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排序方式: 共有254条查询结果,搜索用时 15 毫秒
111.
Auvinen HE Romijn JA Biermasz NR Havekes LM Smit JW Rensen PC Pereira AM 《Hormones et métabolisme》2011,43(13):899-906
Hypothalamus-pituitary-adrenal-axis activity is suggested to be involved in the pathophysiology of the metabolic syndrome. In diet-induced obesity mouse models, features of the metabolic syndrome are induced by feeding high fat diet. However, the models reveal conflicting results with respect to the hypothalamus-pituitary-adrenal-axis activation. The aim of this review was to assess the effects of high fat feeding on the activity of the hypothalamus-pituitary-adrenal-axis in mice. PubMed, EMBASE, Web of Science, the Cochrane database, and Science Direct were electronically searched and reviewed by 2 individual researchers. We included only original mouse studies reporting parameters of the hypothalamus-pituitary-adrenal-axis after high fat feeding, and at least 1 basal corticosterone level with a proper control group. Studies with adrenalectomized mice, transgenic animals only, high fat diet for less than 2 weeks, or other interventions besides high fat diet, were excluded. 20 studies were included. The hypothalamus-pituitary-adrenal-axis evaluation was the primary research question in only 5 studies. Plasma corticosterone levels were unchanged in 40%, elevated in 30%, and decreased in 20% of the studies. The effects in the peripheral tissues and the central nervous system were also inconsistent. However, major differences were found between mouse strains, experimental conditions, and the content and duration of the diets. This systematic review demonstrates that the effects of high fat feeding on the basal activity of the hypothalamus-pituitary-adrenal-axis in mice are limited and inconclusive. Differences in experimental conditions hamper comparisons and accentuate the need for standardized evaluations to discern the effects of diet-induced obesity on the hypothalamus-pituitary-adrenal-axis. 相似文献
112.
van Eijk M Bruinsma L Hartshorn KL White MR Rynkiewicz MJ Seaton BA Hemrika W Romijn RA van Balkom BW Haagsman HP 《The Journal of biological chemistry》2011,286(23):20137-20151
Porcine surfactant protein D (pSP-D) displays distinctively strong, broad-range inhibitory activity against influenza A virus (IAV). N-Linked glycosylation of the carbohydrate recognition domain (CRD) of pSP-D contributes to the high affinity of this collectin for IAV. To investigate the role of the N-linked glycan further, HEK293E protein expression was used to produce recombinant pSP-D (RpSP-D) that has similar structural and antiviral properties as NpSP-D. We introduced an additional N-linked glycan in the CRD of RpSP-D but this modification did not alter the antiviral activity. Human SP-D is unglycosylated in its CRD and less active against IAV compared with pSP-D. In an attempt to modify its antiviral properties, several recombinant human SP-D (RhSP-D) mutants were constructed with N-linked glycans introduced at various locations within its CRD. To retain lectin activity, necessary for the primary interactions between SP-D and IAV, N-linked glycosylation of RhSP-D was shown to be restricted to the corresponding position in the CRD of either pSP-D or surfactant protein A (SP-A). These N-glycosylated RhSP-D mutants, however, did not show increased neutralization activity against IAV. By developing RhSP-D mutants that also have the pSP-D-specific Ser-Gly-Ala loop inserted in the CRD, we could demonstrate that the N-linked glycan-mediated interactions between pSP-D and IAV involves additional structural prerequisites of the pSP-D CRD. Ultimately, these studies will help to develop highly effective SP-D-based therapeutic and prophylactic drugs against IAV. 相似文献
113.
JC Barbero-Alvarez JV Subiela J Granda-Vera C Castagna M Gómez J Del Coso 《Biology of sport / Institute of Sport》2015,32(4):339-344
Despite its growing popularity, few studies have investigated specific physiological demands for elite female futsal. The aim of this study was to determine aerobic fitness in elite female futsal players using laboratory and field testing. Fourteen female futsal players from the Venezuelan National team (age =21.2±4.0 years; body mass =58.6±5.6 kg; height =161±5.0 cm) performed a progressive maximal treadmill test under laboratory conditions. Players also performed a progressive intermittent futsal-specific field test for endurance, the Futsal Intermittent Endurance Test (FIET), until volitional fatigue. Outcome variables were exercise heart rate (HR), VO2, post-exercise blood lactate concentrations ([La]b) and running speeds (km · h-1). During the treadmill test, VO2max, maximal aerobic speed (MAS), HR and peak [La]b were 45.3±5.6 ml · kg-1 · min-1, 12.5±1.77 km · h-1, 197±8 beats · min-1 and 11.3±1.4 mmol · l-1, respectively. The FIET total distance, peak running velocity, peak HR and [La]b were 1125.0±121.0 m, 15.2±0.5 km · h-1, 199±8 beats · min-1 and 12.5±2.2 mmol · l-1, respectively. The FIET distance and peak speed were strongly associated (r= 0.85-87, p < 0.0001) with VO2max and MAS, respectively. Peak HR and [La]b were not significantly different between tests. Elite female futsal players possess moderate aerobic fitness. Furthermore, the FIET can be considered as a valid field test to determine aerobic fitness in elite level female futsal players. 相似文献
114.
