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401.
Pectin methylesterase was the only pectic enzyme detectablein extracts from rind of sound or Penicillium digitatum-infectedoranges. No pectic enzymes were detected in juice from soundor infected fruit. Extracts from infected rind, and juice frominfected fruit, had macerating activity. Chromatographic analysesof rind extracts, and juice from infected fruit, showed galacturonicacid as a possible product of the degradation of pectic substances.Orange juice contained a thermo labile inhibitor of pectic chain-splitting,and macerating enzymes. 相似文献
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YANPING P. CHEN JAY D. EVANS CHARLES MURPHY ROBIN GUTELL MICHAEL ZUKER DAWN GUNDENSEN-RINDAL JEFF S. PETTIS 《The Journal of eukaryotic microbiology》2009,56(2):142-147
ABSTRACT. Nosema ceranae, a microsporidian parasite originally described from Apis cerana, has been found to infect Apis melllifera and is highly pathogenic to its new host. In the present study, data on the ultrastructure of N. ceranae, presence of N. ceranae-specific nucleic acid in host tissues, and phylogenetic relationships with other microsporidia species are described. The ultrastructural features indicate that N. ceranae possesses all of the characteristics of the genus Nosema. Spores of N. ceranae measured approximately 4.4 × 2.2 μm on fresh smears. The number of coils of the polar filament inside spores was 18–21. Polymerase chain reaction (PCR) signals specific for N. ceranae were detected not only in the primary infection site, the midgut, but also in the tissues of hypopharyngeal glands, salivary glands, Malpighian tubules, and fat body. The detection rate and intensity of PCR signals in the fat body were relatively low compared with other examined tissues. Maximum parsimony analysis of the small subunit rRNA gene sequences showed that N. ceranae appeared to be more closely related to the wasp parasite, Nosema vespula, than to N. apis, a parasite infecting the same host. 相似文献
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The distributions of 50 species of termites across five habitat types in Kakadu National Park are described. Open forests are richest in species and monsoon forests are species-poor. The greatest diversity of termites is associated with infertile soils and is probably related to the enhanced role of termites in these nutrientimpoverished sites. Only the richness of livewood feeders is associated with disturbance in the form of water buffalo impact. Few relationships with physical characteristics of the soil were apparent. Comparisons between continents suggest that lower termites are richer in Australia than on other continents. There are fewer species of soil-feeding termites, but only two of the four subfamilies of the higher termites (Termitidae) are present in Australia. There appears to be a complementary distribution of areas of high diversity of termites and native herbivorous mammals. This may be due to the ability of termites and other invertebrate groups to exploit low fertility systems and has profound implications for the size structure of the vertebrate community. 相似文献
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Root tips (1.0 cm) were excised, transferred to culture medium,sampled at intervals, embedded in paraffin and sectioned. Themitotic index in each primary meristem in the 2.0 mm tips wasreduced immediately after excision. After 812 h mitosisresumed. The position of mitotic figures in the protoderm, groundmeristem, procambium, and root cap was determined. The greatestnumber of mitotic figures occurred in the procambium, and uponexcision the smallest percent reduction also occurred in thisregion. The ground meristem showed the greatest reduction inmitotic figures. After excision, the relative contribution tothe total number of mitotic figures increased in the procambiumtissue and decreased in the ground meristem. Cell division inall primary meristems of the root tip is reduced after tip excision,but the ground meristem (cortical region) is the primary targettissue. 相似文献
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