后侧手术入路、锁定钢板固定治疗胫骨平台后髁骨折的临床疗效

目的：探讨采用膝关节后内和后外侧切口、锁定钢板固定治疗胫骨平台后髁骨折的临床疗效。方法：选取2009年1月～2011年12月来我院治疗的13例胫骨平台后髁骨折患者作为研究对象,所有患者采用后内和后外侧切口、锁定钢板固定治疗,观察术后骨折愈合情况,采用Rasmussen放射学评分评定骨折复位情况,采用HSS评分系统评定膝关节功能疗效。结果：术后随访6～24个月,平均13．6个月,所有患者骨折均于16周内愈合。Rasmussen放射学评分15～18分,平均17．4分,其中优8例,良3例,可2例,优良率为84．7％。末次随访时膝关节功能HSS评分73~97分,平均91．4分,其中获优7例,良5例,可1例,优良率为92．3％。结论：采用膝关节后内和（或）后外侧切口、锁定钢板固定治疗胫骨平台后髁骨折具有操作简单、显露清晰、直视下复位骨折及关节面、固定牢靠、利于早期功能锻炼、临床疗效确切、并发症少等优点。     

菌糠二次利用栽培黑木耳试验研究

为充分利用营养没有得到充分吸收的栽培过食用菌的废弃菌糠、明确在牡丹江地区再利用食用菌菌糠生产黑木耳的适宜比例,以灵芝和鲍鱼菇的废弃菌糠为试验材料,进行不同配方的对比试验。试验结果表明：黑木耳培养料中灵芝菌糠体积分数为30%时栽培效果最佳,显著优于参入鲍鱼菇菌糠的各种培养料配方（P〈0.01）。因此,可以利用灵芝菌糠废料作为替代料栽培黑木耳使用。     

晚期早产儿颅内出血的相关因素分析

目的：探讨晚期早产儿颅内出血的相关因素,指导晚期早产儿颅内出血的防治。方法：2011年9月至2012年8月我院收治晚期早产儿253例,其中有210例行头颅MRI检查,以经头颅MRI检查确诊颅内出血30例为ICH组,同时随机抽取同期住院的经头颅MRI证实无颅内出血晚期早产儿60例作为对照组。应用SPSS 17.0进行统计学分析。结果：1.ICH组产前激素应用率显著低于对照组（P〈0.05）。2.ICH组经阴分娩、胎膜早破、代谢性酸中毒发生率显著高于对照组（P〈0.05）。3.Logistic回归分析显示产前激素是颅内出血的保护因素（P〈0.05）,而经阴分娩、胎膜早破（P〈0.01）、代谢性酸中毒（P〈0.05）是颅内出血的危险因素。结论：产前应用激素是晚期早产儿颅内出血的保护因素,经阴分娩、胎膜早破、代谢性酸中毒是晚期早产儿颅内出血高危因素。     

蚯蚓提取物对家蚕中肠氧化损伤、总抗氧化能力及抗氧化酶含量的影响

【目的】研究蚯蚓提取物对家蚕Bombyx mori中肠组织氧化应激及抗氧化酶含量的影响,探究蚯蚓提取物的抗氧化功能。【方法】分别给家蚕5龄幼虫饲喂蚯蚓(赤子爱胜蚓Eisenia foetida)提取液原液的50, 100和200倍稀释液处理后的桑叶,测定处理6 d后家蚕中肠组织中氧化损伤产物丙二醛(MDA)和乳酸脱氢酶(LDH)的含量;利用qRT-PCR检测家蚕中肠组织中抗氧化酶关键基因(Cat,Sod和GSH-Px)的mRNA表达水平;同时采用ELISA法检测中肠组织中抗氧化酶[过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)]含量及总抗氧化能力(T-AOC)。【结果】与对照组相比,50, 100和200倍稀释组家蚕中肠组织中MDA, CAT, SOD和GSH-Px含量及T-AOC均差异不显著,200倍稀释组LDH含量显著降低了9.85%,100倍稀释组中肠中Cat和GSH-Px的表达量均显著高于对照组,3个稀释组中Sod的表达量均显著高于对照组。【结论】添食蚯蚓提取物可通过降低家蚕中肠LDH含量、提高抗氧化酶基因的表达水平进而提高家蚕的抗氧化能力。     

Proximity association in polygynous western black crested gibbons (Nomascus concolor jingdongensis): network structure and seasonality