Loss of phylogenetic information in chorion gene families of Bombyx mori gene conversion 总被引:1,自引:0,他引:1
Regier JC; Weigmann BM; Leclerc RF; Friedlander TP 《Molecular biology and evolution》1994,11(1):72-87
The silkmoth chorion has provided a stimulating model for the study of
evolution and developmental regulation of gene families. Previous attempts
at inferring relationships among chorion sequences have been based on
pairwise comparisons of overall similarity, a potentially problematic
approach. To remedy this, we identified the alignable regions of low
sequence variability and then analyzed this restricted database by
parsimony and neighbor-joining methods. At the deepest level, the chorion
sequence tree is split into two branches, called "alpha" and "beta." Within
each branch, early- and late-expressing genes each constitute monophyletic
groups, while the situation with middle-expressing genes remains uncertain.
The HcB gene family appears to be the most basal beta-branch group, but
this conclusion is qualified because the effect of gene conversion on
branching order is unknown. Previous studies by Eickbush and colleagues
have strongly suggested that ErA, HcA, and HcB families undergo gene
conversion within a gene family, whereas the ErB family does not. The
occurrence of conversion correlates with a particular tree structure;
namely, branch lengths are much greater at the base of the family than at
higher internodes and terminal branches. These observations raise the
possibility that chorion gene families are defined by gene conversion
events (reticulate evolution) rather than by descent with modification
(synapomorphy).
相似文献
115.
Andrea E. Bochem Adriaan G. Holleboom Johannes A. Romijn Menno Hoekstra Geesje M. Dallinga Mahdi M. Motazacker G. Kees Hovingh Jan A. Kuivenhoven Erik S. G. Stroes 《PloS one》2014,9(5)
Introduction
Adrenal steroidogenesis is essential for human survival and depends on the availability of the precursor cholesterol. Male subjects with low plasma levels of high density lipoprotein (HDL) cholesterol are characterized by decreased adrenal function. Whether this is also the case in female subjects with low plasma HDL-C levels is unresolved to date.Findings
15 female ATP binding cassette transporter AI (ABCAI) and 14 female lecithin-cholesterol acyltransferase (LCAT) were included in the study. HDL-C levels were 38% and 41% lower in ABCA1 and LCAT mutation carriers compared to controls, respectively. Urinary steroid excretion of 17-ketogenic steroids or 17-hydroxy corticosteroids did not differ between 15 female ABCA1 mutation carriers (p = 0.27 vs 0.30 respectively) and 30 matched normolipidemic controls or between 14 female LCAT mutation carriers and 28 matched normolipidemic controls (p = 0.10 and 0.14, respectively). Cosyntropin testing in an unselected subgroup of 8 ABCA1 mutation carriers and 3 LCAT mutation carriers did not reveal differences between carriers and controls.Conclusion
Adrenal function in females with molecularly defined low HDL-C levels is not different from controls. The discrepancy with the finding of impaired steroidogenesis in males with molecularly defined low HDL-C levels underscores the importance of gender specific analyses in cholesterol-related research. 相似文献116.
Tan JC Miller BA Tan A Patel JJ Cheeseman IH Anderson TJ Manske M Maslen G Kwiatkowski DP Ferdig MT 《Genome biology》2011,12(4):R35
We present an optimized probe design for copy number variation (CNV) and SNP genotyping in the Plasmodium falciparum genome. We demonstrate that variable length and isothermal probes are superior to static length probes. We show that sample
preparation and hybridization conditions mitigate the effects of host DNA contamination in field samples. The microarray and
workflow presented can be used to identify CNVs and SNPs with 95% accuracy in a single hybridization, in field samples containing
up to 92% human DNA contamination. 相似文献
117.
