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691.
692.
Histochemistry of ATPases 总被引:2,自引:0,他引:2
693.
Insulin binding to brain capillaries is reduced in genetically obese, hyperinsulinemic Zucker rats 总被引:1,自引:0,他引:1
In order to study the role of plasma insulin in regulating the binding of insulin to the endothelium of the blood-brain barrier (BBB), insulin binding to a purified preparation of brain capillaries was measured in both genetically obese Zucker rats and lean Zucker controls. We found a reduction of 65% in brain capillary insulin binding site number in the obese compared to lean rats with no change in receptor affinity. Furthermore, specific insulin binding to brain capillaries was negatively correlated (p less than 0.05) to the plasma insulin level, suggesting a role for plasma insulin in regulating insulin binding. A similar relationship was observed between insulin receptor number in liver membranes and the plasma insulin level. We conclude that obese, hyperinsulinemic Zucker rats exhibit a reduction in the number of BBB insulin receptors, which parallels the reduction seen in other peripheral tissues. Since insulin receptors have been hypothesized to participate in the transport of insulin across the BBB, the reduction observed in the obese rats may account for the decrease in cerebrospinal fluid insulin uptake previously demonstrated in these animals. 相似文献
694.
695.
How does a plant cell sense and respond to the status of its cell wall? Intercourse between cell wall and cytoplasm has long been supposed to involve arabinogalactan proteins, in part because many of them are anchored to the plasma membrane. Disrupting arabinogalactan proteins has recently been shown to disrupt the array of cortical microtubules present just inside the plasma membrane, implying that microtubules and arabinogalactan proteins interact. In this article, we assess possibilities for how this interaction might be mediated. First, we consider microdomains in the plasma membrane (lipid rafts), which have been alleged to link internal and external regions of the plasma membrane; however, the characteristics and even the existence of these domains remains controversial. Next, we point out that disrupting the synthesis of cellulose also can disrupt microtubules and consider whether arabinogalactan proteins are part of a network linking microtubules and nascent microfibrils. Finally, we outline several signaling cascades that could transmit information from arabinogalactan proteins to microtubules through channels of cellular communication. These diverse possibilities highlight the work that remains to be done before we can understand how plant cells communicate across their membranes. 相似文献
696.
Association of Plant p40 Protein with Ribosomes Is Enhanced When Polyribosomes Form during Periods of Active Tissue Growth 下载免费PDF全文
p40s are acidic proteins of eukaryotic cells occurring either free in the cytoplasm or in association with ribosomes, the latter occurring in both monosomes and polysomes. p40s may play a role in the regulation of protein synthesis, although the exact mechanism is not known. Leaves of all 10 plant species examined here, including both monocots and dicots, contained proteins detected on immunoblots with Arabidopsis thaliana p40 antiserum. The number and apparent size of the protein bands were variable even among closely related species. Abundance of p40 relative to ribosomal content during soybean (Glycine max L.) seed germination and during seed and leaf development was examined. p40 abundance correlated with periods of active tissue growth and high polysome content. The plant growth regulator indole acetic acid caused an increase in polysome formation in etiolated pea (Pisum sativum L.) plants and a concomitant recruitment of p40 into polysomes. Subcellular localization at the microscopy level indicated that the pattern of p40 staining is very similar to that for RNA, except that p40 is excluded from the nucleus. These data suggest that p40 is an accessory protein of the ribosome that might play a role in plant growth and development. 相似文献
697.
698.
Depolarization spectrum of diffracted light from muscle fiber. The intrinsic anisotropy component. 下载免费PDF全文
The depolarization signal of the diffraction patterns from muscle fibers includes information that differs from that of transmission birefringence experiments. Although both the birefringence studies and the phase shift studies of Yeh et al. (Yeh, Y, and G. Pinsky, 1983, Biophys. J., 42:83-90; Yeh, Y., M. E. Corcoran, R. J. Baskin, and R. L. Lieber, 1983, Biophys. J., 44:343-351) include inseparable intrinsic and form contributions, the present analysis shows that the magnitude of the E-field components of diffracted light is affected only by the intrinsic contribution. We have analyzed the amplitude portion of the data of which the phase shift portion had previously been reported (Yeh, Y., M. E. Corcoran, R. J. Baskin, and R. L. Lieber, 1983, Biophys. J., 44:343-351). For the relaxed-to-rigor transition, these field amplitudes also exhibit changes when ATP concentration is decreased. The observed decrease in optical depolarization upon rigor is consistent with the idea that optically anisotropic elements move away from the myosin thick filament under such conditions. 相似文献
699.
