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81.
82.
Neurochemical Research - MicroRNAs (miRNAs) have been implicated in nerve injury and demyelination; however, their functions in peripheral nerves remain unclear. To determine the potential... 相似文献
83.
Jong In Kim Jin Young Huh Jee Hyung Sohn Sung Sik Choe Yun Sok Lee Chun Yan Lim Ala Jo Seung Bum Park Weiping Han Jae Bum Kim 《Molecular and cellular biology》2015,35(10):1686-1699
In obesity, adipocyte hypertrophy and proinflammatory responses are closely associated with the development of insulin resistance in adipose tissue. However, it is largely unknown whether adipocyte hypertrophy per se might be sufficient to provoke insulin resistance in obese adipose tissue. Here, we demonstrate that lipid-overloaded hypertrophic adipocytes are insulin resistant independent of adipocyte inflammation. Treatment with saturated or monounsaturated fatty acids resulted in adipocyte hypertrophy, but proinflammatory responses were observed only in adipocytes treated with saturated fatty acids. Regardless of adipocyte inflammation, hypertrophic adipocytes with large and unilocular lipid droplets exhibited impaired insulin-dependent glucose uptake, associated with defects in GLUT4 trafficking to the plasma membrane. Moreover, Toll-like receptor 4 mutant mice (C3H/HeJ) with high-fat-diet-induced obesity were not protected against insulin resistance, although they were resistant to adipose tissue inflammation. Together, our in vitro and in vivo data suggest that adipocyte hypertrophy alone may be crucial in causing insulin resistance in obesity. 相似文献
84.
Jong-Yil Chai Woon-Mok Sohn Byoung-Kuk Na Jong-Bok Park Hoo-Gn Jeoung Eui-Hyug Hoang Thi Thi Htoon Htay Htay Tin 《The Korean journal of parasitology》2015,53(5):619-625
The present study was performed to determine the infection status of swamp eels with Gnathostoma sp. larvae in Myanmar. We purchased total 37 Asian swamp eels, Monopterus albus, from a local market in Yangon in June and December 2013 and 2014. All collected eels were transferred with ice to our laboratory and each of them was examined by the artificial digestion technique. A total of 401 larval gnathostomes (1-96 larvae/eel) were detected in 33 (89.2%) swamp eels. Most of the larvae (n=383; 95.5%) were found in the muscle. The remaining 18 larvae were detected in the viscera. The advanced third-stage larvae (AdL3) were 2.3-4.4 mm long and 0.25-0.425 mm wide. The characteristic head bulb (0.093 × 0.221 mm in average size) with 4 rows of hooklets, muscular long esophagus (1.025 mm), and 2 pairs of cervical sacs (0.574 mm) were observed by light microscopy. The average number of hooklets in the 1st, 2nd, 3rd, and 4th rows was 41, 45, 48, and 51, respectively. As scanning electron microscopic findings, the characteristic 4-5 rows of hooklets on the head bulb, a cervical papilla, tegumental spines regularly arranged in the transverse striations, and an anus were well observed. Based on these morphological characters, they were identified as the AdL3 of Gnathostoma spinigerum. By the present study, it has been confirmed for the first time that Asian swamp eels, M. albus, from Yangon, Myanmar are heavily infected with G. spinigerum larvae. 相似文献
85.
Tae Hoon Kwon Hyunwoo Jung Eun Jeong Cho Ji Hoon Jeong Uy Dong Sohn 《Molecules and cells》2015,38(7):616-623
P2 receptors are membrane-bound receptors for extracellular nucleotides such as ATP and UTP. P2 receptors have been classified as ligand-gated ion channels or P2X receptors and G protein-coupled P2Y receptors. Recently, purinergic signaling has begun to attract attention as a potential therapeutic target for a variety of diseases especially associated with gastroenterology. This study determined the ATP and UTP-induced receptor signaling mechanism in feline esophageal contraction. Contraction of dispersed feline esophageal smooth muscle cells was measured by scanning micrometry. Phosphorylation of MLC20 was determined by western blot analysis. ATP and UTP elicited maximum esophageal contraction at 30 s and 10 μM concentration. Contraction of dispersed cells treated with 10 μM ATP was inhibited by nifedipine. However, contraction induced by 0.1 μM ATP, 0.1 μM UTP and 10 μM UTP was decreased by , chelerythrine, ML-9, PTX and GDPβS. Contraction induced by 0.1 μM ATP and UTP was inhibited by Gαi3 or Gαq antibodies and by PLCβ1 or PLCβ3 antibodies. Phosphorylated MLC20 was increased by ATP and UTP treatment. In conclusion, esophageal contraction induced by ATP and UTP was preferentially mediated by P2Y receptors coupled to Gαi3 and G q proteins, which activate PLCβ1 and PLCβ3. Subsequently, increased intracellular Ca2+ and activated PKC triggered stimulation of MLC kinase and inhibition of MLC phosphatase. Finally, increased pMLC20 generated esophageal contraction. U73122相似文献
86.
