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991.
Göker M Held B Lucas S Nolan M Yasawong M Glavina Del Rio T Tice H Cheng JF Bruce D Detter JC Tapia R Han C Goodwin L Pitluck S Liolios K Ivanova N Mavromatis K Mikhailova N Pati A Chen A Palaniappan K Land M Hauser L Chang YJ Jeffries CD Rohde M Sikorski J Pukall R Woyke T Bristow J Eisen JA Markowitz V Hugenholtz P Kyrpides NC Klenk HP Lapidus A 《Standards in genomic sciences》2010,3(1):76-84
Olsenella uli (Olsen et al. 1991) Dewhirst et al. 2001 is the type species of the genus Olsenella, which belongs to the actinobacterial family Coriobacteriaceae. The species is of interest because it is frequently isolated from dental plaque in periodontitis patients and can cause primary endodontic infection. The species is a Gram-positive, non-motile and non-sporulating bacterium. The strain described in this study was isolated from human gingival crevices. This is the first completed sequence of the genus Olsenella and the fifth sequence from a member of the family Coriobacteriaceae. The 2,051,896 bp long genome with its 1,795 protein-coding and 55 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project. 相似文献
992.
Chang YJ Pukall R Saunders E Lapidus A Copeland A Nolan M Glavina Del Rio T Lucas S Chen F Tice H Cheng JF Han C Detter JC Bruce D Goodwin L Pitluck S Mikhailova N Liolios K Pati A Ivanova N Mavromatis K Chen A Palaniappan K Land M Hauser L Jeffries CD Brettin T Rohde M Göker M Bristow J Eisen JA Markowitz V Hugenholtz P Kyrpides NC Klenk HP 《Standards in genomic sciences》2010,3(1):1-14
Acidaminococcus fermentans (Rogosa 1969) is the type species of the genus Acidaminococcus, and is of phylogenetic interest because of its isolated placement in a genomically little characterized region of the Firmicutes. A. fermentans is known for its habitation of the gastrointestinal tract and its ability to oxidize trans-aconitate. Its anaerobic fermentation of glutamate has been intensively studied and will now be complemented by the genomic basis. The strain described in this report is a nonsporulating, nonmotile, Gram-negative coccus, originally isolated from a pig alimentary tract. Here we describe the features of this organism, together with the complete genome sequence, and annotation. This is the first complete genome sequence of a member of the family Acidaminococcaceae, and the 2,329,769 bp long genome with its 2,101 protein-coding and 81 RNA genes is part of the Genomic Encyclopedia of Bacteria and Archaea project. 相似文献
993.
Tindall BJ Sikorski J Lucas S Goltsman E Copeland A Glavina Del Rio T Nolan M Tice H Cheng JF Han C Pitluck S Liolios K Ivanova N Mavromatis K Ovchinnikova G Pati A Fähnrich R Goodwin L Chen A Palaniappan K Land M Hauser L Chang YJ Jeffries CD Rohde M Göker M Woyke T Bristow J Eisen JA Markowitz V Hugenholtz P Kyrpides NC Klenk HP Lapidus A 《Standards in genomic sciences》2010,3(1):26-36
Meiothermus ruber (Loginova et al. 1984) Nobre et al. 1996 is the type species of the genus Meiothermus. This thermophilic genus is of special interest, as its members share relatively low degrees of 16S rRNA gene sequence similarity and constitute a separate evolutionary lineage from members of the genus Thermus, from which they can generally be distinguished by their slightly lower temperature optima. The temperature related split is in accordance with the chemotaxonomic feature of the polar lipids. M. ruber is a representative of the low-temperature group. This is the first completed genome sequence of the genus Meiothermus and only the third genome sequence to be published from a member of the family Thermaceae. The 3,097,457 bp long genome with its 3,052 protein-coding and 53 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project. 相似文献
994.
Saunders E Tindall BJ Fähnrich R Lapidus A Copeland A Del Rio TG Lucas S Chen F Tice H Cheng JF Han C Detter JC Bruce D Goodwin L Chain P Pitluck S Pati A Ivanova N Mavromatis K Chen A Palaniappan K Land M Hauser L Chang YJ Jeffries CD Brettin T Rohde M Göker M Bristow J Eisen JA Markowitz V Hugenholtz P Klenk HP Kyrpides NC 《Standards in genomic sciences》2010,2(1):107-116
Haloterrigena turkmenica (Zvyagintseva and Tarasov 1987) Ventosa et al. 1999, comb. nov. is the type species of the genus Haloterrigena in the euryarchaeal family Halobacteriaceae. It is of phylogenetic interest because of the yet unclear position of the genera Haloterrigena and Natrinema within the Halobacteriaceae, which created some taxonomic problems historically. H. turkmenica, was isolated from sulfate saline soil in Turkmenistan, is a relatively fast growing, chemoorganotrophic, carotenoid-containing, extreme halophile, requiring at least 2 M NaCl for growth. Here we describe the features of this organism, together with the complete genome sequence, and annotation. This is the first complete genome sequence of the genus Haloterrigena, but the eighth genome sequence from a member of the family Halobacteriaceae. The 5,440,782 bp genome (including six plasmids) with its 5,287 protein-coding and 63 RNA genes is part of the Genomic Encyclopedia of Bacteria and Archaea project. 相似文献
995.
