Leishmanial excreted factors (EFs): purification by affinity chromatography

Leishmania species grown in culture excrete a polyanionic, carbohydrate-rich factor (EF) which binds to antibodies produced in rabbits against the parent Leishmania species. EF, previously purified by physical and chemical methods, was purified by affinity chromatography on a Ricinus lectin column. The purified samples were characterised and analysed. The results show a notable proportion of galactose in EF and clarify the reasons for its polyanionic properties. Heterogenicity of EF is demonstrated and discussed.     

Trypanosoma lewisi: Immunohematopoietic interrelationships of the infection in normal,hypoxic, and rebound animals

A study has been made of erythropoietic and immune responses in rats subjected to a simulated altitude of 17,000 ft and infected with Trypanosoma lewisi. Observations were also made during the period of rebound (posthypoxic polycythaemia). The erythropoietic response was evaluated on the basis of reticulocyte and hematocrit values in peripheral blood, and the immune response on the basis of the level of parasitemia. When hypoxia and infection were combined, the infection was heavier than in the nonhypoxic animal, as judged by the level of the parasitemia, whereas the erythropoietic response to hypoxia was less intense. Rebound animals, on the other hand, dealt with the infection more effectively, as indicated by the lower level of parasitemia, and the more rapid disappearance of parasites from the blood.     

Cellular changes in the bone marrow of Plasmodium berghei-infected mice: II. Blast transformation and phagocytosis

The present work is concerned with early cellular changes occurring during a malaria infection. Blast transformation by lymphoid cells and phagocytosis by adherent cells from the bone marrow was performed, using immune and nonimmune Balb/c mice. Nonadherent bone marrow cells from immune mice show an increased specific lymphoblast transformation. This increase was not observed during a lethal infection (PI). Adherent bone marrow cells were assayed for phagocytosis of parasitized (PE) or normal erythrocytes (NE). Cells from immune mice show an increase in phagocytosis of PE and NE. Cells from PI mice showed a decreased phagocytosis throughout the infection, beginning at Day 1 after challenge.     

Leishmania spp.: effect of inhibitors on growth and on polyamine and macromolecular syntheses

Ethidium bromide, pentamidine isethionate, and MGBG [methylglyoxal-bis (guanylhydrazone)] inhibited the uptake of radioactive putrescine by leishmanial (Leishmania spp.; Leishmania tropica major; Leishmania mexicana; Leishmania donovani) promastigotes and interfered with their polyamine synthesis. Inhibition was apparent as early as 1 hr after adding these drugs to the parasites at growth-inhibiting concentrations. Ethidium bromide also inhibited the incorporation of radioactive uracil into leishmanial RNA at growth-inhibiting concentrations, while DNA synthesis was inhibited by ethidium bromide at high concentrations after a lag period. MGBG inhibited the synthesis of leishmanial DNA and RNA at growth-inhibiting concentrations.