全文获取类型
收费全文 | 12651篇 |
免费 | 1400篇 |
国内免费 | 243篇 |
专业分类
14294篇 |
出版年
2021年 | 26篇 |
2020年 | 30篇 |
2015年 | 26篇 |
2014年 | 51篇 |
2012年 | 1152篇 |
2011年 | 1354篇 |
2010年 | 202篇 |
2009年 | 89篇 |
2008年 | 1100篇 |
2007年 | 1166篇 |
2006年 | 1094篇 |
2005年 | 1043篇 |
2004年 | 1013篇 |
2003年 | 979篇 |
2002年 | 856篇 |
2001年 | 689篇 |
2000年 | 911篇 |
1999年 | 353篇 |
1998年 | 75篇 |
1997年 | 71篇 |
1996年 | 71篇 |
1995年 | 52篇 |
1994年 | 66篇 |
1993年 | 53篇 |
1992年 | 59篇 |
1991年 | 67篇 |
1990年 | 64篇 |
1989年 | 52篇 |
1988年 | 36篇 |
1987年 | 42篇 |
1986年 | 41篇 |
1985年 | 33篇 |
1984年 | 56篇 |
1983年 | 27篇 |
1982年 | 50篇 |
1981年 | 28篇 |
1980年 | 31篇 |
1979年 | 27篇 |
1959年 | 44篇 |
1958年 | 89篇 |
1957年 | 74篇 |
1956年 | 80篇 |
1955年 | 66篇 |
1954年 | 69篇 |
1953年 | 54篇 |
1952年 | 48篇 |
1951年 | 44篇 |
1950年 | 34篇 |
1949年 | 42篇 |
1948年 | 33篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
981.
The type III secretion system (T3SS) is a specialized apparatus evolved by Gram-negative bacteria to deliver effector proteins into host cells, thus facilitating the establishment of an infection. Effector translocation across the target cell plasma membrane is believed to occur via pores formed by at least two secreted translocator proteins, the functions of which are dependent upon customized class II T3SS chaperones. Recently, three internal tetratricopeptide repeats (TPRs) were identified in this class of chaperones. Here, defined mutagenesis of the class II chaperone PcrH of Pseudomonas aeruginosa revealed these TPRs to be essential for chaperone activity towards the translocator proteins PopB and PopD and subsequently for the translocation of exoenzymes into host cells. 相似文献
982.
983.
984.
985.
The first discernible intermediate when fully reduced cytochrome c oxidase reacts with O2 is a dioxygen adduct (compound A) of the binuclear heme iron-copper center. The subsequent decay of compound A is associated with transfer of an electron from the low-spin heme a to this center. This reaction eventually produces the ferryl state (F) of this center, but whether an intermediate state may be observed between A and F has been the subject of some controversy. Here we show, using both optical and EPR spectroscopy, that such an intermediate (P(R)) indeed exists and that it exhibits spectroscopic properties quite distinct from F. The optical spectrum of P(R) is similar or identical to the spectrum of the P(M) intermediate that is formed after compound A when two-electron-reduced enzyme reacts with O2. An unusual EPR spectrum with features of a CuB(II) ion that interacts magnetically with a nearby paramagnet [cf. Hansson, O., Karlsson, B., Aasa, R., V?nng?rd, T., and Malmstr?m, B.G (1982) EMBO J. 1, 1295-1297; Blair, D. F., Witt, S. N., and Chan, S. I. (1985) J. Am. Chem. Soc. 107, 7389-7399] can be uniquely assigned to the P(R) intermediate, not being found in either the P(M) or F intermediate. The binuclear center in the P(R) state may be assigned as having an Fe(a3)(IV)=O CuB(II) structure, as in both the P(M) and F states. The spectroscopic differences between these three intermediates are evaluated. The P(R) state has a key role as an initiator of proton translocation by the enzyme, and the thermodynamic and electrostatic bases for this are discussed. 相似文献
986.
987.
Isolation and characterization of senescence-induced cDNAs encoding deoxyhypusine synthase and eucaryotic translation initiation factor 5A from tomato 总被引:10,自引:0,他引:10
Full-length cDNA clones encoding deoxyhypusine synthase (DHS) and eucaryotic initiation factor 5A (eIF-5A) have been isolated from a cDNA expression library prepared from tomato leaves (Lycopersicon esculentum, cv. Match) exposed to environmental stress. DHS mediates the first of two enzymatic reactions that activate eIF-5A by converting a conserved lysine to the unusual amino acid, deoxyhypusine. Recombinant protein obtained by expressing tomato DHS cDNA in Escherichia coli proved capable of carrying out the deoxyhypusine synthase reaction in vitro in the presence of eIF-5A. Of particular interest is the finding that DHS mRNA and eIF-5A mRNA show a parallel increase in abundance in senescing tomato flowers, senescing tomato fruit, and environmentally stressed tomato leaves exhibiting programmed cell death. Western blot analyses indicated that DHS protein also increases at the onset of senescence. It is apparent from previous studies with yeast and mammalian cells that hypusine-modified eIF-5A facilitates the translation of a subset of mRNAs mediating cell division. The present study provides evidence for senescence-induced DHS and eIF-5A in tomato tissues that may facilitate the translation of mRNA species required for programmed cell death. 相似文献
988.
989.
990.
Baird CL Gordon MS Andrenyak DM Marecek JF Lindsley JE 《The Journal of biological chemistry》2001,276(30):27893-27898
DNA topoisomerase II catalyzes the transport of one DNA duplex through a transient break in a second duplex using a complex ATP hydrolysis mechanism. Two key rates in the ATPase mechanism, ATP resynthesis and phosphate release, were investigated using 18O exchange and stopped-flow phosphate release experiments, respectively. The 18O exchange results showed that the rate of ATP resynthesis on the topoisomerase II active site was slow compared with the rate of phosphate release. When topoisomerase II was bound to DNA, phosphate was released slowly, with a lag. Since each of the preceding steps is known to occur rapidly, phosphate release is apparently a rate-determining step. The length of the lag phase was unaffected by etoposide, indicating that inhibiting DNA religation inhibits the ATPase reaction cycle at some step following phosphate release. By combining the 18O exchange and phosphate release results, the rate constant for ATP resynthesis can be calculated as approximately 0.5 s(-1). These data support the mechanism of sequential hydrolysis of two ATP by DNA topoisomerase II. 相似文献