We investigated the structure and seasonality of the proximity network in a group of polygynous western black crested gibbons (Nomascus concolor) using social network analysis. The spatial proximity changed seasonally and was affected by temperature and rainfall. Preferred proximity association was not distributed randomly among individuals. Kinship was one explanation for the social structure, as offspring preferred to maintain close proximity with their mothers. The proximity of infants to mothers decreased with age, and independent offspring had lower proximity to mothers than dependent ones. We found that the adult male had different proximity relationships with two different adult females. The frequency of proximity between the male and the infant-carrying female was significantly higher than that between the male and the female who had immigrated carrying one offspring of uncertain paternity into the group. Infanticide avoidance and/or predation protection for dependent infants might explain the proximity relationship differences. Temperature influenced group proximity association, with individual proximity increasing in the cold months and decreasing in the hot months. Group proximity decreased in months with higher anthropogenic disturbance.     

中国鸟类亚种新记录——黑冠黄鹎

2010年12月和2011年4月,分别在广西西北部百色市右江区和靖西县古龙山自然保护区拍摄和观察到黑冠黄鹎(Pycnonotus flaviventris)的johnsoni亚种.其喉部为红色,与中国其他记录亚种区别明显,属中国鸟类亚种的新记录.该文亦初步讨论了该亚种的分类和分布.     

番茄果实成熟过程中番茄红素含量及合成相关基因表达的分析

以2个高代自交系粉果番茄MLK1和红果番茄FL1为材料,利用实时荧光定量PCR技术及色差仪法,对果实成熟过程中4个时期的番茄红素含量分析及八氢番茄红素合成酶(Psy1和Psy2)和番茄红素环化酶(Lcy)基因的表达进行研究。结果表明,在番茄果实成熟的过程中,番茄红素的含量也逐渐增高,在完熟期达到最高,且红果中的含量高于粉果中的。在2个番茄品种果实不同部位中,Psy1、Psy2和Lcy基因在果实逐渐成熟的过程中转录水平均逐渐增加,在完熟期表达量最高,且红果FL1中的表达量高于粉果MLK1表达量,果实中Psy基因的表达量高于Lcy基因的表达量。     

淡足侧沟茧蜂寄生对甜菜夜蛾幼虫血淋巴总糖、蛋白质及虫体脂质含量的影响

为探讨淡足侧沟茧蜂Microplitis pallidipes Szepligeti调控寄主的生理机制,测定了淡足侧沟茧蜂寄生后,甜菜夜蛾Spodoptera exigua(Hübner)幼虫血淋巴总糖、蛋白质及虫体脂类含量的变化。研究结果表明,寄生后连续5 d的观察时间内,甜菜夜蛾幼虫血淋巴总糖含量从寄生后第1天开始就高于未寄生寄主幼虫,且在寄生后第2至5天达到显著水平;除寄生后第3天外,被寄生寄主幼虫血淋巴总蛋白质含量始终低于未寄生幼虫,且在寄生后第1、4、5天达到显著水平;甜菜夜蛾被寄生后,虫体脂质含量始终高于未寄生幼虫,且从寄生后第2天开始达到显著水平。本研究揭示,淡足侧沟茧蜂寄生刺激了寄主甜菜夜蛾幼虫血淋巴总糖合成,但抑制了其蛋白质合成,同时也刺激了甜菜夜蛾虫体脂质合成。     

Midday stomatal conductance is more related to stem rather than leaf water status in subtropical deciduous and evergreen broadleaf trees

Midday depressions in stomatal conductance (g_s) and photosynthesis are common in plants. The aim of this study was to understand the hydraulic determinants of midday g_s, the coordination between leaf and stem hydraulics and whether regulation of midday g_s differed between deciduous and evergreen broadleaf tree species in a subtropical cloud forest of Southwest (SW) China. We investigated leaf and stem hydraulics, midday leaf and stem water potentials, as well as midday g_s of co‐occurring deciduous and evergreen tree species. Midday g_s was correlated positively with midday stem water potential across both groups of species, but not with midday leaf water potential. Species with higher stem hydraulic conductivity and greater daily reliance on stem hydraulic capacitance were able to maintain higher stem water potential and higher g_s at midday. Deciduous species exhibited significantly higher stem hydraulic conductivity, greater reliance on stem capacitance, higher stem water potential and g_s at midday than evergreen species. Our results suggest that midday g_s is more associated with midday stem than with leaf water status, and that the functional significance of stomatal regulation in these broadleaf tree species is probably for preventing stem xylem dysfunction.