Somatostatin in epithelial cells of intestinal mucosa is present primarily as somatostatin 28 总被引:2,自引:0,他引:2
Region-specific antisera to [Tyr14]-SS28(1-14) were used to identify cells containing immunoreactivity to the SS28(1-14) fragment of somatostatin 28 (SS28) in gastric and intestinal mucosal epithelium and in pancreatic islets by immunoperoxidase staining. Radioimmunoassay with iodinated [Tyr14]-SS28(1-14) identified one antiserum (F4) to SS28(1-14) that cross-reacted equally with SS28(1-12), SS28(1-14) and SS28. Two other antisera (F3 and F8) to SS28(1-14) did not cross-react with SS28(1-12) and showed insignificant cross-reactivity to SS28. Immunostaining results showed that F4 stained the same cells that reacted with antiserum AS-10, which is specific for the cyclic tetradecapeptide somatostatin, SS28(15-28). Antisera F3, F4, and F8 all reacted with islet D cells and with somatostatin cells in the antral mucosa. However, only antiserum F4 detected immunoreactivity in mucosal epithelial cells; F3 and F8 did not bind to these cells. After sections of intestine were exposed to trypsin, however, epithelial cells containing immunoreactivity to SS28(1-14) were detected in intestinal mucosa with antisera F3 and F8. These results were obtained for duodenum, jejunum, ileum, and colon, but most of the epithelial cells with immunoreactivity to SS28(1-14) were in the duodenum. Both radioimmunoassay and immunostaining results suggest that F3 and F8 bind to a region of SS28(1-14) that is unavailable to antibodies in the intact SS28 molecule.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
700.
Molecular coevolution among cryptically simple expansion segments of eukaryotic 26S/28S rRNAs 总被引:16,自引:4,他引:12
The set of "expansion segments" of any eukaryotic 26S/28S ribosomal RNA
(rRNA) gene is responsible for the bulk of the difference in length between
the prokaryotic 23S rRNA gene and the eukaryotic 26S/28S rRNA gene. The
expansion segments are also responsible for interspecific fluctuations in
length during eukaryotic evolution. They show a consistent bias in base
composition in any species; for example, they are AT rich in Drosophila
melanogaster and GC rich in vertebrate species. Dot-matrix comparisons of
sets of expansion segments reveal high similarities between members of a
set within any 28S rRNA gene of a species, in contrast to the little or
spurious similarity that exists between sets of expansion segments from
distantly related species. Similarities among members of a set of expansion
segments within any 28S rRNA gene cannot be accounted for by their
base-compositional bias alone. In contrast, no significant similarity
exists within a set of "core" segments (regions between expansion segments)
of any 28S rRNA gene, although core segments are conserved between species.
The set of expansion segments of a 26S/28S gene is coevolving as a unit in
each species, at the same time as the family of 28S rRNA genes, as a whole,
is undergoing continual homogenization, making all sets of expansion
segments from all ribosomal DNA (rDNA) arrays in a species similar in
sequence. Analysis of DNA simplicity of 26S/28S rRNA genes shows a direct
correlation between significantly high relative simplicity factors (RSFs)
and sequence similarity among a set of expansion segments. A similar
correlation exists between RSF values, overall rDNA lengths, and the
lengths of individual expansion segments. Such correlations suggest that
most length fluctuations reflect the gain and loss of simple sequence
motifs by slippage-like mechanisms. We discuss the molecular coevolution of
expansion segments, which takes place against a background of slippage-like
and unequal crossing-over mechanisms of turnover that are responsible for
the accumulation of interspecific differences in rDNA sequences.
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