serratus. Key words: Lipid metabolism, Fucus serratus L., Environmental changes 相似文献