A new dwarf boa (Serpentes, Booidea, 'Tropidophiidae') from the Early Oligocene of Belgium: a case of the isolation of Western European snake faunas

Falseryx neervelpensis sp. nov. (Booid-grade, 'Tropidophiidae') from the earliest Oligocene (MP 21) of Belgium is described on the basis of vertebrae coming from all major portions of the vertebral column. In its peculiar caudal osteology, the snake approaches the unique morphological pattern characteristic of the living Neotropical Tropidophiinae. This is the first time such a complete and informative vertebral column of a dwarf boa has been described. The genus Falseryx was absent from Western Europe in younger parts of the Oligocene, but reappeared at the end of the Early Miocene. This dispersal pattern provides additional evidence that in most phases of the Oligocene and Early Miocene Western European snake faunas were effectively isolated from possible influences from the East.  © 2008 The Linnean Society of London, Zoological Journal of the Linnean Society , 2008, 152 , 393–406.     

Isolation and characterization of nitrate reductase deficient mutants of the ectomycorrhizal fungus Hebeloma cylindrosporum

To clarify the role of the fungal nitrate assimilation pathway in nitrate reduction by mycorrhizal plants, nitrate reductase (NR)-deficient (NR⁻) mutants of the ectomycorrhizal basidiomycete Hebeloma cylindrosporum Romagnesi have been selected. These mutants were produced by u.v. mutagenesis on protoplasts originating from homokaryotic mycelia belonging to complementary mating types of this heterothallic tetrapolar species. Chlorate-resistant mutants were first selected in the presence of different nitrogen (N) sources in the culture medium. Among 1495 chlorate resistant mycelia, 30 failed to grow on nitrate and lacked a detectable NR activity. Growth tests on different N sources suggested that the NR activity of all the different mutants is specifically impaired as a result of mutations in either the gene coding for NR apoprotein or genes controlling the synthesis of the molybdenum cofactor. Furthermore, restoration of NR activity in some of the dikaryons obtained after crosses between the different mutant mycelia suggested that not all the selected mutations mapped in the same gene. Utilization of N on a NH₄¹⁵NO₃ medium was studied for two mutant strains and their corresponding wild-type homokaryons. None of the mutants could use nitrate whereas ¹⁵N enrichment values indicated that 13–27% of N present in 13-d-old wild-type mycelia originated from nitrate. Apparently, the mutant mycelia do not compensate their inability to use nitrate by a more efficient use of ammonium. These different NR mutants still form mycorrhizas with the habitual host plant, Pinus pinaster (Ait.), making them suitable for study of the contribution of the fungal nitrate assimilation pathway to nitrate assimilation by mycorrhizal plants.     

ORGANIZATION AND REPLICATION ACTIVITY OF THE MITOCHONDRIAL MASS OF OOGONIA AND PREVITELLOGENIC OOCYTES IN XENOPUS LAEVIS

The origin of the mitochondrial mass, previously well characterized in Xenupus diplotene oocytes, has been traced up to oogonia by means of electron microscopy. A polarized organization of the oogonia and of the oocytes of the succeeding stages was observed. The mitochondrial cloud was found to be built up in the centriolar region near the site where the chromosomes will be implanted along the nuclear envelope at the "bouquet" stage. Autoradiographic studies of thymidine incorporation into mitochondrial DNA suggest that mitochondrial DNA synthesis is active throughout this early period of oogenesis.     

Systematics of the extinct reed warblers Acrocephalus of the Society Islands of eastern Polynesia

In the Society archipelago (French Polynesia), Acrocephalus reed warblers are known only from four islands: Tahiti, Mo'orea, Huahine and Raiatea. All populations are now extinct except on Tahiti. Our knowledge of these birds is based on a small number of specimens preserved in museums, collected mostly during the 19th century. We present here a review of the past and present distribution, habitat and threats to the Society Islands reed warblers, including details on the specimens in museum collections. We compare the external morphology of the different populations, and use samples from museum specimens to propose a molecular phylogeny of all taxa based on partial cytochrome b gene sequences. The genetic data do not support the monophyly of the Society Islands reed warblers, which probably derived from three different lineages, found in Tahiti, Mo'orea and in the cluster Raiatea–Huahine. We outline the taxonomic consequences of this phylogeny. Our results support the hypothesis that evolutionary pattern, not distance between islands, shaped the long-distance colonization of oceanic islands by reed warblers.     

