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961.
Takamiya R Takahashi M Park YS Tawara Y Fujiwara N Miyamoto Y Gu J Suzuki K Taniguchi N 《American journal of physiology. Cell physiology》2005,288(2):C253-C259
Amyotrophic lateral sclerosis (ALS) involves the progressive degeneration of motor neurons in the spinal cord and the motor cortex. It has been shown that 1520% of patients with familial ALS (FALS) have defects in the Sod1 gene, which encodes Cu,Zn-superoxide dismutase (SOD). To elucidate the pathological role of mutated Cu,Zn-SOD, we examined the issue of whether mutated Cu,Zn-SOD affects the cell cycle. Mouse neuroblastoma Neuro-2a cells were transfected with human wild-type or mutated (G37R, G93A) Cu,Zn-SOD. Mutated, Cu,Zn-SOD-transfected cells exhibited marked retardation in cell growth and G2/M arrest. They also displayed lower reactivity to phalloidin, indicating that the cytoskeleton was disrupted. Immunoprecipitation, two-dimensional gel electrophoresis, and Western blot analysis indicated that mutated Cu,Zn-SOD associates with actin. Similar results were obtained by in vitro incubation experiments with purified actin and mutated Cu,Zn-SOD (G93A). These results suggest that mutated Cu,Zn-SOD in FALS causes cytoskeletal changes by associating with actin, which subsequently causes G2/M arrest and growth retardation. amyotrophic lateral sclerosis; copper; zinc superoxide dismutase; G2/M arrest; neurodegenerative disease 相似文献
962.
963.
Yuji Taniguchi Cornelia Appiah-Kwarteng Mami Murakami Junpei Fukumoto Kisaburo Nagamune Tomohide Matsuo Tatsunori Masatani Hirotaka Kanuka Tokio Hoshina Katsuya Kitoh Yasuhiro Takashima 《Parasitology international》2018,67(5):587-592
The virulence of a type III Toxoplasma gondii strain isolated in Japan and designated here as TgCatJpGi1/TaJ was examined in mice and micro minipigs in this study. Despite its type III genotype, oral or intraperitoneal inoculation of cysts from it resulted in severe virulence in C57BL/6J and BALB/c mice. In contrast, mice inoculated with a high dose of TgCatJpGi1/TaJ tachyzoites showed no obvious clinical signs of infection, and all of them survived for >21?days post-inoculation. Furthermore, no clinical signs of infection were seen when micro minipigs were inoculated with 900 cysts. Interestingly, our allelic type screening of the virulence-related rop5, rop16, rop17, and rop18 genes, as based on restriction fragment length polymorphism analysis (RFLP), revealed that the RFLP patterns for TgCatJpGi1/TaJ were identical to those from nonvirulent type III parasites. These results suggest that TgCatJpGi1/TaJ possesses an unknown virulence factor or factors. 相似文献
964.
965.
Saya Ito Naruhiro Kayukawa Takashi Ueda Hidefumi Taniguchi Yukako Morioka Fumiya Hongo Osamu Ukimura 《Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms》2018,1861(9):794-802
The androgen receptor (AR) promotes growth of prostate cancer cells by controlling the expression of target genes. This study showed that MRG domain binding protein (MRGBP) accelerated AR-mediated transactivation. We first showed that MRGBP promoted growth of AR-positive prostate cancer cells. MRGBP increased the expression of certain AR target genes, including KLK3 and TMPRSS2, and it associated with AR binding regions of these genes during androgen treatment. Furthermore, MRGBP interacted with MRG15 and TIP60 in prostate cancer cells. Androgen-stimulated AR enhanced histone H3K4me1 or H3K4me3 levels at AR binding regions. MRGBP was recruited to active gene regions through its binding with H3K4me1/3 by MRG15. Then, MRGBP promoted recruitment of TIP60 and acetylation of histone variant H2A.Z at the location of AR binding. Accordingly, AR occupancy of the AR binding regions was increased by MRGBP. Together, these results suggest that MRGBP promotes activation of AR-associated enhancer and promoter regions through an epigenetic mechanism. 相似文献
966.
Optical study of spatiotemporal inhibition evoked by two-tone sequences in the guinea pig auditory cortex 总被引:1,自引:0,他引:1
J. Horikawa Y. Hosokawa M. Nasu I. Taniguchi 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1997,181(6):677-684
Spatiotemporal response patterns in the anterior and dorsocaudal fields of the guinea pig auditory cortex after two-tone
sequences were studied in anesthetized animals (Nembutal 30 mg kg−1) using an optical recording method (voltage-sensitive dye RH795, 12 × 12 photodiode array). Each first (masker) and second
(probe) tone was 30 ms long with a 10-ms rise-fall time. Masker-probe pair combinations of the same or different frequencies
with probe delays of 30–150 ms were presented to the ear contralateral to the recording side. With same-frequency pairs, responses
to the probe were inhibited completely after probe delays of less than 50 ms and the inhibition lasted for more than 150 ms,
and the inhibition magnitudes in different isofrequency bands of the anterior field were essentially the same. With different-frequency
(octave-separated) pairs, responses to the probe were not inhibited completely even after probe delays as short as 30 ms,
and the inhibition lasted only for 110–130 ms. Inhibition magnitudes were different from location to location.
