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991.
A. Savage K.M. Leong D. Grobler J. Lehnhardt E.S. Dierenfeld E.F. Stevens C.P. Aebischer 《Zoo biology》1999,18(4):319-323
To date, there are no detailed reports of circulating levels of plasma α‐tocopherol and retinol for large samples of free‐ranging African elephants (Loxodonta africana). This survey study measured natural circulating levels of α‐tocopherol as a measure of vitamin E activity and retinol as an indicator of vitamin A activity, in 70 free‐ranging African elephants captured at Kruger National Park as part of a translocation program. Mean levels of α‐tocopherol and retinol were found to be 0.613 ± 0.271 μg/mL and 0.039± 0.007 μg/mL, respectively, and did not vary significantly across sex or age class. Elephants appear to normally have low circulating levels of both these nutrients compared with domestic herbivore species; values from healthy, free‐ranging elephant populations may provide useful data for assessing nutrient status of captive animals. Zoo Biol 18:319–323, 1999.© 1999 Wiley‐Liss, Inc. 相似文献
992.
This research studies dynamic culture for 3D tissue construct development with computational fluid dynamics. It proposes a mathematical model to evaluate the impact of flow rates and flow shear stress on cell growth in 3D constructs under perfusion. The modeling results show that dynamic flow, even at flow rate as low as 0.002 cm/s, can support much better mass exchange, higher cell number, and more even cell and nutrient distribution compared to static culture. Higher flow rate can further improve nutrient supply and mass exchange in the construct, promoting better nutritious environment and cell proliferation compared to lower flow rate. In addition, consideration of flow shear stress predicts much higher cell number in the construct compared to that without shear consideration. While the nutrient can dominate shear stress in influencing cell proliferation, the shear effect increases with flow rate. The proposed model helps tissue engineers better understand the cell-flow relationship at the molecular level during dynamic culture. 相似文献
993.
994.
Results of morphological studies supported by molecular analyses of chloroplast psbA‐trnH and partial petA‐psbJ DNA sequences indicate that Kaempferia candida cannot be retained in this genus. The species is here formally transferred to Curcuma and the name is typified. Notes on the species and colour plates are given. 相似文献
995.
Curcuma pambrosima (Zingiberaceae), a new species from central Vietnam, is described and illustrated. 相似文献
996.
Charles L. Crespi Toshiko Imamura Phaik-Mooi Leong Robert J. Fleischaker Henri Brunengraber William G. Thilly Donald J. Giard 《Biotechnology and bioengineering》1981,23(12):2673-2689
This report reviews recent progress in micro carrier technology at the Massachusetts Institute of Technology. This progress includes new understanding of the nutritional needs of high-cell density micro carrier cultures, the demonstration of cell growth on microcarriers in a hormone-supplemented, serum-free medium, and the continuous cell propagation of epithelial cell types in microcarrier culture by medium modification that permits efficient bead-to bead transfer. Also, a technique for obtaining large quantities of mitotic cells by selective detachment from microcarriers is reported. Finally, recent progress in interferon production from human foreskin fibroblasts grown on microcarriers is outlined: Our improvements in the interferon “super induction” process have increased the interferon yield per cell fivefold to tenfold. 相似文献
997.
998.
Mateusz Rytelewski Adrian Buensuceso Hon S. Leong Bonnie J. Deroo Ann F. Chambers James Koropatnick 《Journal of visualized experiments : JoVE》2015,(96)
Due to the high level of heterogeneity and mutations inherent in human cancers, single agent therapies, or combination regimens which target the same pathway, are likely to fail. Emphasis must be placed upon the inhibition of pathways that are responsible for intrinsic and/or adaptive resistance to therapy. An active field of investigation is the development and testing of DNA repair inhibitors that promote the action of, and prevent resistance to, commonly used chemotherapy and radiotherapy. We used a novel protocol to evaluate the effectiveness of BRCA2 inhibition as a means to sensitize tumor cells to the DNA damaging drug cisplatin. Tumor cell metabolism (acidification and respiration) was monitored in real-time for a period of 72 hr to delineate treatment effectiveness on a minute by minute basis. In combination, we performed an assessment of metastatic frequency using a chicken embryo chorioallantoic membrane (CAM) model of extravasation and invasion. This protocol addresses some of the weaknesses of commonly used in vitro and in vivo methods to evaluate novel cancer therapy regimens. It can be used in addition to common methods such as cell proliferation assays, cell death assays, and in vivo murine xenograft studies, to more closely discriminate amongst candidate targets and agents, and select only the most promising candidates for further development. 相似文献
999.
1000.
The statistical predictions of Newtonian and special-relativistic mechanics, which are calculated from an initially Gaussian ensemble of trajectories, are compared for a low-speed scattering system. The comparisons are focused on the mean dwell time, transmission and reflection coefficients, and the position and momentum means and standard deviations. We find that the statistical predictions of the two theories do not always agree as conventionally expected. The predictions are close if the scattering is non-chaotic but they are radically different if the scattering is chaotic and the initial ensemble is well localized in phase space. Our result indicates that for low-speed chaotic scattering, special-relativistic mechanics must be used, instead of the standard practice of using Newtonian mechanics, to obtain empirically-correct statistical predictions from an initially well-localized Gaussian ensemble. 相似文献