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981.
Mátrai J Lammens W Jonckheer A Le Roy K Rabijns A Van den Ende W De Maeyer M 《Proteins》2008,71(2):552-564
In the present study, we report on the X-ray crystallographic structure of a GH32 invertase mutant, (i.e., the Arabidopsis thaliana cell-wall invertase 1-E203Q, AtcwINV1-mutant) in complex with sucrose. This structure was solved to reveal the features of sugar binding in the catalytic pocket. However, as demonstrated by the X-ray structure the sugar binding and the catalytic pocket arrangement is significantly altered as compared with what was expected based on previous X-ray structures on GH-J clan enzymes. We performed a series of docking and molecular dynamics simulations on various derivatives of AtcwINV1 to reveal the reasons behind this modified sugar binding. Our results demonstrate that the E203Q mutation introduced into the catalytic pocket triggers conformational changes that alter the wild type substrate binding. In addition, this study also reveals the putative productive sucrose binding modus in the wild type enzyme. 相似文献
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983.
Acosta JC Ferrándiz N Bretones G Torrano V Blanco R Richard C O'Connell B Sedivy J Delgado MD León J 《Molecular and cellular biology》2008,28(24):7286-7295
984.
Summary Transverse sections of leaves of some aluminium-accumulating and nonaccumulating species of the cerrado vegetation of central
Brazil were coloured using aluminon to identify the tissues where aluminium occurs or is deposited. None of the tissues of
the nonaccumulating species showed evidence of high concentrations of Al. All of the aluminium accumulating species showed
high concentrations of Al in all of the elements of the phloem of the midrib and the secondary veins and total absence of
it in the vessel members, xylem fibres and the palisade parenchyma. Walls and contents of the collenchyma of the midrib, epidermal
cells, guard cells of the stomata and spongy parenchyma showed evidence of high concentrations of Al in the accumulating species. 相似文献
985.
Lampa A Ehrenberg AE Vema A Åkerblom E Lindeberg G Danielson UH Karlén A Sandström A 《Bioorganic & medicinal chemistry》2011,19(16):4917-4927
Macrocyclization is a commonly used strategy to preorganize HCV NS3 protease inhibitors in their bioactive conformation. Moreover, macrocyclization generally leads to greater stability and improved pharmacokinetic properties. In HCV NS3 protease inhibitors, it has been shown to be beneficial to include a vinylated phenylglycine in the P2 position in combination with alkenylic P1' substituents. A series of 14-, 15- and 16-membered macrocyclic HCV NS3 protease inhibitors with the linker connecting the P2 phenylglycine and the alkenylic P1' were synthesized by ring-closing metathesis, using both microwave and conventional heating. Besides formation of the expected macrocycles in cis and trans configuration as major products, both ring-contracted and double-bond migrated isomers were obtained, in particular during formation of the smaller rings (14- and 15-membered rings). All inhibitors had K(i)-values in the nanomolar range, but only one inhibitor type was improved by rigidification. The loss in inhibitory effect can be attributed to a disruption of the beneficial π-π interaction between the P2 fragment and H57, which proved to be especially deleterious for the d-phenylglycine epimers. 相似文献
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