Accepted: 4 August 1997 相似文献
967.
Establishment of internal-image anti-idiotype monoclonal antibodies to a human antibody to lung cancer 总被引:4,自引:0,他引:4
Hiroaki Saito Masaru Taniguchi Toshio Fukasawa Yutaka Yamaguchi T. Fujisawa 《Cancer immunology, immunotherapy : CII》1997,44(2):83-87
Internal-image anti-idiotype antibodies are expected to enhance anticancer effector mechanisms in vivo. The objective of
this study was to establish hybridomas producing anti-idiotype monoclonal antibodies against a human monoclonal antibody (hmAb)
4G12 that reacts strongly with lung squamous cell carcinomas. BALB/c female mice 6 weeks old were immunized with 4G12. Splenocytes
were hybridized with P3U1 cells and hybrid cells secreting anti-4G12 hmAb were cloned. Two clones reacted with 4G12 hmAb but
not with 3H12 IgM hmAb, human IgM, human serum or fetal calf serum. These two Ab2 antibodies (IgG1κ) 2B12 and 2H1 demonstrated
91.5% and 90.3% inhibition in their reactivity with radiolabelled 4G12 on PC10 cells, indicating that 2B12 and 2H1 antibodies
were of the Ab2β type. In criss-cross inhibition assays, the binding of 2B12 or 2H1 to 4G12 was not inhibited by 2H1 or 2B12.
Thus 2B12 and 2H1 were thought to recognize the different epitopes on the antigen-binding sites. Antisera against 2B12 and
2H1 demonstrated specific reactivity to PC10 cells. The two Ab2β antibodies, 2B12 and 2H1, express internal images of lung
squamous cell carcinoma recognized by the 4G12 antibody and may be useful for cancer immunotherapy.
Received: 20 September 1996 / Accepted: 2 January 1997 相似文献
968.
Akinori Hirashima Jun Tomita Canping Pan Eiji Taniguchi Morifusa Eto 《Bioorganic & medicinal chemistry》1997,5(12):2121-2128
The quantitative structure-activity relationship (QSAR) of octopaminergic 2-(arylimino)thiazolidines (AITs) and 2-(arylimino)oxazolidines (AIOs) against the thoracic nerve cord of the American cockroach, Periplaneta americana L., was analysed using reported physicochemical parameters and regression analysis. The more electron-donating, the less bulky at m-position, and the more hydrophobic the substituent, the greater the activity. The plots of observed log Vmax values against calculated log Vmax values having substituents on the m-position deviated downwards from those of compounds having substituents at the o- and/or p-positions. The more hydrophobic and the more electron-withdrawing the substituent, the greater the activity. AIO with a 2,3,4-trichlorophenyl group (58) was more active than its thiazolidine derivative, 2-(2,3,4-trichlorophenylimino)thiazolidine (38) in terms of Vmax:Vmax of 58 was 30% relative to octopamine (OA), whereas that of 38 has been 9% relative to OA, respectively. Superimposition of energy-minimized OA and 58 revealed structural and conformational similarities that might account for the high activity of 58. 相似文献
969.
Morphological and genetic characteristics of sea bass,Lateolabrax japonicus, from the Ariake Sea, Japan 总被引:1,自引:1,他引:0
Sea bass,Lateolabrax japonicus, from the Ariake Sea, characterized by black dots on the lateral body region as in the Chinese sea bass,L. sp., were examined and compared morphologically and genetically withL. japonicus andL. sp. Some meristic characters of the Ariake form tended to fall midway between values for the two former species. Genetic
features, evaluated by isozyme analyses, indicated that the Ariake form as represented a simple Mendelian population, there
being no significant differences from a Hardy-Weinberg equilibrium according to chi-square tests. Although some extreme differences
in allelic frequencies were found at some loci betweenL. japonicus andL. sp., the Ariake form possessed many heterozygotes at thePROT-1
* locus, in addition to allelic frequencies at some loci conforming to those ofL. sp. Average allele numbers per locus, rate of polymorphic loci and average heterozygosity of the Ariake form were higher
than for eitherL. japonicus orL. sp., indicating high genetic variation in the former. The results suggested that the Ariake population is genetically independent
of other populations ofL. japonicus, but might be genetically influenced byL. sp. 相似文献