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971.
972.
Chekhovskaia GV Shchelkanova EIu Smirnova NI 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2000,(1):34-37
On the basis of V. cholerae strain P16064, serogroup O139, spontaneous and transposon mutants with the stable lose of the capacity for producing the polysaccharide capsule, but retaining antigen O139, have been obtained. As revealed in this study, capsular and noncapsular strains differ in their sensitivity to cholera phages 20 and Inaba, as well as in agglutination with O139-antiserum. These data make it possible to use of bacteriophages for the differentiation of capsular and noncapsular strains. The use of noncapsular mutants ensure obtaining rabbit O139-antisera with higher antibody titer. 相似文献
973.
Smirnova NI Livanova LF Chekhovskaia GV Eroshenko GA Lazovskiĭ IuV Zakharova TL 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2000,(3):47-51
To find out stable and effective producers of major protective antigens intended for use as components of cholera chemical vaccine against V. cholerae strains of serogroups O and O139, the comparative analysis of the production of cholera toxin, toxin-coregulated pili (TCP), antigens O1 and O139, polysaccharide capsule and outer membrane protein OmpU in different V. cholerae strains groups O1 and O139 has been made. V. cholerae strain KM68, serogroup O1, has been found capable of the production of antigen O1, serovar Ogawa, protein OmpU at a sufficiently high level and the hyperproduction of cholera toxin and TCP, and thus suitable for use in the manufacture of cholera bivalent vaccine as the source of these antigens. Specially selected alysogenic noncapsular strain KM137 of serogroup O139, characterized by a high and stable level of the biosynthesis of this somatic antigen when grown in both laboratory and production conditions, may serve as the produces of antigen O139. 相似文献
974.
Mironov AIu Savitskaia KI Vorob'ev AA 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2000,(5):11-15
The microflora of clinical material (blood, wound discharge, abdominal exudate, pleural exudate, milk in mastitis, as well as feces in dysbiosis of the large intestine), obtained from patients with purulent septic diseases in the Moscow region was studied. The study revealed the change of microflora from surface biotopes to closed cavities of the body. Gram-positive cocci, primarily coagulase-negative staphylococci, mainly epidermal, were shown to play the leading etiological role in the development of purulent septic diseases. The development of bacteriemia was found to be caused by the penetration of staphylococci from local pyoinflammatory foci (appearing in purulent mastitis, wounds, peritonitis, pleural empyema and pleuritis), as well as by the translocation of staphylococci and Escherichia coli from the large intestine in dysbiosis. 相似文献
975.
Vakhitov TIa Iashina OIu Petrov LN Koroliuk AM 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2000,(3):20-24
The effect of the preparation of E. coli M-17 low-molecular exometabolites (Actoflor), containing growth autostimulators, on the growth of pure cultures of E. coli M-17 E. coli K-12, Salmonella enteritidis, Serratia marcescens and Bifidobacterium adolescentis MC-42 was studied. This preparation was shown to stimulate the growth of all above-mentioned bacteria. The addition of Actoflor also led to the acceleration of growth in the cultivation of mixed cultures of E. coli M-17 with E. coli K-12 (or S. enteritidis), the producer strain (E. coli M-17) showing the highest degree of acceleration. Moreover, the action of Actoflor led to the elimination of competitor strains and to the increase of the antagonistic activity of E. coli M-17. Actoflor may be supposedly used as a therapeutic or prophylactic remedy. 相似文献
976.
Onishchenko GG Aĭdinov GT Moskvitina EA Lomov IuM Tikhonov NG Prometnoĭ VI Shvager MM Ryzhkov VIu Savchenko PP Dmitrieva TA Batashev VV Pukhov IuM Pichurina NL Ivanova NG Gavrinev SA Kovalev EV Kipaĭkin VA Pauk VL Emel'ianova ZN Orekhov IV Lipkovich AD Stakheev VV Trepel' VG Usatkin AV Markov VI Borisevich IV Merkulov VA Makhlaĭ AA Vasil'ev NT Mishan'kin BN Vodop'ianov SO Mazrukho TV Badunenko VP 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2000,(2):36-42
The results of the epidemiological analysis of the outbreak of hemorrhagic fever which was caused by Crimean-Congo hemorrhagic fever virus and occurred during the period of July 3-19, 1999, in the Oblivskaya district of Rostov Province are presented. The specific epidemiological features of the outbreak have been determined. The possible versions of the appearance of the focus of infection and the role of Ixodes ticks in the circulation of the infective agent are discussed. 相似文献
977.
978.
Khodakevich L Kobyshcha Iu Bezruchenko-Novachuk M 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2000,(4):61-65
Grounds for the necessity of the formation of effective approaches to the prevention of HIV epidemic with a view to counteract the rapid spread of infection in East European countries are presented. Attention is specially drawn to fact that the epidemic will not be limited by drug users, but will quickly cross the boundaries of this group. The development of preventive measures must be based on the use of the world experience. New effective approaches to work with vulnerable groups (the strategy of harm reduction, work in communities, consultations, etc.) must be widely used. The program of actions of the UNAIDS European Section, stipulating the strengthening and extension of the potential of the countries in the region for the effective counteraction to the epidemic, is formulated. As priority areas, activities covering vulnerable groups and with young people have been determined. The strategy of UNAIDS is realized through 20 UN theme groups on HIV/AIDS in the countries of Eastern Europe. Special attention is drawn to the creation of methodological networks. The creation of a unified strategic plan for counteraction the epidemic of HIV/AIDS is regarded by UNAIDS as its perspective for the future. 相似文献
979.
Opportunistic microorganisms in respiratory tract diseases in patients of the Moscow Province region
Mironov AIu Savitskaia KI Vorob'ev AA 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2000,(1):81-84
The respiratory tract microflora in patients with inflammatory processes of the upper and lower respiratory tracts in the Moscow Province region has been studied. Changes in the microflora were found to occur in patients with pyoinflammatory diseases (PID) of the upper and lower respiratory tracts have been found to occur. Gram-positive cocci, mainly staphylococci and streptococci, were shown to play the leading etiological role in the development of PID. As revealed in this study, the microorganisms detected in the inflammations of the upper and lower respiratory tracts are coagulase-negative staphylococci, mainly S. epidermidis, as well as enterococci and streptococci belonging to the group viridans. 相似文献
980.
Rybin VG Shul'gina LV Kuklev DV Byval'tseva TM Blinnov IuG Davletshina TA Akulin VN 《Prikladnaia biokhimiia i mikrobiologiia》2000,36(4):492-496
The induction of antimicrobial activity of a new preparation, an aqueous fraction of water-oil emulsion oxidized by air oxygen, was studied. The effect of various factors (the degree of unsaturation of the initial oil and the content of oil oxidation products in obtained preparation) on the antimicrobial activity was determined. The antimicrobial activity of the preparation was induced by oil oxidation. The preparation produced from sardine Sardinops melanostica oil (33.95% of polyunsaturated fatty acids) displayed the highest antimicrobial activity. The antimicrobial activity was shown in water-soluble oil oxidation products. 相似文献