排序方式: 共有89条查询结果,搜索用时 234 毫秒
41.
42.
Keiji Uehara Toshimasa Harumoto Asana Makino Yasuo Koda Junko Iwano Yasuhiro Suzuki Mari Tanigawa Hiroto Iwai Kana Asano Kana Kurihara Akinori Hamaguchi Hiroshi Kodaira Toshiyuki Atsumi Yoji Yamada Kazuma Tomizuka 《Nucleic acids research》2022,50(9):4840
Extrahepatic delivery of small interfering RNAs (siRNAs) may have applications in the development of novel therapeutic approaches. However, reports on such approaches are limited, and the scarcity of reports concerning the systemically targeted delivery of siRNAs with effective gene silencing activity presents a challenge. We herein report for the first time the targeted delivery of CD206-targetable chemically modified mannose–siRNA (CMM–siRNA) conjugates to macrophages and dendritic cells (DCs). CMM–siRNA exhibited a strong binding ability to CD206 and selectively delivered contents to CD206-expressing macrophages and DCs. Furthermore, the conjugates demonstrated strong gene silencing ability with long-lasting effects and protein downregulation in CD206-expressing cells in vivo. These findings could broaden the use of siRNA technology, provide additional therapeutic opportunities, and establish a basis for further innovative approaches for the targeted delivery of siRNAs to not only macrophages and DCs but also other cell types. 相似文献
43.
Satoshi Shuto Tatsuyoshi Iwano Hideo Inoue Tohru Ueda 《Nucleosides, nucleotides & nucleic acids》2013,32(3):263-273
Abstract Deamination of 1-(3-amino-3-deoxy-β-D-glucopyranosyl)-uracil gave a ring contracted nucleoside, 3′-deoxy-3′-formyluridine as a hemiacetal form, and uracil. Similar treatment of the 2′-deoxyderivative, 1-(3-amino-2,3-dideoxy-β-D-glucopyranosyl)uracil, gave the corresponding 2′,3′-dideoxy-3′-formyluridine in high yield. The 3′-epimerization of the 3′-formyluridine derivative was achieved and after reduction of the formyl groups, 2′,3′-dideoxy-3′(R and S)-hydroxymethyluridine were obtained. 相似文献
44.
Hidetaka Kaya Ryo Nakajima Megumi Iwano Masahiro M. Kanaoka Sachie Kimura Seiji Takeda Tomoko Kawarazaki Eriko Senzaki Yuki Hamamura Tetsuya Higashiyama Seiji Takayama Mitsutomo Abe Kazuyuki Kuchitsu 《The Plant cell》2014,26(3):1069-1080
In flowering plants, pollen germinates on the stigma and pollen tubes grow through
the style to fertilize the ovules. Enzymatic production of reactive oxygen species
(ROS) has been suggested to be involved in
pollen tube tip growth. Here, we characterized the function and regulation of the
NADPH oxidases RbohH and RbohJ (Respiratory burst oxidase homolog H and J) in pollen
tubes in Arabidopsis thaliana. In the rbohH and
rbohJ single mutants, pollen tube tip growth was comparable to
that of the wild type; however, tip growth was severely impaired in the double
mutant. In vivo imaging showed that ROS
accumulation in the pollen tube was impaired in the double mutant. Both RbohH and
RbohJ, which contain Ca2+ binding EF-hand motifs, possessed
Ca2+-induced ROS-producing
activity and localized at the plasma membrane of the pollen tube tip. Point mutations
in the EF-hand motifs impaired Ca2+-induced ROS production and complementation of the double mutant
phenotype. We also showed that a protein phosphatase inhibitor enhanced the
Ca2+-induced ROS-producing
activity of RbohH and RbohJ, suggesting their synergistic activation by protein
phosphorylation and Ca2+. Our results suggest that ROS production by RbohH and RbohJ is essential for proper pollen
tube tip growth, and furthermore, that Ca2+-induced ROS positive feedback regulation is conserved in the polarized
cell growth to shape the long tubular cell. 相似文献
45.
Shinya Fukumoto Kiwamu Hanazono Dah-Renn Fu Yoshifumi Endo Tsuyoshi Kadosawa Hidetomo Iwano Tsuyoshi Uchide 《Biochemical and biophysical research communications》2013
L-type amino acid transporter 1 (LAT1), an isoform of amino acid transport system L, transports branched or aromatic amino acids essential for fundamental cellular activities such as cellular growth, proliferation and maintenance. This amino acid transporter recently has received attention because of its preferential and up-regulated expression in a variety of human tumors in contrast to its limited distribution and low-level expression in normal tissues. In this study, we explored the feasibility of using LAT1 inhibitor as a new therapeutic agent for human malignant melanomas (MM) using canine spontaneous MM as a model for human MM. A comparative study of LAT expression was performed in 48 normal tissues, 25 MM tissues and five cell lines established from MM. The study observed LAT1 mRNA levels from MM tissues and cell lines that were significantly (P < 0.01) higher than in normal tissues. Additionally, MM with distant metastasis showed a higher expression than those without distant metastasis. Functional analysis of LAT1 was performed on one of the five cell lines, CMeC-1. [3H]l-Leucine uptake and cellular growth activities in CMeC-1 were inhibited in a dose-dependent manner by selective LAT1 inhibitors (2-amino-2-norbornane-carboxylic acid, BCH and melphalan, LPM). Inhibitory growth activities of various conventional anti-cancer drugs, including carboplatin, cyclophosphamide, dacarbazine, doxorubicin, mitoxantrone, nimustine, vinblastine and vincristine, were significantly (P < 0.05) enhanced by combination use with BCH or LPM. These findings suggest that LAT1 could be a new therapeutic target for MM. 相似文献
46.