Comparison between Ageing of Slices and Ethylene or Rindite Treatments of Whole Potato Tubers: Studies on Microsomal and Mitochondria1 Enzymatic Activities

When potato tubers (Solanum tuberosum L.) are treated with Rindite(ethylene chlorohydrin, ethylene dichloride, carbon tetrachloride,7/3/1, v/v/v) for 2 d, and then stored at 20°C, importantchanges in enzymatic activities are observed in mitochondrialas well as in microsomal fractions isolated from these tubers.In mitochondria isolated from Rindite-treated tubers, the oxidationofsuccinate was more active and more resistant to cyanide thanin mitochondria from untreated tubers. The functioning of acyanide-resistant, SHAM-sensitive oxidative activity was similarto that observed after an ageing treatment of tuber slices oran ethylene treatment of whole tubers. However, in the lattercase, changes in oxidative properties or cyanide-resistancewere less important. Moreover, all the observed changes disappearedafter 4 d of storage. The NADH-femcyanide reductase and NADHcytochromec reductase activities of microsomal fractions isolated fromRindite-treated tubers increased after 1 d or 4 d of storage,relative to the activities of untreated tubers. Moreover, theoleatedesaturase activity, which could not be detccted in microsomesprepared from untreated tubers, was high in microsomes fromtreated tubers stored for 1 d and 4 d, and then the activitydecreased. The induction of an oleatedesaturase activity byRindite treatment can be compared to the effect of the ageingtreatment on tuber slices. The enhancement of oleatedesaturaseactivity was linked to an increase in cytochrome b5content ofmicrosomes of treated tubers. However, all these changes werenot observed when whole tubers were treated with ethylene alone. Key words: Rindite, Mitochondria, Microsomes, Solanum tuberosum     

Changes of DNA Ligases in Chick Neural Retina as a Function of Age

In the course of chick neural retina development, several forms of DNA ligase have been found. During embryonic life the major DNA ligase activity that is found at seven days is form I (8.2 S) which gradually decreases and disappears by 14 days after incubation, whereas form II (6.2 S) increases to reach a maximum at the time of hatching. Form II then decreases reaching a constant level by Day 7 and from that time new slow sedimenting forms also appear (forms III and IV). Form III (2 S) is first detectable at seven days and increases up to 90 days, whereas form IV (3 S) is the only form detected in the 17- and 18-month-old and also in the 5-year-old birds. These four forms display different elution patterns on phosphocellulose column chromatography. They also differ in their thermal stability and sensitivity towards N-ethylmaleimide.     

A giant Upper Jurassic turtle revealed by its trackways

Exceptional fossilization of large tetrapod swimming traces occurs in the Cerin Lagerstätte (Jura Mountains, France). These trackways are imprinted in Jurassic (Late Kimmeridgian) lagoonal fine-grained limestones and are attributed to giant turtles, which swam with a simultaneous movement of their forelimbs like the modern ones. These turtles swam in very shallow waters close to land, perhaps near a nesting area. As a major consequence, these new ichnologic data place the origin of true large marine turtles during the Jurassic period and not during the Cretaceous period as previously considered on the basis of skeletal remains.     

Are neopolyploids a likely route for a transgene walk to the wild? The Aegilops ovata × Triticum turgidum durum case

Spontaneous hybridization between durum wheat ( Triticum turgidum durum ) and Aegilops ovata is regularly observed in nature. The frequency of spontaneous amphiploidy in sympatric populations was estimated at 10⁻⁶ (direct in situ observations and germinated seed collected from A. ovata plants). In nursery conditions some genotype combinations gave frequencies that were much higher at 10⁻³. Genomic in situ hybridization revealed that fertile amphiploids had arisen through unreduced gametes, and that some of them carried wheat −  A. ovata recombinant chromosomes. The frequency of production of unreduced gametes is probably genetically inherited. Amphiploids provide a route for gene flow, including that of transgenes, to the wild. Gene flow could potentially be minimized through the choice of wheat cultivars that produce a low frequency of unreduced gametes.  © 2004 The Linnean Society of London, Biological Journal of the Linnean Society , 2004, 82 , 503–510.     

Les voiles microbiens. Leur contribution à la fossilisation des organismes au corps mou

Dans certains gisements fossilifères, la conservation des organismes au corps mou est imputable à la prolifération de voiles microbiens. II en est ainsi des shales du Grès à Voltzia (Trias inférieur) où des méduses, des annèlides et diverses pontes sont fossilisés dans des lamines grises, d'épaisseur inframillimétrique, dont la nature bactérienne est démontrée. En recouvrant rapidement les cadavres, les voiles microbiens les protégeaient contre les animaux saprophages et les actions mécaniques. En měme temps, ils créaient un milieu clos, appauvri en oxygène, qui inhibait la décomposition de la matière organique. □ Fossilisation, voiles microbiens, cyanobactéries, Buntsandstein.
Preservation of soft-bodied organisms in some fossiliferous localities is attributable to spreading of microbial veils. This is the case of the shales of the Grès à Voltzia (Lower Triassic), where fossil medusae, worms and clutches occur in grey, less than a millimeter thick laminae, the bacterial origin of which is demonstrated. Covering the bodies rapidly, the microbial veils protected them from scavengers and physical injuries. They created enclosed spaces, with poor oxygen content, inhibiting the decay of organic matter. □ Fossilization, microbial veils, cyanobacteria, Buntsandstein.