全文获取类型
收费全文 | 2799篇 |
免费 | 23篇 |
国内免费 | 1篇 |
专业分类
2823篇 |
出版年
2015年 | 15篇 |
2014年 | 16篇 |
2013年 | 62篇 |
2012年 | 50篇 |
2011年 | 71篇 |
2010年 | 115篇 |
2009年 | 101篇 |
2008年 | 108篇 |
2007年 | 140篇 |
2006年 | 75篇 |
2005年 | 86篇 |
2004年 | 51篇 |
2003年 | 29篇 |
2002年 | 19篇 |
2001年 | 26篇 |
2000年 | 25篇 |
1999年 | 38篇 |
1998年 | 58篇 |
1997年 | 86篇 |
1996年 | 68篇 |
1995年 | 72篇 |
1994年 | 47篇 |
1993年 | 59篇 |
1992年 | 48篇 |
1991年 | 62篇 |
1990年 | 57篇 |
1989年 | 60篇 |
1988年 | 52篇 |
1987年 | 66篇 |
1986年 | 46篇 |
1985年 | 54篇 |
1984年 | 50篇 |
1983年 | 59篇 |
1982年 | 37篇 |
1981年 | 36篇 |
1980年 | 43篇 |
1979年 | 53篇 |
1978年 | 39篇 |
1977年 | 39篇 |
1976年 | 35篇 |
1975年 | 48篇 |
1974年 | 37篇 |
1973年 | 58篇 |
1972年 | 61篇 |
1971年 | 65篇 |
1970年 | 26篇 |
1969年 | 31篇 |
1968年 | 29篇 |
1967年 | 20篇 |
1966年 | 18篇 |
排序方式: 共有2823条查询结果,搜索用时 15 毫秒
81.
82.
Dietary riboflavin deficiency is known to diminish malarial parasitemia. In this study, we determined whether imipramine and amitriptyline, drugs which inhibit riboflavin metabolism, have antimalarial efficacy. In addition, we evaluated whether these drugs, like other antimalarial agents, increase the hemolytic response to ferriprotoporphyrin IX (FP). The growth of Plasmodium falciparum (FCR3) in the absence and presence of these drugs (10 to 75 μM) was measured by determining (3H)hypoxanthine uptake by intraerythrocytic parasites for 48 h in RPMI 1640 medium. The uptake of (3H)hypoxanthine was significantly reduced in a dose-dependent manner by both imipramine and amitriptyline. The IC50 values of imipramine and amitriptyline at 48 h were 56 and 45 μM, respectively. Both drugs enhanced hemolysis induced by FP (10 or 20 μM). No hemolysis by these drugs was detected in the absence of FP. It is concluded that the tricyclic antidepressants, imipramine and amitriptyline, possess substantial antimalarial properties. 相似文献
83.
A note on optimality in lattice square designs 总被引:1,自引:0,他引:1
84.
Wintering site fidelity and movement patterns of Western Sandpipers Calidris mauri in the San Francisco Bay estuary 总被引:1,自引:0,他引:1
Western Sandpipers Calidris mauri are the most numerous shorebird species in the San Francisco Bay estuary during winter. A sample of 106 Western Sandpipers was captured in mist nets and radio-marked with 1-g transmitters to examine their wintering site fidelity and movements. Differences in distances moved, home range extent and core area size were examined by age, sex, season, site, time of day and tide. All birds remained in the south San Francisco Bay region during winter and exhibited strong site fidelity, with a mean home range of 22.0 km2 or only 8% of the study area. First-year birds had larger home ranges (26.6 ± 3.6 km2 ) than adults (17.2 ± 2.5 km2 ) in winter, but home range sizes of males and females were not significantly different in any period. Home range sizes were similar between seasons, but core areas were smaller in spring (6.3 ± 1.2 km2 ) than in early (9.6 ± 4.0 km2 ) or late (11.6 ± 1.6 km2 ) winter. Movements and home range size were similar for radio-marked birds located during day and night. The high degree of regional and local site fidelity demonstrated that the mixture of natural mud fiats and artificial salt ponds in southern San Francisco Bay provided sufficient resources for large wintering populations of Western Sandpipers. 相似文献
85.
1. We compared grazing by native noble crayfish ( Astacus astacus ) and the exotic signal crayfish ( Pacifastacus leniusculus ) on seedling or well-established macrophytes.
2. In a pool experiment, seedlings of emergent Scirpus lacustris and floating-leaved Potamogeton natans were heavily grazed by adult signal crayfish, whereas established plants of the same species sustained only minor damage.
3. In a preference experiment two submerged macrophytes ( Chara vulgaris and Elodea canadensis ), and both seedlings and established plants of S. lacustris and P. natans , were presented pairwise to signal and noble crayfish. There was no significant difference in preference by the two crayfish species. Chara vulgaris was preferred to all other plants presented, established plants of S. lacustris and P. natans were never preferred, and seedlings of P. natans were preferred to established P. natans .
4. An aquarium experiment was conducted in which the consumption of Chara by signal and noble crayfish was measured in relation to water temperature. Signal crayfish consumed significantly more Chara than noble crayfish, especially at higher temperatures.