Edwin T. Parlevliet Yanan Wang Janine J. Geerling Janny P. Schr?der-Van der Elst Kristen Picha Karyn O'Neil Vedrana Stojanovic-Susulic Tatiana Ort Louis M. Havekes Johannes A. Romijn Hanno Pijl Patrick C. N. Rensen 《PloS one》2012,7(11)
Objective
In addition to improve glucose intolerance, recent studies suggest that glucagon-like peptide-1 (GLP-1) receptor agonism also decreases triglyceride (TG) levels. The aim of this study was to evaluate the effect of GLP-1 receptor agonism on very-low-density lipoprotein (VLDL)-TG production and liver TG metabolism.Experimental Approach
The GLP-1 peptide analogues CNTO3649 and exendin-4 were continuously administered subcutaneously to high fat diet-fed APOE*3-Leiden transgenic mice. After 4 weeks, hepatic VLDL production, lipid content, and expression profiles of selected genes involved in lipid metabolism were determined.Results
CNTO3649 and exendin-4 reduced fasting plasma glucose (up to −30% and −28% respectively) and insulin (−43% and −65% respectively). In addition, these agents reduced VLDL-TG production (−36% and −54% respectively) and VLDL-apoB production (−36% and −43% respectively), indicating reduced production of VLDL particles rather than reduced lipidation of apoB. Moreover, they markedly decreased hepatic content of TG (−39% and −55% respectively), cholesterol (−30% and −55% respectively), and phospholipids (−23% and −36% respectively), accompanied by down-regulation of expression of genes involved in hepatic lipogenesis (Srebp-1c, Fasn, Dgat1) and apoB synthesis (Apob).Conclusion
GLP-1 receptor agonism reduces VLDL production and hepatic steatosis in addition to an improvement of glycemic control. These data suggest that GLP-receptor agonists could reduce hepatic steatosis and ameliorate dyslipidemia in patients with type 2 diabetes mellitus. 相似文献118.
Snel M Jonker JT Hammer S Kerpershoek G Lamb HJ Meinders AE Pijl H de Roos A Romijn JA Smit JW Jazet IM 《Obesity (Silver Spring, Md.)》2012,20(8):1572-1576
Pericardial fat accumulation has been associated with an increased cardiovascular risk. A very low calorie diet (VLCD) improves the cardiovascular risk profile in patients with type 2 diabetes mellitus (T2DM), by improving the metabolic profile, heart function, and triglyceride (TG) stores in (non)adipose tissues. However, long-term effects of a VLCD on pericardial fat volume and tissue-specific TG accumulation have not been documented. The aim of this study was therefore to assess the effects of a 16-week VLCD and of subsequent 14 months follow-up on a regular diet on pericardial fat in relation to other TG stores in obese T2DM patients. We included 14 obese patients with insulin-treated T2DM (mean ± s.e.m.: age 53 ± 2 years; BMI 35 ± 1 kg/m(2)). Pericardial fat and other (non)adipose TG stores were measured using magnetic resonance (MR) imaging and proton spectroscopy before and after a 16-week VLCD and after a 14-month follow-up without dietary interventions. A 16-week VLCD reduced body weight, pericardial fat, hepatic TG content, visceral and subcutaneous abdominal fat volumes to 78, 83, 16, 40, and 53% of baseline values respectively, (all P < 0.05). After an additional 14 months of follow-up on a regular diet, the reduction in pericardial fat volume sustained, despite a substantial regain in body weight, visceral abdominal fat, and hepatic TG content (respectively 90, 83 and 73% of baseline values). In conclusion, VLCD-induced weight loss in obese T2DM patients is accompanied by a substantial decrease in pericardial fat volume, which is sustained even after subsequent weight regain. 相似文献
119.
The Glutaraldehyde test (GT), a rapid and inexpensive test, has been utilized empirically for many years in bovine practice
for diagnosing inflammatory diseases. GT is used primarily to demonstrate increased serum concentrations of fibrinogen and
globulin. Glutaraldehyde binds with free amino groups in fibrinogen and immunoglobulin to create a clot in a first degree
chemical reaction. The clotting time of the GT estimates the content of proteins produced in response to inflammation. The
applicability of GT for diagnosing inflammation in the horse has never been investigated. The objective of this study was
to determine the ability of GT to distinguish between acute and chronic inflammatory disease in horses. Thirty-seven horses
with suspected inflammatory diseases were evaluated using the GT, history, complete clinical examination and routine blood
analysis. GT-times, laboratory results and clinical outcome were compared statistically. Horses that were determined to be
acutely affected (based on history, clinical examination and routine blood analysis) tended to have a negative GT (75%). Results
of the GT did not correlate with blood fibrinogen concentration. Positive GT also predicted a fatal outcome in 69% of the
clinical cases. The results of this trial indicate that GT can be a useful screening test to distinguish between acute and
chronic inflammatory disease in horses. 相似文献
120.