87.
Bailey K Cevik V Holton N Byrne-Richardson J Sohn KH Coates M Woods-Tör A Aksoy HM Hughes L Baxter L Jones JD Beynon J Holub EB Tör M 《Molecular plant-microbe interactions : MPMI》2011,24(7):827-838
RPP5 is the seminal example of a cytoplasmic NB-LRR receptor-like protein that confers downy mildew resistance in Arabidopsis thaliana. In this study, we describe the cloning and molecular characterization of the gene encoding ATR5(Emoy2), an avirulence protein from the downy mildew pathogen Hyaloperonospora arabidopsidis isolate Emoy2. ATR5(Emoy2) triggers defense response in host lines expressing the functional RPP5 allele from Landsberg erecta (Ler-0). ATR5(Emoy2) is embedded in a cluster with two additional ATR5-like (ATR5L) genes, most likely resulting from gene duplications. ATR5L proteins do not trigger RPP5-mediated resistance and the copy number of ATR5L genes varies among H. arabidopsidis isolates. ATR5(Emoy2) and ATR5L proteins contain a signal peptide, canonical EER motif, and an RGD motif. However, they lack the canonical translocation motif RXLR, which characterizes most oomycete effectors identified so far. The signal peptide and the N-terminal regions including the EER motif of ATR5(Emoy2) are not required to trigger an RPP5-dependent immune response. Bioinformatics screen of H. arabidopsidis Emoy2 genome revealed the presence of 173 open reading frames that potentially encode for secreted proteins similar to ATR5(Emoy2), in which they share some motifs such as EER but there is no canonical RXLR motif. 相似文献
88.
The molecular mechanisms involved in the ability of yeast cells to adapt and respond to oxidative stress are of great interest
to the pharmaceutical, medical, food, and fermentation industries. In this study, we investigated the time-dependent, cellular
redox homeostasis ability to adapt to menadione-induced oxidative stress, using biochemical and proteomic approaches in Saccharomyces cerevisiae KNU5377. Time-dependent cell viability was inversely proportional to endogenous amounts of ROS measured by a fluorescence
assay with 2′,7′-dichlorofluorescin diacetate (DCFHDA), and was hypersensitive when cells were exposed to the compound for
60 min. Morphological changes, protein oxidation and lipid peroxidation were also observed. To overcome the unfavorable conditions
due to the presence of menadione, yeast cells activated a variety of cell rescue proteins including antioxidant enzymes, molecular
chaperones, energy-generating metabolic enzymes, and antioxidant molecules such as trehalose. Thus, these results show that
menadione causes ROS generation and high accumulation of cellular ROS levels, which affects cell viability and cell morphology
and there is a correlation between resistance to menadione and the high induction of cell rescue proteins after cells enter
into this physiological state, which provides a clue about the complex and dynamic stress response in yeast cells. 相似文献
89.
90.
The New World genus Eucalantica Busck, 1904 is reviewed. It comprises seven species, six of which are described as new: Eucalantica costaricae Sohn & Nishida, sp. n., Eucalantica ehecatlella Sohn & Nishida, sp. n., Eucalantica icarusella Sohn & Nishida, sp. n., Eucalantica powelli Sohn, sp. n., and Eucalantica pumila Sohn, sp. n., all five from Costa Rica; Eucalantica vaquero Sohn, sp. n. from southern USA and Mexico. The type species, Eucalantica polita (Walsingham, 1881), is redescribed and a lectotype and two paralectotypes are designated. Illustrations and keys based on the forewing patterns and the genitalia of each sex are provided. Our review suggests that there remains an undiscovered high diversity of Eucalantica in the tropical highlands of Central America. 相似文献