Dependence of hypoxic changes in macro- and micro-hemodynamics on the activity of adrenoreceptors in the skeletal muscle in hypothermia

Dependence of hypoxic changes of macro- and microcirculation on the activity of adrenergic receptors in the cooled organism was studied on decentralized shank of cat under perfusion with constant blood flow. After cooling of cat (to 30 degrees C) and blockade of alpha-adrenoreceptors hypoxic hypoxia (10% O2 in N2) caused (a) much greater reduction of precapillary resistance of shank, (b) more striking (by 3 times) increase of capillary filtration coefficient and (c) the increase of capillary pressure and postcapillary resistance in contrast to their decrease to hypoxia under hypothermia before alpha-blockade. Beta-adrenoreceptor blockade had no influence on the changes of the resistance and exchange function of skeletal muscle blood vessels evoked by hypoxia under cooling.     

Interaction of T-activin with peritoneal macrophages

The action of T-activin on peritoneal macrophages of CBA mice after its introduction into the animals has been studied. In intact mice the phagocytic activity of macrophages and their resistance to the cytopathogenic action of Salmonella typhimurium live cells remains unchanged. The injection of corpuscular pertussis vaccine into mice leads to a decrease in the resistance of macrophages to the action of salmonellae. The simultaneous injection of T-activin into mice in doses of 0.1 and 1.0 microgram per animal abolishes the damaging action of the vaccine. The analysis of the in vitro action of T-activin on macrophages of intact mice revealed that the preliminary incubation of cells with the preparation sharply increases their resistance to the action of salmonellae, while its introduction simultaneously with bacteria or after them rapidly leads to the death of macrophages. The action of T-activin is supposed to be linked with triggering the biosynthetic processes mediating the resistance of macrophages to the cytopathogenic action of salmonellae.     

Effect of monochromatic red helium-neon laser on the morphology of zymosan arthritis in rats

Histological and electron microscopy findings suggesting the effect of monochromatic coherent polarized red light on zymosan arthritis in rats are presented. The data obtained have confirmed that treatment of inflamed joints with laser rays exerts an evident therapeutic effect which is determined by activated function of macrophages and fibroblasts of the synovial membrane, resulting in a more rapid change of inflammation phases. Analysis of the cases where the rats were treated with ordinary red light has revealed no essential differences in the joints of the control and irradiated groups.     

Enzymes of cyclic nucleotide degradation in eye tissues during experimental ocular herpes

Activity of phosphodiesterases disintegrating cAMP and cGMP in the cornea, sclera and ciliary body was investigated in health and in different stages of experimental herpetic keratitis. The problems concerning the role of the cyclase system in the pathogenesis of herpetic keratitis and the possibility of applying some of the drugs to the disease treatment are discussed.     

Comparative analysis of the HBsAg level in human blood preparations

A total of 218 batches of blood preparations produced from different raw materials have been studied by means of enzyme immunoassay kits (Abbott Laboratories, USA). The assays have revealed that the preparations under study are nonstandard with respect to the content and isolation rate of HBsAg, the marker of hepatitis B virus. These data necessitate search for the ways of improving the quality of blood preparations.     

Evaluation of cell proliferation kinetics by the differential staining of the sister chromatids at 2 points of cell fixation

Lymphocyte proliferation in blood cultures from 23 normal individuals was studied. A simple method of evaluation of cell proliferative kinetics with sister chromatid differential staining using two different points of cell harvesting was suggested. It was shown that different composition of tissue culture medium plays a significant role during the cell exit from G0 stage, the mean doubling generation time of cellular population remaining constant.