全文获取类型
收费全文 | 3927篇 |
免费 | 262篇 |
国内免费 | 1篇 |
出版年
2022年 | 19篇 |
2021年 | 31篇 |
2020年 | 15篇 |
2019年 | 22篇 |
2018年 | 42篇 |
2017年 | 29篇 |
2016年 | 55篇 |
2015年 | 87篇 |
2014年 | 92篇 |
2013年 | 197篇 |
2012年 | 173篇 |
2011年 | 210篇 |
2010年 | 121篇 |
2009年 | 135篇 |
2008年 | 205篇 |
2007年 | 177篇 |
2006年 | 199篇 |
2005年 | 208篇 |
2004年 | 181篇 |
2003年 | 176篇 |
2002年 | 199篇 |
2001年 | 157篇 |
2000年 | 156篇 |
1999年 | 124篇 |
1998年 | 58篇 |
1997年 | 57篇 |
1996年 | 39篇 |
1995年 | 33篇 |
1994年 | 40篇 |
1993年 | 31篇 |
1992年 | 86篇 |
1991年 | 84篇 |
1990年 | 76篇 |
1989年 | 85篇 |
1988年 | 73篇 |
1987年 | 66篇 |
1986年 | 70篇 |
1985年 | 48篇 |
1984年 | 46篇 |
1983年 | 45篇 |
1982年 | 34篇 |
1981年 | 26篇 |
1980年 | 27篇 |
1979年 | 28篇 |
1978年 | 14篇 |
1977年 | 23篇 |
1976年 | 19篇 |
1975年 | 14篇 |
1972年 | 8篇 |
1968年 | 10篇 |
排序方式: 共有4190条查询结果,搜索用时 15 毫秒
991.
The configuration of the Δ23-bond of cyclosadol (24-methyl-23-dehydrocycloartanol) was determined as E based on the 1H and 13C NMR co 相似文献
992.
993.
We previously isolated a nuclear 5.7 kb genomic fragment carrying the NAM7/UPF1 gene, which is able to suppress mitochondrial splicing deficiency when present in multiple copies. We show here that an immediately adjacent gene ISF1 (Increasing Suppression Factor) increases the efficiency of the NAM7/UPF1 suppressor activity. The ISF1 gene has been independently isolated as the MBR3 gene and comparison of the ISF1 predicted protein sequence with data libraries revealed a significant similarity with the MBRI yeast protein. The ISF1 and NAM7 genes are transcribed in the same direction, and RNase mapping allowed the precise location of their termini within the intergenic region to be determined. The ISF1 gene is not essential for cell viability or respiratory growth. However as for many mitochondrial genes, ISF1 expression is sensitive to fermentative repression; in contrast expression of the NAM7 gene is unaffected by glucose. We propose that ISF1 could influence the NAM7/UPF1 function, possibly at the level of mRNA turnover, thus modulating the expression of nuclear genes involved in mitochondrial biogenesis. 相似文献
994.
Photosystem I core complexes were isolated from spinach photosystem I particles after heat treatment in the presence of 50% (v/v) ethylene glycol (heat/EG treatment). The core complex from 58 degrees C/EG-treated particles was composed of polypeptides with apparent molecular masses of 63, 60 and 5 kDA; this complex contained the iron sulfur center Fx but lacked center FA and FB. The core complex obtained from the 70 degrees C/EG-treated preparation lacked FX and contained a lesser amount of the 5 kDa polypeptide. The N-terminal amino acid sequence of the 5 kDa polypeptide did not correspond to the sequence derived from any possible reading frame in the chloroplast DNA of liverwort or tobacco. Twelve of the first 29 N-terminal amino acids were hydrophobic, suggesting that this protein is intrinsic to the photosystem I reaction center. 相似文献
995.
996.
S Shuto S Ueda H Itoh E Endo K Fukukawa S Imamura M Tsujino A Matsuda T Ueda 《Nucleic acids symposium series》1986,(17):73-76
Phospholipase D from Streptomyces effectively catalyzed the transfer reaction of the phosphatidyl residue from phosphatidylcholine to the 5'-hydroxyl group of nucleosides. A variety of 5'-phosphatidylnucleosides were prepared by this reaction in high yields. Some of them showed marked antitumor activities in mice. 相似文献
997.
998.
999.
Selective cytochrome P450 (CYP) 1B1 inhibition has potential as an anticancer strategy that is unrepresented in the current clinical arena. For development of a selective inhibitor, we focused on the complexity caused by sp3-hybridized carbons and synthesized a series of benzo[h]chromone derivatives linked to a non-aromatic B-ring using α-naphthoflavone (ANF) as the lead compound. Ring structure comparison suggested compound 37 as a suitable cyclohexyl-core with improved solubility. Structural evolution of 37 produced the azide-containing cis-49a, which had good properties in three important respects: (1) selectivity for CYP1B1 over CYP1A1 and CYP1A2 (120-times and 150-times, respectively), (2) greater inhibitory potency of >2 times that of ANF, and (3) improved solubility. The corresponding aromatic B-ring compound 59a showed low selectivity and poor solubility. To elucidate the binding mode, we performed X-ray crystal structure analysis, which revealed the interaction mode and explained the subtype selectivity of cis-49a. 相似文献
1000.
An irreversible inhibitor of peptidyl-prolyl cis/trans isomerase Pin1 and evaluation of cytotoxicity
Naoya Ieda Kaoru Itoh Yasumichi Inoue Yusuke Izumiya Mitusyasu Kawaguchi Naoki Miyata Hidehiko Nakagawa 《Bioorganic & medicinal chemistry letters》2019,29(3):353-356
Pin1 (protein interacting with never in mitosis A-1) is a member of the peptidyl prolyl isomerase (PPIase) family, and catalyzes cis-trans isomerization of pThr/Ser-Pro amide bonds. Because Pin1 is overexpressed in various cancer cell lines and promotes cell growth, it is considered a target for anticancer agents. Here, we designed and synthesized a covalently binding Pin1 inhibitor (S)-2 to target Pin1’s active site. This compound inhibited Pin1 in protease-coupled assay, and formed a covalent bond with Cys113 of Pin1, as determined by ESI-MS. The acetoxymethyl ester of (S)-2, i.e., 6, suppressed cyclin D1 expression in human prostate cancer PC-3 cells, and exhibited cytotoxicity. Pin1-knockdown experiments indicated that a target for the cytotoxicity of 6 is Pin1. 相似文献