Taizo Iwano 《Primates; journal of primatology》1989,30(2):241-248
A total of nine groups of ruffed lemurs (Varecia variegata variegata), as well as one solitary individual, were observed on Nosy Mangabe, an island located off the eastern coast of Madagascar,
for a total 536 min between November 30 and December 8, 1983. The average group size was 2.8 animals (N=5), and the population density was 175 animals/km2, as estimated from direct observation and location of vocalization. During the same period, nine groups of brown lemurs (Lemur fulvus albifrons) and one solitary individual were observed for a total 320 min. One group of brown lemurs was composed of eight animals (two
adult males and females, one male juvenile, two infants, and one individual of undetermined age and sex). Feeding competition
occurred between the two species, with whichVarecia variegata variegata dominating. 相似文献
47.
Kozo Tsuchida Takeru Yokoyama Takashi Sakudoh Chihiro Katagiri Shuichiro Tsurumaru Naoko Takada Hirofumi Fujimoto Rolf Ziegler Hidetoshi Iwano Kunikatsu Hamano Toshinobu Yaginuma 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》2010,155(4):363-370
We examined the expression of apolipophorin-III (apoLp-III) during embryonic development of the silkworm Bombyx mori. ApoLp-III mRNA was first expressed 24 h after oviposition, which corresponds to the time of germ band formation. The amount of apoLp-III in the eggs increased from day 2, peaked on day 4, and then gradually decreased until hatching (on day 9.5). ApoLp-III was apparently synthesized during early embryogenesis, as radioactive amino acids were incorporated into newly synthesized apoLp-III in three-day-old eggs. Moreover, radioactive apoLp-III was found only in the embryo and not in the extraembryonic tissue. KBr density gradient ultracentrifugation of egg homogenates showed that apoLp-III was associated with low-density lipophorin (LDLp). These results suggest that LDLp is required for the delivery of lipids for organogenesis during embryogenesis. 相似文献
48.
Interleukin-6 (IL-6) functions as an in vitro autocrine growth factor in renal cell carcinomas 总被引:19,自引:0,他引:19
S Miki M Iwano Y Miki M Yamamoto B Tang K Yokokawa T Sonoda T Hirano T Kishimoto 《FEBS letters》1989,250(2):607-610
Interleukin-6 (IL-6) was found to be a growth factor of renal cell carcinomas Furthermore, renal cell carcinomas freshly isolated from the patients expressed mRNA of IL-6 and secreted biologically active IL-6 under the culture conditions where the tumor cells could grow, but they did not produce IL-6 nor proliferate in the absence of fetal calf serum. The production of IL-6 by the tumor cells was also demonstrated by immunostaining of the IL-6-producing cells utilizing anti-IL-6 antiserum. Moreover, anti-IL-6 antiserum specifically inhibited the in vitro tumor growth. All data indicated that IL-6 functions as an in vitro autocrine growth factor of renal cell carcinomas. 相似文献
49.
Effects of calmodulin antagonists on human ovarian cancer cell proliferation in vitro 总被引:3,自引:0,他引:3
Examined were effects of calmodulin antagonists (W-5 and W-7) on proliferation of two kinds of human cell lines, designated HR and KF, derived from serous cystadecarcinoma of the ovary. Although both W-5 and W-7 inhibited their cell proliferation in vitro, the degree of inhibition was more marked with W-5 rather than with W-7. HR cells had higher sensitivity to cisplatinum than KF cells, while KF cells had higher sensitivity to adriamycin. Combinations of calmodulin antagonists and anti-cancer drugs resulted in adjuvant effects with regard to the inhibition of their cell proliferation in vitro. 相似文献
50.
This study was performed to prove our hypothesis that the metabolite(s) of polycyclic aromatic hydrocarbons (PAHs) caused the activation or phosphorylation of p53 via DNA damage to suppress the liver X receptor (LXR)-mediated signal transductions as a probably more direct mechanism. We found that LXR-mediated trans-activation was inhibited by 3-methylchoranthrene (MC) and doxorubicin (Dox) in HepG2 cells carrying wild-type p53, but not in Hep3B cells possessing mutant p53. The exogenous expression of wild-type p53 suppressed the LXR-mediated trans-activation in Hep3B cells. The expression of mRNA for ATP binding cassette A1 was suppressed by MC and Dox in HepG2 cells. The protein expression of retinoid X receptor (RXR), a partner of LXR to form a heterodimer, was suppressed by MC and Dox in HepG2 cells. 相似文献