水库对投饵网箱养鱼的负荷力

本文用１８个１４．３ｍ￣３的围隔组成的围隔群,以鲤为材料,研究了水库对投饵网箱养鱼的负荷力。实验中观测了水温、透明度、ｐＨ值、溶氧、化学耗氧量、生化需氧量与非离子氨等环境因素的变化。结束时将这些变化与我国渔业水质标准相比较,查明该负荷力约为３０００ｋｇ·ｈａ￣（－１）,建议增加２５－３５％的安全储备,以最大载鱼量１８００－２３００ｋｇ·ｈａ￣（－１）作为推荐值。     

水分胁迫对小麦光系统Ⅱ的影响

水分胁迫可降低小麦叶绿体的室温荧光产量和Ｍｇ２＋ 在两个光系统间的调节能力、叶片的可变荧光产量、可变荧光猝灭速率以及荧光上升互补面积,表明光系统Ⅱ受到了伤害。光系统Ⅱ氧化侧的人工电子供体ＤＰＣ能部分恢复受到抑制的叶绿体可变荧光,说明水分胁迫对光系统Ⅱ的损伤部位不仅位于氧化侧,也可能在反应中心上     

Isolation and Refined Regional Mapping of Expressed Sequences from Human Chromosome 21

To increase candidate genes from human chromosome 21 for the analysis of Down syndrome and other genetic diseases localized on this chromosome, we have isolated and studied 9 cDNA clones encoded by chromosome 21. For isolating cDNAs, single-copy microclones from a chromosome 21 microdissection library were used in direct screening of various cDNA libraries. Seven of the cDNA clones have been regionally mapped on chromosome 21 using a comprehensive hybrid mapping panel comprising 24 cell hybrids that divide the chromosome into 33 subregions. These cDNA clones with refined mapping positions should be useful for identification and cloning of genes responsible for the specific component phenotypes of Down syndrome and other diseases on chromosome 21, including progressive myoclonus epilepsy in 21q22.3.     

胎盘型谷胱甘肽S－转移酶基因在胃癌中的表达

用Ｄｉｇ－ＧＳＴ－πｃＤＮＡ探针分子杂交方法,检测了正常胃组织,胃癌及相应癌旁正常组织中ＧＳＴ－πＤＮＡ和ＧＳＴ－πＲＮＡ水平,发现ＧＳＴ－πＤＮＡ水平没有明显变化,而ＧＳＴ－πＲＮＡ在８例胃癌组织中有６例高于正常胃组织,在１２例低分化腺癌中有７例癌旁正常组织高于相应癌组织,表明ＧＳＴ－π基因表达增加与胃癌有关,而且早于细胞形态的变化。     

鲑鱼生长激素基因分泌型表达质粒的构建

生长激素(GH)是动物垂体前叶分泌的一种多肽类激素.应用分子重组及PCR等技术,构建了一种鲑鱼生长激素基因分泌型表达质粒pOsGH153,使编码鲑鱼生长激素成熟肽的序列克隆在大肠杆菌分泌型表达载体PIN-Ⅲ-ompA内,直接位于编码大肠杆菌外膜蛋白A信号肽序列的下游,在Lpp-Lac杂合启动子控制下,经IPTG诱导,分子量约23 000的鲑鱼生长激素在大肠杆菌中获得高效表达,该产物具有天然鲑鱼生长激素的免疫活性,直接分泌到细胞周质,而信号肽被自动剪除.     

埃及血吸虫毛蚴表皮板的观察

埃及血吸虫毛蚴表皮板共有4排,各排数目为6:9:4:3。除第1排之外,其他各排的表皮板均由无纤毛的嵴所分隔,在第1与第2排之间的环嵴中,除第1排各板底边中央小凹处各有1感受器外,尚可见4对感受器。第2与第3排的环嵴内可见16—20个感受器。此外,于体之两侧第3排表皮板间各见1个排泄孔。顶突似六边形,于其两侧各有1个侧腺开口,其内侧可见14个感受器。本文在讨论中与前人对本虫毛蚴表皮板的描述做了简单比较。     

分离自赤豆的青霉新种

本文报道青霉属一个新种,分离自赤豆,特点是分生孢子无色,命名为无色青霉(Penicillium incoloratum sp.nov.).模式和来自模式的活培养物都保存在中国科学院微生物研究所。     

抗氧化剂对苜蓿原生质体早期培养的影响

研究发现,分离原生质体的酶解脱壁处理可以诱导苜蓿细胞产生活性氧。培养基中添加抗氧化剂,有助于提高培养原生质体的分裂频率,缓解褐化现象的出现。经紫外照射处理的培养基不利于苜蓿原生质体的生长和分裂,添加抗氧化剂后,紫外辐射所引起的不良效应则被抵消。因而,通过抗氧化剂对活性氧的清除,有助于早期原生质体的培养。     

大黄素对豚鼠结肠带平滑肌细胞电和收缩性能的影响

联合应用平滑肌肌力张力测量技术和细胞内微电极记录技术,同步地现测豚鼠结肠带平滑肌自发的肌源性电活动和力学活动,研究了大黄素的药物作用。大黄素能缩短膜电位的波动周期,从而缩短峰电位集簇发放的周期;相应地,可使平滑肌的分节律收缩加快,幅值指数升高。大黄素又能促使细胞膜电位自发的周期性波动的出现,导致峰电位的集簇发放;相应地,可使强直收缩转化为分节律收缩,即促进收缩形式向有利于肠道推进功能的方向转化。以上结果表明,大黄素能有效地提高豚鼠结肠带平滑肌细胞的电兴奋性和收缩功能,并且对其电学和力学活动的影响之间有明确的对应关系。     

The inhibitory effect of transthyretin gene on growth of human hepatoma cells

Transthyretin(TTR) gene was highly expressed in normal liver and it has been found to be deleted in part of DNA samples from human hepatic cancer.Its mRNA expression was suppressed in most hepatoma samples.In order to study the biological effect of TTR gene on the growth of hepatoma cells,a recombinant vector containing TTR cDNA was constructed by pCMV,then it was transfected into hepatoma cell lines SMMC-7721 and Q3.It has been demonstrated that the inhibition of growth rate of TTR cDNA transfected hepatoma cells was about 50% in strength compared with that of the control.This inhibition was further enhanced when the transfected hepatoma cells were treated with all-trans retinoic acid.Hepatoma cells of cell lines PLC/PRF/5,SMMC-7721 and Q3 as well as hepatoma cells SMMC-7721 transfected with pCMV or pCMV-TTR were analyzed for TTR expression by Northern hybridization.The low level of TTR expression was found in both hepatoma cell lines and in SMMC-7721 cells transfected with pCMV alone.However,a remarkable TTR mRNA expression was observed in hepatoma SMMV-7721 cells transfected with pCMV-TTR.It seems possible that TTR gene might be a candidate of cancer suppressor gene for human hepatic cancer.