Barley yellow dwarf virus infectivity of alate aphid vectors in west Wales

Live trapping at 0.9 m of alate aphid vectors of barley yellow dwarf virus (BYDV) at Aberystwyth from 1970 to 1979 showed that ten species transmitted the virus to oat test plants. Conversion of percentage infective at 0.9 m to numbers infective based on continuous trapping at 1.2 m showed Rhopalosiphum padi and R. insertum to be the main vector species in most years, whilst Metopolophium dirhodum and Sitobion auenae were normally of minor importance. The data obtained suggest that epiphytotics of BYDV in autumn-sown cereals were caused by numerous infective vectors flying late in the year and transmitting severe strains of the virus. Evidence is presented that gynoparae and males of R. padi are involved in the autumn spread of BYDV and that three further aphid species, Anoecia corni, Metopolophium albidum and M. frisicum are BYDV vectors. The use of live and continuous trapping techniques in forecasting BYDV epiphytotics is discussed.     

Intranuclear Parasitism of the Ciliate Euplotes by a Trypanosomatid Flagellate

A trypanosomatid flagellate, Leptomonas sp., develops and multiplies in the macronucleus (only) of natural and laboratory-reared populations of the ciliate Euplotes. Up to 90% of the natural populations of Euplotes in our test pond had such nuclear infections. Laboratory infections were transmitted to this ciliate by feeding it liberated parasites. Paramecium resisted infections. All laboratory-induced infections were lethal to Euplotes, while control clones of the uninfected ciliates remained viable. This leptomonad, unlike Leptomonas karyophilus (found in Paramecium), shows no leishmanial forms in its several ciliate hosts and shows a varied pattern of locomotion.     

Comparative Studies on the Properties of Two Ferredoxins from Pisum sativum L.

Two ferredoxins in approximately equal amounts were isolatedfrom 3 week old Pisum sativum L. seedlings. Both ferredoxinshad identical absorption spectra with maxima at 276, 327, 424,and 468 nm in the oxidized state, and each possessed a single2Fe-2S active centre. The isoelectric points of the two ferredoxinswere both at pH 3·3, and mixtures could not be separatedby isoelectric focusing on polyacrylamide gels. The midpointredox potentials of the ferredoxins were close to –415mV, but they differed slightly in their biological activity.Ferredoxin I was slightly the more active of the two in catalysingNADP⁺ photoreduction by Pisum or Hordeum chloroplasts whereasferredoxin II was more active in catalysing the oxidative cleavageof pyruvate by extracts of Clostridium pasteurianum. Thoughthe molecular weights of the ferredoxins determined by ultracentrifugationwere the same within experimental error, the amino acid compositionsshowed marked differences. The N-terminal 40 amino acid residuesof ferredoxins I and II were determined by means of an automaticsequencer. There were 15 differences, suggesting that gene duplicationhad occurred early in evolutionary time. Ferredoxin I appearsto be more closely related to the other angiosperm ferredoxinssince it differed in only 6 positions compared with the correspondingsequence for Medicago sativa (alfalfa) ferredoxin. The ratioof the two ferredoxins in Pisum sativum was shown to be dependenton the age of the seedlings and environmental growth conditions.     

Euryhalinity of Palaeozoic articulate brachiopods

OMonotypic and very low diversity virgianid shell beds from the Upper Ordovician to Lower Silurian dolomites of North Greenland were formed in marginal marine quiet-water hypersaline environments. In the light of this evidence the salinity tolerances of other Palaeozoic articulate brachiopods is evaluated. There are only a small number of species apparently invading hypersaline or brackish environments, but it is significant considering that previously all articulate brachiopods were thought to be fully marine. Two types of occurrence are noted, those species specifically related to marginally marine environments, disappearing with the introduction of fully marine faunas, and the majority of species which extend their normal marine range into marginal conditions. No brachiopod species appears to have invaded very hypersaline or truly brackish conditions. No single group of articulate brachiopods specifically specialised in colonising marginal marine environments, apart from possibly the virgianid pentamerids. Palaeozoic, Upper Ordovician, Lower Silurian, Brachiopoda. Pentamerida, Virgianidae, Greenland, palaeoecology. hypersaline environments, brackish environments .     

Isolation and properties of a lectin from the roots of Pisum sativum (Garden pea)

Abstract The roots of pea (Pisum sativum L. ev. Feltham First) seedlings contained haemagglutinating activity and a protein which reacted with antibodies directed against pea seed lectin. This protein was shown to be present on the surface of root hairs and in the root cortical cells by immunofluorescence. Lectin (haemagglutinin) was purified from pea seedling roots by both immunoaffinity chromatography and affinity chromatography on Sephadex G-100. The pea root lectin was similar to the seed lectin when analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, and was antigenically identical: however, the isoelectric focussing band patterns of the proteins differed. The sugar specificity of the root lectin differed from that of the seed lectin, and the haemagglutinating activity of the root lectin was less than the seed lectin. These results are discussed with reference to the hypothesis that lectins mediate in the symbiotic association of legume and Rhizobium through their carbohydrate-binding properties.     

Phylogeny of Acomys spinosissimus (Rodentia, Muridae) from north Malawi and Tanzania: evidence from morphological and molecular analysis

New specimens of Acomys spinosissimus from northern Malawi and southern Tanzania were subjected to morphological, molecular (cytochrome b mitochondrial gene) and cytogenetical analysis in order to establish the phylogenetic position of these populations among A. spinosissimus, as well as their relationships with other species (A. subspinosus and A. wilsoni). The monophyly of the spinosissimus clade is supported by morphological and molecular phylogenies. Cytogenetics and morphology, but not the cytochrome b phylogeny show the possible existence of two sibling species within spinosissimus. Incongruences are noted between molecular and morphological trees, especially concerning the position of the Transvaal specimens. Two different biogeographic scenarios for the origin and dispersal of A. spinosissimus are discussed in the light of available palaeontological data.     

SENSORY PROPERTIES AND CONSUMER PERCEPTION OF 'HOME-MADE'AND COMMERCIAL DAIRY ICE CREAM

Sensory properties and consumer perception of 2 'home-made'and 4 commercial dairy ice creams were examined. Conventional profiling by a trained panel found that most samples differed significantly in all attributes, but distinction of 'home-made'from commercial products was limited to few attributes. Principal component analysis separated the 'home-made'products from the commercial ones mainly on the basis of differences in appearance, aroma, creamy flavors and textural attributes. A consumer group (n=105) recorded preconception of 'home-made'ice cream acceptance, then received the product samples, in unlabeled condition, for assessment of degree of liking (DOL) and for identification as 'home-made'or commercial. Internal preference mapping of the DOL ratings showed that consumers were more closely grouped around samples which had higher intensities of certain attributes. Higher 'denseness', 'vanilla'aroma and 'buttery'flavor, were associated with more liking for commercial products. The 'home-made'high fat sample, although liked, tended to be grouped with commercial products. More marked textural attributes of 'ice crystals'and 'breakage'aided identification of the low-fat 'home-made'formulation, which was less liked overall. Overall, differences between the 'home-made'and commercial samples were small but consumer perception of the term 'home-made'was viewed favorably for dairy ice cream.