5. Our results indicate that the signal crayfish is the more voracious grazer especially at higher temperatures. There may be negative effects on vegetation (emergents and floating-leaved, as well as submerged species) when the signal crayfish is introduced. Chara species are particularly susceptible, since they are preferred by crayfish and the genus includes a large number of rare species. Stocking of crayfish therefore could lead to the decline or removal of submerged species in order of crayfish feeding preferences and could prevent the spread or cause a decline of emergent and floating-leaved vegetation. 相似文献
2. In a pool experiment, seedlings of emergent Scirpus lacustris and floating-leaved Potamogeton natans were heavily grazed by adult signal crayfish, whereas established plants of the same species sustained only minor damage.
3. In a preference experiment two submerged macrophytes ( Chara vulgaris and Elodea canadensis ), and both seedlings and established plants of S. lacustris and P. natans , were presented pairwise to signal and noble crayfish. There was no significant difference in preference by the two crayfish species. Chara vulgaris was preferred to all other plants presented, established plants of S. lacustris and P. natans were never preferred, and seedlings of P. natans were preferred to established P. natans .
4. An aquarium experiment was conducted in which the consumption of Chara by signal and noble crayfish was measured in relation to water temperature. Signal crayfish consumed significantly more Chara than noble crayfish, especially at higher temperatures.
5. Our results indicate that the signal crayfish is the more voracious grazer especially at higher temperatures. There may be negative effects on vegetation (emergents and floating-leaved, as well as submerged species) when the signal crayfish is introduced. Chara species are particularly susceptible, since they are preferred by crayfish and the genus includes a large number of rare species. Stocking of crayfish therefore could lead to the decline or removal of submerged species in order of crayfish feeding preferences and could prevent the spread or cause a decline of emergent and floating-leaved vegetation. 相似文献
86.
Effects of elevated CO2 and/or O3 on growth, development and physiology of wheat (Triticum aestivum L.) 总被引:1,自引:0,他引:1
Two cultivars of spring wheat (Triticum aestivum L. cvs. Alexandria and Hanno) and three cultivars of winter wheat (cvs. Riband, Mercia and Haven) were grown at two concentrations of CO2 [ambient (355 pmol mol?1) and elevated (708 μmol mol?1)] under two O3 regimes [clean air (< 5 nmol mol?1 O3) and polluted air (15 nmol mol?1 O3 at night rising to a midday maximum of 75 nmol mol?1)] in a phytotron at the University of Newcastle-upon-Tyne. Between the two-leaf stage and anthesis, measurements of leaf gas-exchange, non-structural carbohydrate content, visible O3 damage, growth, dry matter partitioning, yield components and root development were made in order to examine responses to elevated CO2 and/or O3. Growth at elevated CO2 resulted in a sustained increase in the rate of CO2 assimilation, but after roughly 6 weeks' exposure there was evidence of a slight decline in the photosynthetic rate (c.-15%) measured under growth conditions which was most pronounced in the winter cultivars. Enhanced rates of CO2 assimilation were accompanied by a decrease in stomatal conductance which improved the instantaneous water use efficiency of individual leaves. CO2 enrichment stimulated shoot and root growth to an equivalent extent, and increased tillering and yield components, however, non-structural carbohydrates still accumulated in source leaves. In contrast, long-term exposure to O3 resulted in a decreased CO2 assimilation rate (c. -13%), partial stomatal closure, and the accumulation of fructan and starch in leaves in the light. These effects were manifested in decreased rates of shoot and root growth, with root growth more severely affected than shoot growth. In the combined treatment growth of O3-treated plants was enhanced by elevated CO2, but there was little evidence that CO2 enrichment afforded additional protection against O3 damage. The reduction in growth induced by O3 at elevated CO2 was similar to that induced by O3 at ambient CO2 despite additive effects of the individual gases on stomatal conductance that would be expected to reduce the O3 flux by 20%, and also CO2-induced increases in the provision of substrates for detoxification and repair processes. These observations suggest that CO2 enrichment may render plants more susceptible to O3 damage at the cellular level. Possible mechanisms are discussed. 相似文献
87.
JOHN B. MAHONEY JULIE A. McGHEE J. KNEELAND McNULTY 《The Journal of eukaryotic microbiology》1995,42(6):715-721
ABSTRACT. Alexandrium tamarense (Lebour) Balech (= Gonyaulax tamarensis Lebour) has been widely distributed and occasionally abundant in coastal waters of Long Island, New York in recent years. However, the distribution on the New Jersey coast has been sparse and this fact cannot be explained by this dinoflagellate's ability to migrate, or by its nutritional and physiological characteristics, or by the region's general suitability for phytoplankton. Therefore, the possibility that New Jersey coastal waters might be chemically exclusionary for A. tamarense seemed worth exploring. In a limited approach, we tested for water quality detrimental to the species in one New Jersey site (Great Bay) with a series of annual assays. Parsonage Creek, Long Island, New York, was assayed for comparison; this creek is assumed to have had at least marginal suitablity for A. tamarense , based on its reported long-term presence. Results provide tentative support for our working hypothesis, i.e. Great Bay chemical water quality is generally unfavorable for A. tamarense. Inhibition of A. tamarense growth, or culture decline, occurred in both assay series, but was substantially greater in Great Bay water. Inimical water quality was the most important factor distinguishing the two sites. Chelation with EDTA had greatest overall benefit in Great Bay assays, suggesting that lower availability of a natural chelator in the bay could be a secondary factor. Assay metal response is problematic, but we believe it permits speculation that essential metals could be partially limiting to A. tamarense in Great Bay, but would not be a critical regulator. 相似文献
88.