Brambilla E Djao OD Daligault H Lapidus A Lucas S Hammon N Nolan M Tice H Cheng JF Han C Tapia R Goodwin L Pitluck S Liolios K Ivanova N Mavromatis K Mikhailova N Pati A Chen A Palaniappan K Land M Hauser L Chang YJ Jeffries CD Rohde M Spring S Sikorski J Göker M Woyke T Bristow J Eisen JA Markowitz V Hugenholtz P Kyrpides NC Klenk HP 《Standards in genomic sciences》2010,2(2):203-211
Segniliparus rotundus Butler 2005 is the type species of the genus Segniliparus, which is currently the only genus in the corynebacterial family Segniliparaceae. This family is of large interest because of a novel late-emerging genus-specific mycolate pattern. The type strain has been isolated from human sputum and is probably an opportunistic pathogen. Here we describe the features of this organism, together with the complete genome sequence and annotation. This is the first completed genome sequence of the family Segniliparaceae. The 3,157,527 bp long genome with its 3,081 protein-coding and 52 RNA genes is part of the Genomic Encyclopedia of Bacteria and Archaea project. 相似文献
996.
Harmon-Smith M Celia L Chertkov O Lapidus A Copeland A Glavina Del Rio T Nolan M Lucas S Tice H Cheng JF Han C Detter JC Bruce D Goodwin L Pitluck S Pati A Liolios K Ivanova N Mavromatis K Mikhailova N Chen A Palaniappan K Land M Hauser L Chang YJ Jeffries CD Brettin T Göker M Beck B Bristow J Eisen JA Markowitz V Hugenholtz P Kyrpides NC Klenk HP Chen F 《Standards in genomic sciences》2010,2(2):220-227
Sebaldella termitidis (Sebald 1962) Collins and Shah 1986, is the only species in the genus Sebaldella within the fusobacterial family 'Leptotrichiaceae'. The sole and type strain of the species was first isolated about 50 years ago from intestinal content of Mediterranean termites. The species is of interest for its very isolated phylogenetic position within the phylum Fusobacteria in the tree of life, with no other species sharing more than 90% 16S rRNA sequence similarity. The 4,486,650 bp long genome with its 4,210 protein-coding and 54 RNA genes is part of the Genomic Encyclopedia of Bacteria and Archaea project. 相似文献
997.
The effects of synthetic polycation polyallylamine (PAA) on adhesion of CHL V-79 RJK fibroblasts and CHL V-79 RJK40 cells resistant to 40 degrees C, and attachment to these cells to polycation immobilized on polystyrene surface were studied. We have also investigated the cytotoxicity of PAA. It was shown that cell adherence to polystyrene plastic coated with PAA was enhanced or decreased in dependence of the PAA concentration used for surface coating and did not depend on heat resistance of investigated cell lines. The effect of PAA on cell adhesion to uncoated polystyrene surface after cell exposure with PAA depended not only on the polycation concentration, but also on the extent of heat resistance of investigated cell lines. Pretreatment of CHL V-79 RJK cells with PAA at the nontoxic concentrations led to inhibition of cell adhesion, and no change in adhesive properties of thermoresistant cells was found under the same conditions. PAA was toxic for CHL V-79 RJK and CHL V-79 RJK40 cells only at concentrations of 100 microg/ml (MTT assay). PAA-induced acute toxicity was accompanied by necrotic-like cell damage. Possible mechanisms of the PAA effect on the behaviour of cells with different structural and metabolic characteristics that are due to the temperature of cell cultivation are discussed. 相似文献
998.
Background
Probing the complex fusion of genetic and environmental interactions, metabolic profiling (or metabolomics/metabonomics), the study of small molecules involved in metabolic reactions, is a rapidly expanding 'omics' field. A major technique for capturing metabolite data is 1H-NMR spectroscopy and this yields highly complex profiles that require sophisticated statistical analysis methods. However, experimental data is difficult to control and expensive to obtain. Thus data simulation is a productive route to aid algorithm development. 相似文献999.
T. E. Fedorova S. Z. Ivanova V. A. Babkin 《Russian Journal of Bioorganic Chemistry》2010,36(7):793-801
This review summarizes the results of research aimed at isolation and structural characterization of flavonoid compounds belonging
to a new class, namely, spirobiflavonoids, from different plant sources. 相似文献
1000.
Larisa M. Skobeltsyna Dmitrii V. Pyshnyi Eugenia M. Ivanova Vadim A. Stepanov Valery P. Puzyrev Grigory M. Dymshits Vladimir N. Kharkov Valentina F. Zarytova 《Molecular biotechnology》2010,45(1):1-8
We propose a novel universal methodology, Short Oligonucleotide Tandem Ligation Assay (SOTLA), for SNP genotyping. SOTLA is
based on using a tandem of short oligonucleotide (TSO) probes consisting of three fragments: the core oligonucleotide and
two flanking oligomers, one of which is immobilized onto a solid support and another one contains the biotin label. TSO is
self-associated on a complementary DNA template, forms the complex containing two nicks, which are efficiently ligated with
DNA ligase giving biotinylated oligonucleotide covalently bound to polymer beads. No ligation of TSO on an imperfect DNA template
bearing the base substitution in the core binding site is occurred. We used SOTLA for the highly selective SNP analysis in
different DNA fragments of human Y chromosome. Comparison of SOTLA results with those of PCR-RFLP and allele-specific PCR
techniques demonstrates that SOTLA ensures the univocal reliable SNP analysis in different PCR fragments varying in length
and base composition. The fundamental difference between SOTLA and well known OLA approaches while using T4 DNA ligase is
that the accuracy of SNP analysis in OLA is ensured only by the specificity of ligase while that in SOTLA is provided by the
specificity of both ligation and hybridization of TSO probes. 相似文献