Induction of Antibiotic Resistance in Paramecium tetraurelia by the Bacterial Gene APH-3'-II 总被引:1,自引:0,他引:1
W. JOHN HAYNES KIT-YIN LING YOSHIRO SAIMI CHING KUNG 《The Journal of eukaryotic microbiology》1995,42(1):83-91
We have generated a transformation marker for Paramecium using a Paramecium expression vector (pPXV) and the open reading frame (ORF) of the bacterial antibiotic resistance gene aminoglycoside 3'-phosphotransferase-II (APH-3'-II or neor) from the transposon Tn5. The expression vector contained a small multiple cloning site between the 5' and 3' non-coding regions of the calmodulin gene, and Tetrahymena telomere sequences for the stability of the plasmid in Paramecium. After the neor ORF was inserted, the plasmid was referred to as pPXV-NEO. Delivery of approximately 10–20 picoliters of linearized PXV-NEO at > 2000 copies/pl into the macronucleus effected 100% transformation. Southern and Northern blot hybridization showed the presence of neor-specific DNA and RNA, respectively, in all of the transformed clones but not in the untransformed clones. The degree of resistance to G-418, and the concentrations of neor-specific DNA and neor-specific RNA in the clones were proportional to the concentration of the vector injected. We have demonstrated that when the linearized plasmid was injected into the macronucleus, the prokaryotic sequence conferred an antibiotic resistance to Paramecium despite codon-usage differences. 相似文献
89.
Influence of phytoplankton on the response of bacterioplankton growth to nutrient enrichment 总被引:1,自引:0,他引:1
1. Laboratory experiments were conducted to test the effect of nutrient enrichment on bacterioplankton growth in the presence and absence of phytoplankton. 2. In one series of experiments, bacterioplankton growth in terms of specific activity [3H-thymidine incorporation (cell number)?1] was greater in whole lake water samples than in samples from which phytoplankton had been removed by filtration (1.0 μm), regardless of the nutrient enrichments (control, NH+4 plus PO3-4 and mannitol). Organic C enhanced bacterioplankton growth in both whole and filtered lake water. 3. In another series of experiments (with the same nutrient enrichments as in the first experiment except that glucose replaced mannitol), bacterioplankton growth in whole lake water enriched with PO3-4 plus NH+4 and incubated in the light was greater than in two treatments designed to inhibit photosynthetic activity (+DCMU and dark). Bacterioplankton response to nutrient addition was greatest in the PO3-4 plus NH+4 enrichment under all three conditions (light +DCMU, and dark). 4. These results indicate that bacterioplankton growth could be directly limited by inorganic P and N when these elements are in short supply. Enhancement of bacterioplankton growth by phytoplankton occurs only under PO3-4 and NH+4 replete environments. 相似文献
90.
The effect of a foliar disease (rust) on the development of Gastrophysa viridula (Coleoptera: Chrysomelidae) 总被引:2,自引:0,他引:2
PAUL E. HATCHER NIGEL D. PAUL PETER G. AYRES JOHN B. WHITTAKER 《Ecological Entomology》1994,19(4):349-360
Abstract.
- 1 Gastrophysa viridula Degeer (Coleoptera: Chrysomelidae) and the pathogenic rust fungus Uromyces rumicis (Schum.) Wint. both occur on leaves of Rumex crispus L. and R.obtusifolius L. Individual stages of beetle development, and egg laying, were compared on healthy and infected leaves of each plant species in the laboratory. Oviposition choice was investigated in the field and laboratory.
- 2 Beetles reared on infected leaves of each species had greater larval mortality and slower development than those reared on healthy leaves. Although larvae feeding on infected leaves consumed up to 2.5 times more dry weight than those reared on healthy leaves, they had a lower relative growth rate and pupated at a lower weight. These changes were consistent with the reduced nutritive quality of rust-infected Rumex leaves.
- 3 Fecundity of beetles reared on infected leaves of both species was considerably reduced. Eggs laid by beetles feeding on infected R.crispus leaves also had a reduced viability.
- 4 The beetle developed consistently poorer on healthy R.crispus than on healthy R.obtusifolius throughout its life-cycle. Differences in larval performance were greater between host species than between infected and healthy leaves.
- 5 Oviposition was similar on infected and healthy R.crispus in both the laboratory and field. However, adults consumed less, and laid fewer eggs on infected than on healthy R.obtusifolius. The pattern of egg laying on different aged leaves was affected by rust infection: a greater proportion of eggs was laid on the older, infected leaves, than on the equivalent aged leaves on the healthy plants. Few larvae survived from eggs laid on rusted leaves in the field.